AB102259

Donkey Anti-Rat IgG H&L (Biotin) preadsorbed

Name: Donkey Anti Rat (IgG) secondary antibody preadsorbed Biotin (ab102259) | Abcam
Brand: Abcam Limited
SKU: AB102259

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(3 Publications)

Suitable for ICC, IHC-P, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.

View Alternative Names

Ig gamma-1 chain C region

Key facts

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human

Pre-adsorbed

Yes

Conjugation

Biotin

Excitation/Emission

Applications

ICC, IHC-P, WB, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"(Primary ELISA)." }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"(Chemiluminescent)." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ig gamma-1 chain C region

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

eNeuro 11: PubMed39349059

2024

BMP Antagonist Gremlin 2 Regulates Hippocampal Neurogenesis and Is Associated with Seizure Susceptibility and Anxiety.

Applications

Unspecified application

Species

Unspecified reactive species

Nicolette B Frazer,Garrett A Kaas,Caroline G Firmin,Eric R Gamazon,Antonis K Hatzopoulos

The Journal of neuroscience : the official journal of the Society for Neuroscience 41:4795-4808 PubMed33906900

2021

Mutually Repulsive EphA7-EfnA5 Organize Region-to-Region Corticopontine Projection by Inhibiting Collateral Extension.

Applications

Unspecified application

Species

Unspecified reactive species

Tokuichi Iguchi,Yuichiro Oka,Misato Yasumura,Minoru Omi,Kazuki Kuroda,Hideshi Yagi,Min-Jue Xie,Manabu Taniguchi,Martin Bastmeyer,Makoto Sato

PloS one 11:e0162144 PubMed27589391

2016

Novel Rat Model of Repetitive Portal Venous Embolization Mimicking Human Non-Cirrhotic Idiopathic Portal Hypertension.

Applications

Unspecified application

Species

Unspecified reactive species

Sabine Klein,Christian Hinüber,Kanishka Hittatiya,Robert Schierwagen,Frank Erhard Uschner,Christian P Strassburg,Hans-Peter Fischer,Ulrich Spengler,Jonel Trebicka

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Donkey Anti-Rat IgG H&L (Biotin) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (3)

Product promise