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AB175676

Donkey Anti-Sheep IgG H&L (Alexa Fluor® 405)

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(9 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 9 publications.
2 Images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 405) (AB175676)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 405) (AB175676)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab7291, 1μg/ml) overnight at +4°C. ab6710, Sheep Anti-Mouse IgG H&L, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab175676 Alexa Fluor® 405 Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (blue) was then used at 1μg/ml for 1h as a tertiary antibody. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 405) (AB175676)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 405) (AB175676)

Key facts

Host species

Donkey

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

ICC/IF, IHC-P, IHC-Fr, ELISA, Flow Cyt

applications

Clonality

Polyclonal

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p>We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor<sup>®</sup> 405 dye (see difference below)<br>Ex max: Alexa Fluor<sup>®</sup> 405 = 402nm / DAPI = 359nm<br>Em max: Alexa Fluor<sup>®</sup> 405 = 421nm / DAPI = 461nm</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }

Product details

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 26:698-709 PubMed38548890

2024

A cell fate decision map reveals abundant direct neurogenesis bypassing intermediate progenitors in the human developing neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Laure Coquand,Clarisse Brunet Avalos,Anne-Sophie Macé,Sarah Farcy,Amandine Di Cicco,Marusa Lampic,Ryszard Wimmer,Betina Bessières,Tania Attie-Bitach,Vincent Fraisier,Pierre Sens,Fabien Guimiot,Jean-Baptiste Brault,Alexandre D Baffet

The Journal of cell biology 222: PubMed37909920

2023

Axonal transport of autophagosomes is regulated by dynein activators JIP3/JIP4 and ARF/RAB GTPases.

Applications

Unspecified application

Species

Unspecified reactive species

Sydney E Cason,Erika L F Holzbaur

International journal of molecular sciences 23: PubMed36233357

2022

Decreased Prosaposin and Progranulin in the Cingulate Cortex Are Associated with Schizophrenia Pathophysiology.

Applications

Unspecified application

Species

Unspecified reactive species

Yachao He,Xiaoqun Zhang,Ivana Flais,Per Svenningsson

iScience 25:105308 PubMed36388952

2022

Transplanted human induced pluripotent stem cells- derived retinal ganglion cells embed within mouse retinas and are electrophysiologically functional.

Applications

Unspecified application

Species

Unspecified reactive species

Vrathasha Vrathasha,Sergei Nikonov,Brent Allen Bell,Jie He,Yajat Bungatavula,Katherine Elizabeth Uyhazi,Venkata Ramana Murthy Chavali

Molecular biology of the cell 33:ar123 PubMed36044338

2022

Spatiotemporal analysis of axonal autophagosome-lysosome dynamics reveals limited fusion events and slow maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Sydney E Cason,Saurabh S Mogre,Erika L F Holzbaur,Elena F Koslover

iScience 25:104803 PubMed35992071

2022

Cochlear ribbon synapse maturation requires Nlgn1 and Nlgn3.

Applications

Unspecified application

Species

Unspecified reactive species

Miguel A Ramirez,Yuzuru Ninoyu,Cayla Miller,Leonardo R Andrade,Seby Edassery,Ewa Bomba-Warczak,Briana Ortega,Uri Manor,Mark A Rutherford,Rick A Friedman,Jeffrey N Savas

Genes 12: PubMed34946963

2021

Single Cell Sequencing of Induced Pluripotent Stem Cell Derived Retinal Ganglion Cells (iPSC-RGC) Reveals Distinct Molecular Signatures and RGC Subtypes.

Applications

Unspecified application

Species

Unspecified reactive species

Harini V Gudiseva,Vrathasha Vrathasha,Jie He,Devesh Bungatavula,Joan M O'Brien,Venkata R M Chavali

The Journal of comparative neurology 529:1273-1292 PubMed32869307

2020

Heterogeneous expression of dopaminergic markers and Vglut2 in mouse mesodiencephalic dopaminergic nuclei A8-A13.

Applications

Unspecified application

Species

Unspecified reactive species

Maxime Fougère,Cornelis Immanuel van der Zouwen,Joël Boutin,Dimitri Ryczko

Nature neuroscience 19:888-96 PubMed27214567

2016

Hedgehog signaling promotes basal progenitor expansion and the growth and folding of the neocortex.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Wang,Shirui Hou,Young-Goo Han
View all publications

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