AB102182

Donkey Anti-Rat IgG H&L (HRP)

Name: Donkey Anti Rat (IgG) secondary antibody HRP (ab102182) | Abcam
Brand: Abcam Limited
SKU: AB102182
Price: 265 USD
Availability: InStock

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(7 Publications)

Suitable for IHC-P, ICC, WB, ELISA. Ideal for western blot. Cited in 7 publications.

View Alternative Names

Ig gamma-1 chain C region

Key facts

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

HRP

Excitation/Emission

Applications

ELISA, ICC, WB, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/5000", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/5000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"(Colorimetric: 1/5000 - 1/30000; Chemiluminescence: 1/10000 - 1/50000)." }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"(Primary)." } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:106970 PubMed37324527

2023

Restored autophagy is protective against PAK3-induced cardiac dysfunction.

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Ruiz-Velasco,Rida Raja,Xinyi Chen,Haresh Ganenthiran,Namrita Kaur,Nasser Hawimel O Alatawi,Jessica M Miller,Riham R E Abouleisa,Qinghui Ou,Xiangjun Zhao,Oveena Fonseka,Xin Wang,Susanne S Hille,Norbert Frey,Tao Wang,Tamer M A Mohamed,Oliver J Müller,Elizabeth J Cartwright,Wei Liu

iScience 25:103973 PubMed35281739

2022

Paracrine signal emanating from stressed cardiomyocytes aggravates inflammatory microenvironment in diabetic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Namrita Kaur,Andrea Ruiz-Velasco,Rida Raja,Gareth Howell,Jessica M Miller,Riham R E Abouleisa,Qinghui Ou,Kimberly Mace,Susanne S Hille,Norbert Frey,Pablo Binder,Craig P Smith,Helene Fachim,Handrean Soran,Eileithyia Swanton,Tamer M A Mohamed,Oliver J Müller,Xin Wang,Jonathan Chernoff,Elizabeth J Cartwright,Wei Liu

Biochemical pharmacology 197:114899 PubMed34968496

2021

Prolyl oligopeptidase acts as a link between chaperone-mediated autophagy and macroautophagy.

Applications

Unspecified application

Species

Unspecified reactive species

H Cui,S Norrbacka,T T Myöhänen

Biomolecules 11: PubMed34207497

2021

An Antagonistic Peptide of Gpr1 Ameliorates LPS-Induced Depression through the Hypothalamic-Pituitary-Ovarian Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Rongrong Li,Chiyuan Ma,Yue Xiong,Huashan Zhao,Yali Yang,Li Xue,Baobei Wang,Tianxia Xiao,Jie Chen,Xiaohua Lei,Baohua Ma,Jian Zhang

Current biology : CB 30:1002-1010.e4 PubMed32032507

2020

Widely Distributed Neurotensinergic Neurons in the Brainstem Regulate NREM Sleep in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Mitsuaki Kashiwagi,Mika Kanuka,Chika Tatsuzawa,Hitomi Suzuki,Miho Morita,Kaeko Tanaka,Taizo Kawano,Jay W Shin,Harukazu Suzuki,Shigeyoshi Itohara,Masashi Yanagisawa,Yu Hayashi

American journal of physiology. Cell physiology 318:C664-C674 PubMed31851527

2019

R-spondin3 promotes the tumor growth of choriocarcinoma JEG-3 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Zhilong Chen,Juzuo Zhang,Anwen Yuan,Jinyu Han,Lunbo Tan,Zhuoqun Zhou,Huashan Zhao,Rui Su,Binbin Huang,Baobei Wang,Beini Sun,Xiujun Fan,Qing Yang

Nature communications 9:3288 PubMed30120235

2018

Galectin-9 binds IgM-BCR to regulate B cell signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Anh Cao,Nouf Alluqmani,Fatima Hifza Mohammed Buhari,Laabiah Wasim,Logan K Smith,Andrew T Quaile,Michael Shannon,Zaki Hakim,Hossai Furmli,Dylan M Owen,Alexei Savchenko,Bebhinn Treanor

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Donkey Anti-Rat IgG H&L (HRP)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (7)

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Primary Antibodies

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