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AB175712

Donkey Anti-Sheep IgG H&L (Alexa Fluor® 568)

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(12 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 12 publications.
2 Images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 568) (AB175712)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 568) (AB175712)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1μg/ml) overnight at +4°C. ab6710, sheep anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab175712, Donkey Anti-Sheep IgG H&L (Alexa Fluor® 568) (yellow) was then used at 1μg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® 568 is pseudocolored in yellow.

The secondary antibody only control displays negative staining, as expected.

Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 568) (AB175712)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 568) (AB175712)

Key facts

Host species

Donkey

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Applications

Flow Cyt, ICC/IF, ELISA, IHC-Fr, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Hippocampus 34:156-165 PubMed38100162

2023

Hippocampal parvalbumin and perineuronal nets: Possible involvement in anxiety-like behavior in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Zhixin Fan,Xiayu Gong,Hanfang Xu,Yue Qu,Bozhi Li,Lanxin Li,Yuqi Yan,Lili Wu,Can Yan

Cell reports 42:113423 PubMed37952151

2023

Jedi-1/MEGF12-mediated phagocytosis controls the pro-neurogenic properties of microglia in the ventricular-subventricular zone.

Applications

Unspecified application

Species

Unspecified reactive species

Vivianne Morrison,Matthew Houpert,Jonathan Trapani,Asa Brockman,Philip Kingsley,Ketaki Katdare,Hillary Layden,Gabriela Nguena-Jones,Alexandra Trevisan,Kathleen Maguire-Zeiss,Lawrence Marnett,Gregory Bix,Rebecca Ihrie,Bruce Carter

Biophysical reports 3:100091 PubMed36619899

2023

Expansion microscopy of neutrophil nuclear structure and extracellular traps.

Applications

Unspecified application

Species

Unspecified reactive species

Jason Scott Holsapple,Lena Schnitzler,Louisa Rusch,Tobias Horst Baldeweg,Elsa Neubert,Sebastian Kruss,Luise Erpenbeck

International journal of molecular sciences 23: PubMed36362165

2022

Immunothrombotic Mechanisms Induced by Ingenol Mebutate Lead to Rapid Necrosis and Clearance of Anogenital Warts.

Applications

Unspecified application

Species

Unspecified reactive species

Stephan A Braun,Alexander T Bauer,Csongor Németh,Annamária Rózsa,Louisa Rusch,Luise Erpenbeck,Sebastian Schloer,Steffi Silling,Dieter Metze,Peter A Gerber,Stefan W Schneider,Rolland Gyulai,Bernhard Homey

International journal of molecular sciences 23: PubMed36232822

2022

Directional Submicrofiber Hydrogel Composite Scaffolds Supporting Neuron Differentiation and Enabling Neurite Alignment.

Applications

Unspecified application

Species

Unspecified reactive species

Lena Mungenast,Fabian Züger,Jasmin Selvi,Ana Bela Faia-Torres,Jürgen Rühe,Laura Suter-Dick,Maurizio R Gullo

Brain sciences 12: PubMed36138975

2022

Experimental Induction of Intracranial Aneurysms in Rats: A New Model Utilizing a Genetic Modification within the EDNRA Gene.

Applications

Unspecified application

Species

Unspecified reactive species

Tim Lampmann,Valeri Borger,Jürgen Konczalla,Suzana Gispert,Georg Auburger,Hartmut Vatter,Erdem Güresir

Nature protocols 17:890-909 PubMed35140411

2022

Spotting-based differentiation of functional dopaminergic progenitors from human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jisun Kim,Jeha Jeon,Bin Song,Nayeon Lee,Sanghyeok Ko,Young Cha,Pierre Leblanc,Hyemyung Seo,Kwang-Soo Kim

Progress in biophysics and molecular biology 168:3-9 PubMed34536443

2021

Exploring the human cerebral cortex using confocal microscopy.

Applications

Unspecified application

Species

Unspecified reactive species

Luca Pesce,Annunziatina Laurino,Marina Scardigli,Jiarui Yang,David A Boas,Patrick R Hof,Christophe Destrieux,Irene Costantini,Francesco Saverio Pavone

Biomedical optics express 12:3684-3699 PubMed34221688

2021

Large-scale, cell-resolution volumetric mapping allows layer-specific investigation of human brain cytoarchitecture.

Applications

Unspecified application

Species

Unspecified reactive species

Irene Costantini,Giacomo Mazzamuto,Matteo Roffilli,Annunziatina Laurino,Filippo Maria Castelli,Mattia Neri,Giovanni Lughi,Andrea Simonetto,Erica Lazzeri,Luca Pesce,Christophe Destrieux,Ludovico Silvestri,Valerio Conti,Renzo Guerrini,Francesco Saverio Pavone

Scientific reports 10:1300 PubMed31992767

2020

Jedi-1 deficiency increases sensory neuron excitability through a non-cell autonomous mechanism.

Applications

Unspecified application

Species

Unspecified reactive species

Alexandra J Trevisan,Mary Beth Bauer,Rebecca L Brindley,Kevin P M Currie,Bruce D Carter
View all publications

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