Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.
Donkey
Rat
IgG
Heavy & Light chains
Mouse, Human
Alexa Fluor® 750
Ex: 749nm, Em: 775nm
WB
Polyclonal
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/10000 | Notes - |
Ig gamma-1 chain C region
Suitable for WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.
Ig gamma-1 chain C region
Donkey Anti-Rat IgG H&L (Alexa Fluor® 750) preadsorbed
Donkey
Rat
IgG
Heavy & Light chains
Mouse, Human
Alexa Fluor® 750
Ex: 749nm, Em: 775nm
WB
Polyclonal
Yes
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.
Liquid
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Affinity purification Immunogen
Antiserum was cross adsorbed using human and mouse immunosorbents to remove cross reactive antibodies. The antibody to rat IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
We batch test Donkey Anti-Rat IgG H&L (Alexa Fluor® 750) preadsorbed, ab175750 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success using Donkey Anti-Rat IgG H&L (Alexa Fluor® 750) preadsorbed, ab175750 in these applications.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Terms & Conditions.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk blocking buffer before being incubated with Anti-Tubulin antibody [YL1/2] - Loading Control ab6160 overnight at 4°C. Antibody binding was detected using ab175750 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-Tubulin antibody [YL1/2] - Loading Control (Anti-Tubulin antibody [YL1/2] - Loading Control ab6160) at 1 µg/mL
All lanes: HeLa Whole Cell Lysate at 10 µg
All lanes: Western blot - Donkey Anti-Rat IgG H&L (Alexa Fluor® 750) preadsorbed (ab175750) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 36 kDa
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