AB6895

Donkey Anti-Sheep IgG H&L

Name: Donkey Anti-Sheep IgG H&L secondary antibody (ab6895) | Abcam
Brand: Abcam Limited
SKU: AB6895
Price: 220 USD
Availability: InStock

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(2 Publications)

Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF. Cited in 2 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

1 Images

ELISA - Donkey Anti-Sheep IgG H&L (AB6895)

ELISA

Supplier Data

ELISA - Donkey Anti-Sheep IgG H&L (AB6895)

ELISA results of ab6895 against purified Sheep IgG. Each well was coated in duplicate with 1.0 µg of Sheep IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Gt-a-Donkey IgG HRP conjugate at 1/10,000 and TMB-8000 substrate.

Key facts

Host species

Donkey

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Applications

WB, Conjugation, ELISA, IP, IHC-P, IHC-Fr, ID, ICC/IF

applications

Clonality

Polyclonal

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Conjugation": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IP": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ID": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/2000 - 1/10000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/100000", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Anti-SHEEP IgG Antibody was prepared from monospecific antiserum generated in donkey by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Sheep IgG

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroscience research 93:644-50 PubMed25476208

2014

Vasoactive Intestinal peptide modulates c-Fos activity in the trigeminal nucleus and dura mater mast cells in sympathectomized rats.

Applications

Unspecified application

Species

Unspecified reactive species

Erkan Kilinc,Tülin Firat,Fatma Tore,Aysu Kiyan,Aysel Kukner,Nese Tunçel

Cerebral cortex (New York, N.Y. : 1991) 19:1738-50 PubMed19008461

2008

Novel markers reveal subpopulations of subplate neurons in the murine cerebral cortex.

Applications

IHC

Species

Unspecified reactive species

Anna Hoerder-Suabedissen,Wei Zhi Wang,Sheena Lee,Kay E Davies,André M Goffinet,Sonja Rakić,John Parnavelas,Kerstin Reim,Margareta Nicolić,Ole Paulsen,Zoltán Molnár

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Sheep IgG H&L

ELISA - Donkey Anti-Sheep IgG H&L (AB6895)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Applications

Clonality

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (2)

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Product promise