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AB102265

Donkey Anti-Rat IgG H&L (HRP) preadsorbed

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(5 Publications)

Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 5 publications.

View Alternative Names

Ig gamma-1 chain C region

Key facts

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Cow, Human

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission
Applications

WB, ICC, ELISA, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against nonimmunoglobulin serum proteins. Less than 1% cross reactivity to bovine, human, mouse, rabbit and sheep IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/5000", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/5000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"<p>(Primary).</p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"<p>Colorimetric: 1/5000 - 1/30000. Chemiluminescent: 1/10000 - 1/50000.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross absorbed using bovine, chicken, goat, horse, human, mouse, rabbit and sheep immunosorbents to remove cross reactivity. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. Fragments were conjugated to HRP.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

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Target data

See full target information Ig gamma-1 chain C region
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Nature 617:147-153 PubMed36949200

2023

RBFOX2 modulates a metastatic signature of alternative splicing in pancreatic cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Amina Jbara,Kuan-Ting Lin,Chani Stossel,Zahava Siegfried,Haya Shqerat,Adi Amar-Schwartz,Ela Elyada,Maxim Mogilevsky,Maria Raitses-Gurevich,Jared L Johnson,Tomer M Yaron,Ofek Ovadia,Gun Ho Jang,Miri Danan-Gotthold,Lewis C Cantley,Erez Y Levanon,Steven Gallinger,Adrian R Krainer,Talia Golan,Rotem Karni

American journal of hematology 97:1348-1358 PubMed36071577

2022

Erythroferrone contributes to iron mobilization for embryo erythropoiesis in iron-deficient mouse pregnancies.

Applications

Unspecified application

Species

Unspecified reactive species

Veena Sangkhae,Vivian Yu,Richard Coffey,Kimberly O O'Brien,Tomas Ganz,Elizabeta Nemeth

Journal of cellular physiology 235:7094-7106 PubMed32020589

2020

Omega-3 and Omega-6 polyunsaturated fatty acids stimulate vascular differentiation of mouse embryonic stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Amer Taha,Fatemeh Sharifpanah,Maria Wartenberg,Heinrich Sauer

Circulation research 121:368-375 PubMed28611075

2017

Identification and Functional Characterization of Hypoxia-Induced Endoplasmic Reticulum Stress Regulating lncRNA (HypERlnc) in Pericytes.

Applications

Unspecified application

Species

Unspecified reactive species

Florian C Bischoff,Astrid Werner,David John,Jes-Niels Boeckel,Maria-Theodora Melissari,Phillip Grote,Simone F Glaser,Shemsi Demolli,Shizuka Uchida,Katharina M Michalik,Benjamin Meder,Hugo A Katus,Jan Haas,Wei Chen,Soni S Pullamsetti,Werner Seeger,Andreas M Zeiher,Stefanie Dimmeler,Christoph M Zehendner

PloS one 9:e94282 PubMed24728308

2014

B Lymphocyte Stimulator (BLyS) is expressed in human adipocytes in vivo and is related to obesity but not to insulin resistance.

Applications

WB

Species

Human

Nike Müller,Dominik M Schulte,Susann Hillebrand,Kathrin Türk,Jochen Hampe,Clemens Schafmayer,Mario Brosch,Witigo von Schönfels,Markus Ahrens,Rainald Zeuner,Johann O Schröder,Matthias Blüher,Christian Gutschow,Sandra Freitag-Wolf,Marta Stelmach-Mardas,Carina Saggau,Stefan Schreiber,Matthias Laudes
View all publications
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Product promise

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