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AB102260

Donkey Anti-Rat IgG H&L (DyLight® 488) preadsorbed

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(4 Publications)

Suitable for IHC-P, ICC, Flow Cyt, IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.

View Alternative Names

Ig gamma-1 chain C region

Key facts

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human

Pre-adsorbed

Yes

Conjugation

DyLight® 488

Excitation/Emission

Ex: 493nm, Em: 518nm

Applications

Flow Cyt, IF, IHC-P, ICC

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"<p></p>" }, "IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 488.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

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Target data

See full target information Ig gamma-1 chain C region
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Human molecular genetics 31:189-206 PubMed34392367

2021

Myeloid cell-mediated targeting of LIF to dystrophic muscle causes transient increases in muscle fiber lesions by disrupting the recruitment and dispersion of macrophages in muscle.

Applications

Unspecified application

Species

Unspecified reactive species

Ivan Flores,Steven S Welc,Michelle Wehling-Henricks,James G Tidball

Journal of immunology (Baltimore, Md. : 1950) 205:1664-1677 PubMed32817369

2020

Differential Effects of Myeloid Cell PPARδ and IL-10 in Regulating Macrophage Recruitment, Phenotype, and Regeneration following Acute Muscle Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Steven S Welc,Michelle Wehling-Henricks,Jacqueline Antoun,Tracey T Ha,Isabella Tous,James G Tidball

Nature communications 10:2788 PubMed31243277

2019

Targeting a therapeutic LIF transgene to muscle via the immune system ameliorates muscular dystrophy.

Applications

Unspecified application

Species

Unspecified reactive species

Steven S Welc,Ivan Flores,Michelle Wehling-Henricks,Julian Ramos,Ying Wang,Carmen Bertoni,James G Tidball

Cell reports 3:427-41 PubMed23403291

2013

c-MYC-induced sebaceous gland differentiation is controlled by an androgen receptor/p53 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Denny L Cottle,Kai Kretzschmar,Pawel J Schweiger,Sven R Quist,Harald P Gollnick,Ken Natsuga,Satoru Aoyagi,Fiona M Watt
View all publications
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Product promise

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