AB102261

Donkey Anti-Rat IgG H&L (DyLight® 550) preadsorbed

Name: Donkey Anti Rat (IgG) secondary antibody preadsorbed DyLight® 550 (ab102261)
Brand: Abcam Limited
SKU: AB102261

Be the first to review this product! Submit a review

(6 Publications)

Suitable for Flow Cyt, ICC, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 6 publications.

View Alternative Names

Ig gamma-1 chain C region

Key facts

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human

Pre-adsorbed

Yes

Conjugation

DyLight® 550

Excitation/Emission

Ex: 562nm, Em: 576nm

Applications

IHC-P, ICC, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to bovine, chicken, goat, horse, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"DyLight® 550 replaces DyLight® 549. The DyLight® 550 excitation is 562nm and the emission is 576nm. DyLight® 550 provides the same orange-to-red fluorescence and photostability as the DyLight® 549 dye. Please refer to the vial in order to check whether the product you received is DyLight® 549 or DyLight® 550. This information however will have no impact on your experiments." } } }

style

left-column

Product details

DyLight® 550 replaces DyLight® 549.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

style

left-column

style

left-column

Product protocols

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

See full target information Ig gamma-1 chain C region

style

left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Genes to cells : devoted to molecular & cellular mechanisms 29:854-875 PubMed39109760

2024

Lineage tracing using Wnt2b-2A-CreERT2 knock-in mice reveals the contributions of Wnt2b-expressing cells to novel subpopulations of mesothelial/epicardial cell lineages during mouse development.

Applications

Unspecified application

Species

Unspecified reactive species

Masanori Takahashi,Takayuki Isagawa,Tatsuyuki Sato,Norihiko Takeda,Kiyoshi Kawakami

American journal of translational research 14:3879-3892 PubMed35836896

2022

Cryopreserved allogenic fibroblast sheets: development of a promising treatment for refractory skin ulcers.

Applications

Unspecified application

Species

Unspecified reactive species

Soichi Ike,Koji Ueno,Masashi Yanagihara,Takahiro Mizoguchi,Takasuke Harada,Kotaro Suehiro,Hiroshi Kurazumi,Ryo Suzuki,Tomoko Kondo,Tomoaki Murata,Bungo Shirasawa,Noriyasu Morikage,Kimikazu Hamano

Circulation research 129:992-1005 PubMed34615369

2021

SMC-Derived Hyaluronan Modulates Vascular SMC Phenotype in Murine Atherosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Felicia Hartmann,Daniel J Gorski,Alexandra A C Newman,Susanne Homann,Anne Petz,Katherine M Owsiany,Vlad Serbulea,Yu-Qing Zhou,Rebecca A Deaton,Michelle Bendeck,Gary K Owens,Jens W Fischer

Nature metabolism 3:166-181 PubMed33619382

2021

Multiple cell types contribute to the atherosclerotic lesion fibrous cap by PDGFRβ and bioenergetic mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra A C Newman,Vlad Serbulea,Richard A Baylis,Laura S Shankman,Xenia Bradley,Gabriel F Alencar,Katherine Owsiany,Rebecca A Deaton,Santosh Karnewar,Sohel Shamsuzzaman,Anita Salamon,Mahima S Reddy,Liang Guo,Aloke Finn,Renu Virmani,Olga A Cherepanova,Gary K Owens

Nature communications 10:967 PubMed30814500

2019

Perivascular cell-specific knockout of the stem cell pluripotency gene Oct4 inhibits angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel L Hess,Molly R Kelly-Goss,Olga A Cherepanova,Anh T Nguyen,Richard A Baylis,Svyatoslav Tkachenko,Brian H Annex,Shayn M Peirce,Gary K Owens

American journal of physiology. Heart and circulat 312:H943-H958 PubMed28283548

2017

Smooth muscle cell-specific deletion of unexpectedly leads to impaired development of advanced atherosclerotic lesions.

Applications

Unspecified application

Species

Unspecified reactive species

Brittany G Durgin,Olga A Cherepanova,Delphine Gomez,Themistoclis Karaoli,Gabriel F Alencar,Joshua T Butcher,Yu-Qing Zhou,Michelle P Bendeck,Brant E Isakson,Gary K Owens,Jessica J Connelly

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Rat IgG H&L (DyLight® 550) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (6)

Product promise