Donkey Anti-Sheep IgG H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 20 publications
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- Biotechnology and bioengineering 121:1435-14522024Mechanobiological stimulation in organ-on-a-chip systems reduces hepatic drug metabolic capacity in favor of regenerative specialization.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenedikt Scheidecker,Stéphane Poulain,Masahiro Sugimoto,Hiroshi Arakawa,Soo H Kim,Takumi Kawanishi,Yukio Kato,Mathieu Danoy,Masaki Nishikawa,Yasuyuki SakaiPubMed 38184801
- iScience 27:1085802024FLOT2 promotes nasopharyngeal carcinoma progression through suppression of TGF-β pathway via facilitating CD109 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongjuan Xu,Yuze Yin,Yihan Li,Ning Shi,Wen Xie,Weiren Luo,Lei Wang,Bin Zhu,Weidong Liu,Xingjun Jiang,Caiping RenPubMed 38161417
- Nature communications 14:71472023Pathogenesis-adaptive polydopamine nanosystem for sequential therapy of ischemic stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDi Wu,Jing Zhou,Yanrong Zheng,Yuyi Zheng,Qi Zhang,Zhuchen Zhou,Xiaojie Chen,Qi Chen,Yeping Ruan,Yi Wang,Zhong ChenPubMed 37932306
- Frontiers in immunology 14:12268322023Complement inhibition can decrease the haemostatic response in a microvascular bleeding model at multiple levels.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMurielle Golomingi,Jessie Kohler,Christina Lamers,Richard B Pouw,Daniel Ricklin,József Dobó,Péter Gál,Gábor Pál,Bence Kiss,Arthur Dopler,Christoph Q Schmidt,Elaissa Trybus Hardy,Wilbur Lam,Verena SchroederPubMed 37771595
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e23032912023Lepr-Expressing PDLSCs Contribute to Periodontal Homeostasis and Respond to Mechanical Force by Piezo1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDanting Zhang,Weimin Lin,Shuang Jiang,Peng Deng,Linfeng Liu,Qian Wang,Rui Sheng,Hui Sophie Shu,Lijun Wang,Weiguo Zou,Bo O Zhou,Junjun Jing,Ling Ye,Bo Yu,Shiwen Zhang,Quan YuanPubMed 37553778
- Cell reports 41:1116862022Plaque contact and unimpaired Trem2 is required for the microglial response to amyloid pathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJack I Wood,Eugenia Wong,Ridwaan Joghee,Aya Balbaa,Karina S Vitanova,Katie M Stringer,Alison Vanshoiack,Stefan-Laural J Phelan,Francesca Launchbury,Sneha Desai,Takshashila Tripathi,Jörg Hanrieder,Damian M Cummings,John Hardy,Frances A EdwardsPubMed 36417868
- American journal of physiology. Heart and circulatory physiology 323:H749-H7622022Adipose stromal vascular fraction reverses mitochondrial dysfunction and hyperfission in aging-induced coronary microvascular disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEvan Paul Tracy,Rajeev Nair,Gabrielle Rowe,Jason E Beare,Andreas Beyer,Amanda Jo LeBlancPubMed 36018760
- iScience 25:1048032022Cochlear ribbon synapse maturation requires Nlgn1 and Nlgn3.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMiguel A Ramirez,Yuzuru Ninoyu,Cayla Miller,Leonardo R Andrade,Seby Edassery,Ewa Bomba-Warczak,Briana Ortega,Uri Manor,Mark A Rutherford,Rick A Friedman,Jeffrey N SavasPubMed 35992071
- Advanced science (Weinheim, Baden-Wurttemberg, Germany) 9:e22022282022Activation of the Lateral Habenula-Ventral Tegmental Area Neural Circuit Contributes to Postoperative Cognitive Dysfunction in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJuan Xin,Weiran Shan,Jun Li,Hai Yu,Zhiyi ZuoPubMed 35616407
- eLife 11:2022Evolutionary conservation of centriole rotational asymmetry in the human centrosome.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNoémie Gaudin,Paula Martin Gil,Meriem Boumendjel,Dmitry Ershov,Catherine Pioche-Durieu,Manon Bouix,Quentin Delobelle,Lucia Maniscalco,Than Bich Ngan Phan,Vincent Heyer,Bernardo Reina-San-Martin,Juliette AzimzadehPubMed 35319462
Key facts
- Host species
- Donkey
- Target species
- Sheep
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
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Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Donkey Anti-Sheep IgG H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 20 publications
Key facts
- Description
- Donkey Anti-Sheep IgG H&L (Alexa Fluor® 647)
- Host species
- Donkey
- Target species
- Sheep
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This antibody reacts with the heavy and light chains of Sheep IgG
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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2 product images
Immunocytochemistry/ Immunofluorescence - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 647) (ab150179)
ICC/IF image of Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, 1µg/ml) overnight at +4°C. Sheep Anti-Mouse IgG H&L ab6710, sheep anti-mouse IgG, was then added as a secondary bridging antibody, at 1/250 dilution for 1h. ab150179 Alexa Fluor® 647 donkey anti-sheep IgG (H+L) (red) was then used at 2µg/ml for 1h as a tertiary antibody. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Alexa Fluor® - Donkey Anti-Sheep IgG H&L (Alexa Fluor® 647) (ab150179)
Emission Spectra of the Alexa Fluor® Conjugated Secondary Antibodies.
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