AB7008

Donkey anti-Rat IgG H&L (PE)

Name: Donkey F(ab')2 Anti Rat (IgG) secondary antibody preadsorbed PE (ab7008) | Abcam
Brand: Abcam Limited
SKU: AB7008
Price: 700 USD
Availability: InStock
Rating: 4 (1 reviews)

(1 Review)

(0 Publication)

Suitable for Flow Cyt, IM, IHC-Fr, IHC-P, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining.

View Alternative Names

Ig gamma-1 chain C region

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey anti-Rat IgG H&L (PE) (AB7008)

ICC/IF

AbReview

Immunocytochemistry/ Immunofluorescence - Donkey anti-Rat IgG H&L (PE) (AB7008)

Immunocytochemsity/Immunofluorescence analysis of lung tissue using Donkey anti-rabbit IgG H&L (PE) secondary antibody (ab7008).

This image is courtesy of an anonymous Abreview

Flow Cytometry - Donkey anti-Rat IgG H&L (PE) (AB7008)

Flow Cyt

Unknown

Flow Cytometry - Donkey anti-Rat IgG H&L (PE) (AB7008)

ab7008 was used with the primary antibody ab19765 in Flow Cyt. See Abreview on ab19765.

Unconjugated

Donkey Anti-Rat IgG H&L
615 Texas Red®

Donkey Anti-Rat IgG H&L (Texas Red ®)
519 FITC

Donkey Anti-Rat IgG H&L (FITC)
518 DyLight® 488

Donkey Anti-Rat IgG H&L (DyLight® 488) preadsorbed
Biotin

Donkey Anti-Rat IgG H&L (Biotin) preadsorbed
673 DyLight® 650

Donkey Anti-Rat IgG H&L (DyLight® 650) preadsorbed
HRP

Donkey Anti-Rat IgG H&L (HRP) preadsorbed
617 Alexa Fluor® 594

Donkey Anti-Rat IgG H&L (Alexa Fluor® 594) preadsorbed
702 Alexa Fluor® 680

Donkey Anti-Rat IgG H&L (Alexa Fluor® 680) preadsorbed
603 Alexa Fluor® 568

Donkey Anti-Rat IgG H&L (Alexa Fluor® 568) preadsorbed
775 Alexa Fluor® 750

Donkey Anti-Rat IgG H&L (Alexa Fluor® 750) preadsorbed
519 Alexa Fluor® 488

Donkey Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed
665 Alexa Fluor® 647

Donkey Anti-Rat IgG H&L (Alexa Fluor® 647) preadsorbed
576 DyLight® 550

Donkey Anti-Rat IgG H&L (DyLight® 550) preadsorbed
565 Alexa Fluor® 555

Donkey Anti-Rat IgG H&L (Alexa Fluor® 555) preadsorbed
421 Alexa Fluor® 405

Donkey Anti-Rat IgG H&L (Alexa Fluor® 405) preadsorbed

Key facts

Host species

Donkey

Target species

Rat

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human

Pre-adsorbed

Yes

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Applications

IHC-Fr, IHC-P, Flow Cyt, ICC/IF, IM

applications

Clonality

Polyclonal

Specificity

No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Sheep Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/250", "notes":"The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining." }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/50 - 1/200", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/50 - 1/200", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/250", "notes":"" } } }

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Product details

Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey anti-Rat IgG H&L (PE)

Immunocytochemistry/ Immunofluorescence - Donkey anti-Rat IgG H&L (PE) (AB7008)

Flow Cytometry - Donkey anti-Rat IgG H&L (PE) (AB7008)

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Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Specificity

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise