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Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.
Alternative names=Ig gamma-1 chain C region
- Host species
Donkey
- Target species
Rat
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Minimal cross-reactivity
Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human
- Conjugation
HRP
- Applications
ICC, IHC-P, ELISA, WB
- Clonality
Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info 1/200.00000 - 1/5000.00000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/5000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/100000.00000 | Notes (Primary). |
Application WB | Reactivity Reacts | Dilution info 1/5000.00000 - 1/50000.00000 | Notes Colorimetric: 1/5000 - 1/30000. Chemiluminescent: 1/10000 - 1/50000. |
Recommended products
Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.
Alternative names=Ig gamma-1 chain C region
Key facts
- Description
Donkey Anti-Rat IgG H&L (HRP) preadsorbed
- Host species
Donkey
- Target species
Rat
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Minimal cross-reactivity
Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human
- Conjugation
HRP
- Applications
ICC, IHC-P, ELISA, WB
- Clonality
Polyclonal
- Pre-adsorbed
Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins. Less than 1% cross reactivity to bovine, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.
- Isotype
IgG
- Concentration
Properties
- Form
Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA- Purification technique
Affinity purification Immunogen
- Purification notes
Antiserum was cross absorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactivity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Fragments were conjugated to HRP.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
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