AB150180

Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594)

Name: Donkey Anti Sheep (IgG) secondary antibody Alexa Fluor® 594 (ab150180) | Abcam
Brand: Abcam Limited
SKU: AB150180
Price: 165 USD
Availability: InStock

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(14 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 14 publications.

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594) (AB150180)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594) (AB150180)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 2µg/ml) overnight at +4°C. ab6710, sheep anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150180 Alexa Fluor® 594 donkey anti-sheep IgG (H+L) (orange) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594) (AB150180)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594) (AB150180)

Key facts

Host species

Donkey

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 594

Excitation/Emission

Ex: 590nm, Em: 617nm

Applications

Flow Cyt, ICC/IF, ELISA, IHC-P, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

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Product details

This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Sheep IgG

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Publications (14)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40362219

2025

Adult Neurogenesis Is Regulated by the Endocannabinoid and Kisspeptin Systems.

Applications

Unspecified application

Species

Unspecified reactive species

Marianna Marino,Paola Di Pietro,Raffaella D'Auria,Martina Lombardi,Grazia Maria Giovanna Pastorino,Jacopo Troisi,Francesca Felicia Operto,Albino Carrizzo,Carmine Vecchione,Andrea Viggiano,Rosaria Meccariello,Antonietta Santoro

iScience 28:111994 PubMed40071147

2025

IQGAP2 regulates blood-brain barrier immune dynamics.

Applications

Unspecified application

Species

Unspecified reactive species

Ketaki A Katdare,Andrew Kjar,Natasha M O'Brown,Emma H Neal,Alexander G Sorets,Alena Shostak,Wilber Romero-Fernandez,Alexander J Kwiatkowski,Kate Mlouk,Hyosung Kim,Rebecca P Cowell,Katrina R Schwensen,Cristian O Carvajal Tapia,Guste Venslovaite,Kensley B Horner,John T Wilson,Matthew S Schrag,Sean G Megason,Ethan S Lippmann

Developmental dynamics : an official publication of the American Association of Anatomists 254:1040-1054 PubMed39853824

2025

Endocytosis mediated by megalin and cubilin is involved in enamel development.

Applications

Unspecified application

Species

Unspecified reactive species

Aijia Wang,Yangxi Chen,Xinye Zhang,Ming Liu,Shumin Liu,Renata Kozyraki,Zhi Chen

Genetics 226: PubMed37816306

2023

Isolated loss of the AUTS2 long isoform, brain-wide or targeted to Calbindin-lineage cells, generates a specific suite of brain, behavioral, and molecular pathologies.

Applications

Unspecified application

Species

Unspecified reactive species

Yunshu Song,Christopher H Seward,Chih-Ying Chen,Amber LeBlanc,Analise M Leddy,Lisa Stubbs

NPJ vaccines 8:132 PubMed37679361

2023

A mosaic influenza virus-like particles vaccine provides broad humoral and cellular immune responses against influenza A viruses.

Applications

Unspecified application

Species

Unspecified reactive species

Xuejie Liu,Tianyi Zhao,Liangliang Wang,Zhuolin Yang,Chuming Luo,Minchao Li,Huanle Luo,Caijun Sun,Huacheng Yan,Yuelong Shu

Acta pharmacologica Sinica 44:513-523 PubMed36100766

2022

Humanized cerebral organoids-based ischemic stroke model for discovering of potential anti-stroke agents.

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Na Wang,Zhi Wang,Xi-Yuan Wang,Xiu-Ping Zhang,Tian-Ying Xu,Chao-Yu Miao

Nature communications 12:4645 PubMed34330918

2021

Identification of the cross-strand chimeric RNAs generated by fusions of bi-directional transcripts.

Applications

Unspecified application

Species

Unspecified reactive species

Yuting Wang,Qin Zou,Fajin Li,Wenwei Zhao,Hui Xu,Wenhao Zhang,Haiteng Deng,Xuerui Yang

Cell transplantation 29:963689720927392 PubMed32584149

2020

Long-Term Liraglutide Administration Induces Pancreas Neogenesis in Adult T2DM Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Hongjun Deng,Fengying Yang,Xiaoyi Ma,Ying Wang,Qi Chen,Li Yuan

Neurospine 17:69-76 PubMed32252156

2020

Rabbit Annulus Fibrosus Cells Express Neuropeptide Y, Which Is Influenced by Mechanical and Inflammatory Stress.

Applications

Unspecified application

Species

Unspecified reactive species

Malcolm E Dombrowski,Adam S Olsen,Nicholas Vaudreuil,Brandon K Couch,Qing Dong,Michelle Tucci,Joon Y Lee,Nam V Vo,Gwendolyn Sowa

Cancers 11: PubMed31600958

2019

Tumor Drug Distribution after Local Drug Delivery by Hyperthermia, In Vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Helena C Besse,Angelique D Barten-van Rijbroek,Kim M G van der Wurff-Jacobs,Clemens Bos,Chrit T W Moonen,Roel Deckers

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594) (AB150180)

Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594) (AB150180)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (14)

Product promise