Donkey Anti-Sheep IgG H&L (Alexa Fluor® 568)
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(12 Publications)
- ICC/IF
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Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 568) (AB175712)
ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. ab6710, sheep anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab175712, Donkey Anti-Sheep IgG H&L (Alexa Fluor® 568) (yellow) was then used at 1μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
Alexa Fluor® 568 is pseudocolored in yellow.
The secondary antibody only control displays negative staining, as expected.
- Alexa Fluor®
Unknown
Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 568) (AB175712)
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Publications (12)
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Hippocampus 34:156-165 PubMed38100162
2023
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Cell reports 42:113423 PubMed37952151
2023
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Biophysical reports 3:100091 PubMed36619899
2023
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International journal of molecular sciences 23: PubMed36362165
2022
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International journal of molecular sciences 23: PubMed36232822
2022
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Brain sciences 12: PubMed36138975
2022
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Nature protocols 17:890-909 PubMed35140411
2022
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Progress in biophysics and molecular biology 168:3-9 PubMed34536443
2021
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Biomedical optics express 12:3684-3699 PubMed34221688
2021
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Scientific reports 10:1300 PubMed31992767
2020
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