Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 9 publications.
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Publications
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- NPJ vaccines 8:1322023A mosaic influenza virus-like particles vaccine provides broad humoral and cellular immune responses against influenza A viruses.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXuejie Liu,Tianyi Zhao,Liangliang Wang,Zhuolin Yang,Chuming Luo,Minchao Li,Huanle Luo,Caijun Sun,Huacheng Yan,Yuelong ShuPubMed 37679361
- Acta pharmacologica Sinica 44:513-5232022Humanized cerebral organoids-based ischemic stroke model for discovering of potential anti-stroke agents.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShu-Na Wang,Zhi Wang,Xi-Yuan Wang,Xiu-Ping Zhang,Tian-Ying Xu,Chao-Yu MiaoPubMed 36100766
- Nature communications 12:46452021Identification of the cross-strand chimeric RNAs generated by fusions of bi-directional transcripts.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuting Wang,Qin Zou,Fajin Li,Wenwei Zhao,Hui Xu,Wenhao Zhang,Haiteng Deng,Xuerui YangPubMed 34330918
- Neurospine 17:69-762020Rabbit Annulus Fibrosus Cells Express Neuropeptide Y, Which Is Influenced by Mechanical and Inflammatory Stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMalcolm E Dombrowski et. al.PubMed 32252156
- Cancers 11:2019Tumor Drug Distribution after Local Drug Delivery by Hyperthermia, In Vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHelena C Besse et. al.PubMed 31600958
- Brain structure & function 224:2247-22672019On the existence of mechanoreceptors within the neurovascular unit of the mammalian brain.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJorge Larriva-Sahd et. al.PubMed 31190162
- The Journal of comparative neurology 526:2019-20312018c-Fos marking of identified midbrain neurons coactive after nicotine administration in-vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatja Baur,Arian Hach,Rick E Bernardi,Rainer Spanagel,Hilmar Bading,C Peter BengtsonPubMed 29888787
- The Journal of clinical investigation 127:1475-14842017Loss of DDRGK1 modulates SOX9 ubiquitination in spondyloepimetaphyseal dysplasia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdetutu T Egunsola et. al.PubMed 28263186
- Endocrinology 157:1071-812016Intestinal Bile Acid Composition Modulates Prohormone Convertase 1/3 (PC1/3) Expression and Consequent GLP-1 Production in Male Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKohkichi Morimoto et. al.PubMed 26789236
Key facts
- Host species
- Donkey
- Target species
- Sheep
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 594
- Excitation/Emission
- Ex: 590nm, Em: 617nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 9 publications.
Key facts
- Description
- Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594)
- Host species
- Donkey
- Target species
- Sheep
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 594
- Excitation/Emission
- Ex: 590nm, Em: 617nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This product is provided under an intellectual property license from Life Technologies Corporation. The purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: (i) in manufacturing; (ii) to provide a service, information, or data in return for payment; (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are resold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594) (ab150180)
ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 2µg/ml) overnight at +4°C. Sheep Anti-Mouse IgG H&L ab6710, sheep anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150180 Alexa Fluor® 594 donkey anti-sheep IgG (H+L) (orange) was then used at 1µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 594) (ab150180)
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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