Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF. Cited in 2 publications.
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Publications
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- Journal of neuroscience research 93:644-502014Vasoactive Intestinal peptide modulates c-Fos activity in the trigeminal nucleus and dura mater mast cells in sympathectomized rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesErkan Kilinc,Tülin Firat,Fatma Tore,Aysu Kiyan,Aysel Kukner,Nese TunçelPubMed 25476208
- Cerebral cortex (New York, N.Y. : 1991) 19:1738-502008Novel markers reveal subpopulations of subplate neurons in the murine cerebral cortex.Applications:IHCReactive species:Unspecified reactive speciesAnna Hoerder-Suabedissen,Wei Zhi Wang,Sheena Lee,Kay E Davies,André M Goffinet,Sonja Rakić,John Parnavelas,Kerstin Reim,Margareta Nicolić,Ole Paulsen,Zoltán MolnárPubMed 19008461
Key facts
- Host species
- Donkey
- Target species
- Sheep
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Applications
- Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Conjugation | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IP | Reactivity Expected | Dilution info - | Notes - |
Application ID | Reactivity Expected | Dilution info - | Notes - |
Application WB | Reactivity Expected | Dilution info 1/2000 - 1/10000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/20000 - 1/100000 | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/1000 - 1/5000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF. Cited in 2 publications.
Key facts
- Description
- Donkey Anti-Sheep IgG H&L
- Host species
- Donkey
- Target species
- Sheep
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Applications
- Conjugation, IHC-Fr, IHC-P, IP, ID, WB, ELISA, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
Anti-SHEEP IgG Antibody was prepared from monospecific antiserum generated in donkey by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
ELISA - Donkey Anti-Sheep IgG H&L (ab6895)
ELISA results of ab6895 against purified Sheep IgG. Each well was coated in duplicate with 1.0 µg of Sheep IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Gt-a-Donkey IgG HRP conjugate at 1/10,000 and TMB-8000 substrate.
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