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AB175750

Donkey Anti-Rat IgG H&L (Alexa Fluor® 750) preadsorbed

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(2 Publications)

Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

View Alternative Names

Ig gamma-1 chain C region

1 Images
Western blot - Donkey Anti-Rat IgG H&L (Alexa Fluor® 750) preadsorbed (AB175750)
  • WB

Lab

Western blot - Donkey Anti-Rat IgG H&L (Alexa Fluor® 750) preadsorbed (AB175750)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with ab6160 overnight at 4°C. Antibody binding was detected using ab175750 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (<a href='/en-us/products/primary-antibodies/tubulin-antibody-yl1-2-loading-control-ab6160'>ab6160</a>) at 1 µg/mL

All lanes:

HeLa Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Donkey Anti-Rat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175750) at 1/10000 dilution

Predicted band size: 36 kDa

false

Key facts

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Human

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Applications

WB

applications

Clonality

Polyclonal

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }

Product details

We batch test Donkey Anti-Rat IgG H&L (Alexa Fluor® 750) preadsorbed, ab175750 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Rat IgG H&L (Alexa Fluor® 750) preadsorbed, ab175750 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

iScience 27:111239 PubMed39569362

2024

Molecular diversity and migration of GABAergic neurons in the developing ventral midbrain.

Applications

Unspecified application

Species

Unspecified reactive species

Özge Düdükcü,Divya D A Raj,Lieke L van de Haar,Laurens M Grossouw,Louisa E Linders,Oxana Garritsen,Youri Adolfs,Nicky C H van Kronenburg,Mark H Broekhoven,Troy H W Kapteijns,Frank J Meye,R Jeroen Pasterkamp

Stem cells international 2023:7397819 PubMed37705699

2023

Intravenous Administration of Human Umbilical Cord Mesenchymal Stromal Cells Leads to an Inflammatory Response in the Lung.

Applications

Unspecified application

Species

Unspecified reactive species

Alejandra Hernandez Pichardo,Bettina Wilm,Neill J Liptrott,Patricia Murray
View all publications

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