AB150179

Donkey Anti-Sheep IgG H&L (Alexa Fluor® 647)

Name: Donkey Anti-Sheep IgG (Alexa Fluor® 647) secondary antibody (ab150179) | Abcam
Brand: Abcam Limited
SKU: AB150179
Price: 165 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(48 Publications)

Donkey Anti-Sheep IgG H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.

- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 20 publications

2 Images

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 647) (AB150179)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 647) (AB150179)

ICC/IF image of ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab7291, 1µg/ml) overnight at +4°C. ab6710, sheep anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150179 Alexa Fluor® 647 donkey anti-sheep IgG (H+L) (red) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 647) (AB150179)

Alexa Fluor®

Unknown

Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 647) (AB150179)

Emission Spectra of the Alexa Fluor^® Conjugated Secondary Antibodies.

Key facts

Host species

Donkey

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

ELISA, ICC/IF, IHC-Fr, IHC-P, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"" } } }

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Product details

This antibody reacts with the heavy and light chains of Sheep IgG

Fluorochrome chart- a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies, designed to deliver precise and reliable results.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.

Storage buffer

Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Sheep IgG

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Publications (48)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 26: PubMed40869287

2025

Differential Sensitivity of Hippocampal GABAergic Neurons to Hypoxia and Ischemia-like Conditions Correlates with the Type of Calcium-Binding Protein Expressed.

Applications

Unspecified application

Species

Unspecified reactive species

Maria V Turovskaya,Valery P Zinchenko

eNeuro 12: PubMed40695594

2025

Altered Cerebral Cortical Gyrification in Ferrets with Neonatal Exposure to the Bacterial Endotoxin, Lipopolysaccharide.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuhiko Sawada,Rie Ryoke,Hiroi Nonaka,Ryuta Kawashima,Akira Sumiyoshi

Cancer immunology research 13:729-748 PubMed40249209

2025

Antitumor CD4+ T Helper 1 Cells Target and Control the Outgrowth of Disseminated Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Ganesan Ramamoorthi,Marie Catherine Lee,Carly M Farrell,Colin Snyder,Saurabh K Garg,Amy L Aldrich,Vincent Lok,William Dominguez-Viqueira,Sy K Olson-Mcpeek,Marilin Rosa,Namrata Gautam,Shari Pilon-Thomas,Ling Cen,Krithika N Kodumudi,Doris Wiener,Thordur Oskarsson,Ana P Gomes,Robert A Gatenby,Brian J Czerniecki

Nature communications 16:2845 PubMed40122897

2025

Astrocyte-secreted cues promote neural maturation and augment activity in human forebrain organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Honghui Zheng,Yilin Feng,Jiyuan Tang,Feifei Yu,Zitian Wang,Jiani Xu,Cheng Hai,Mingyue Jiang,Yifan Cheng,Zhicheng Shao,Ning Ma,Peter E Lobie,Shaohua Ma

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2410927 PubMed39435757

2024

Enriched Environment Reduces Seizure Susceptibility via Entorhinal Cortex Circuit Augmented Adult Neurogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Zhongxia Li,Liying Chen,Fan Fei,Wenqi Wang,Lin Yang,Yu Wang,Heming Cheng,Yingwei Xu,Cenglin Xu,Shuang Wang,Yan Gu,Feng Han,Zhong Chen,Yi Wang

eLife 13: PubMed39361026

2024

An in vitro approach reveals molecular mechanisms underlying endocrine disruptor-induced epimutagenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Jake D Lehle,Yu-Huey Lin,Amanda Gomez,Laura Chavez,John R McCarrey

Nature communications 15:6773 PubMed39117691

2024

Circular RNAs regulate neuron size and migration of midbrain dopamine neurons during development.

Applications

Unspecified application

Species

Unspecified reactive species

Mateja Rybiczka-Tešulov,Oxana Garritsen,Morten T Venø,Laura Wieg,Roland van Dijk,Karim Rahimi,Andreia Gomes-Duarte,Marina de Wit,Lieke L van de Haar,Lars Michels,Nicky C H van Kronenburg,Christiaan van der Meer,Jørgen Kjems,Vamshidhar R Vangoor,R Jeroen Pasterkamp

Alzheimer's research & therapy 16:121 PubMed38831312

2024

Intermittent hypoxia training enhances Aβ endocytosis by plaque associated microglia via VPS35-dependent TREM2 recycling in murine Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xueting Wang,Yuqi Xie,Xiaoyang Fan,Xiaomei Wu,Dan Wang,Li Zhu

Nature communications 15:3946 PubMed38729950

2024

Multiplexed bulk and single-cell RNA-seq hybrid enables cost-efficient disease modeling with chimeric organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Chen Cheng,Gang Wang,Yuqing Zhu,Hangdi Wu,Li Zhang,Zhihong Liu,Yuanhua Huang,Jin Zhang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2402059 PubMed38704728

2024

Impaired Meningeal Lymphatics and Glymphatic Pathway in Patients with White Matter Hyperintensity.

Applications

Unspecified application

Species

Unspecified reactive species

Ying Zhou,Rui Xue,Yifei Li,Wang Ran,Yuping Chen,Zhongyu Luo,Kemeng Zhang,Ruoxia Zhang,Junjun Wang,Mengmeng Fang,Cong Chen,Min Lou

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Sheep IgG H&L (Alexa Fluor® 647)

Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 647) (AB150179)

Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 647) (AB150179)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (48)

Product promise