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Suitable for Flow Cyt, IM, IHC-Fr, IHC-P, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining.
Alternative names=Ig gamma-1 chain C region
- Host species
Donkey
- Target species
Rat
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Minimal cross-reactivity
Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Applications
Flow Cyt, IM, IHC-Fr, IHC-P, ICC/IF
- Clonality
Polyclonal
- Format
F(ab')2
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info 1/100 - 1/250 | Notes The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. |
Application IM | Reactivity Reacts | Dilution info 1/50 - 1/200 | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info 1/50 - 1/200 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/50 - 1/200 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/100 - 1/250 | Notes - |
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Suitable for Flow Cyt, IM, IHC-Fr, IHC-P, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining.
Alternative names=Ig gamma-1 chain C region
Key facts
- Description
Donkey anti-Rat IgG H&L (PE)
- Host species
Donkey
- Target species
Rat
- Target isotype
IgG
- Target specificity
Heavy & Light chains
- Minimal cross-reactivity
Mouse, Sheep, Rabbit, Goat, Horse, Chicken, Guinea pig, Hamster, Cow, Human
- Conjugation
PE
- Excitation/Emission
Ex: 480;565nm, Em: 578nm
- Applications
Flow Cyt, IM, IHC-Fr, IHC-P, ICC/IF
- Clonality
Polyclonal
- Format
F(ab')2
- Pre-adsorbed
Yes
- Specificity
No reaction was observed against anti-Pepsin, anti-Donkey IgG F(c), or Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit or Sheep Serum Proteins.
- Concentration
Properties
- Form
Liquid
- Storage buffer
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.878% Sodium chloride, 0.424% Tripotassium orthophosphate- Purification technique
Affinity purification Immunogen
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
Notes
Can be used in immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
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