Suitable for Flow Cyt, ICC, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.
Donkey
Rat
IgG
Heavy & Light chains
Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human
DyLight® 550
Flow Cyt, ICC, IHC-P
Polyclonal
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Application ICC | Reactivity Reacts | Dilution info 1/50.00000 - 1/500.00000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/50.00000 - 1/500.00000 | Notes DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments. |
Ig gamma-1 chain C region
Suitable for Flow Cyt, ICC, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.
Ig gamma-1 chain C region
Donkey Anti-Rat IgG H&L (DyLight® 550) preadsorbed
Donkey
Rat
IgG
Heavy & Light chains
Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human
DyLight® 550
Flow Cyt, ICC, IHC-P
Polyclonal
Yes
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.
IgG
Liquid
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA
Affinity purification Immunogen
Antiserum was cross adsorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
Blue Ice
+4°C
DyLight® 550 replaces DyLight® 549.
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