Donkey Anti-Sheep IgG H&L (Alexa Fluor® 405)
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(9 Publications)
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 405) (AB175676)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab7291, 1μg/ml) overnight at +4°C. ab6710, Sheep Anti-Mouse IgG H&L, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab175676 Alexa Fluor® 405 Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (blue) was then used at 1μg/ml for 1h as a tertiary antibody. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
- Alexa Fluor®
Unknown
Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 405) (AB175676)
Reactivity data
Product details
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Publications (9)
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Nature cell biology 26:698-709 PubMed38548890
2024
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The Journal of cell biology 222: PubMed37909920
2023
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International journal of molecular sciences 23: PubMed36233357
2022
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iScience 25:105308 PubMed36388952
2022
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Molecular biology of the cell 33:ar123 PubMed36044338
2022
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iScience 25:104803 PubMed35992071
2022
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Genes 12: PubMed34946963
2021
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The Journal of comparative neurology 529:1273-1292 PubMed32869307
2020
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Nature neuroscience 19:888-96 PubMed27214567
2016
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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