AB6901

Donkey Anti-Sheep IgG H&L (Alkaline Phosphatase)

Name: Donkey Anti Sheep (IgG) secondary antibody AP (ab6901) | Abcam
Brand: Abcam Limited
SKU: AB6901
Price: 265 USD
Availability: InStock

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(4 Publications)

Suitable for WB, Dot, IHC-Fr, IHC-P, IM, ELISA, ICC/IF. Cited in 4 publications.

1 Images

ELISA - Donkey Anti-Sheep IgG H&L (Alkaline Phosphatase) (AB6901)

ELISA

Supplier Data

ELISA - Donkey Anti-Sheep IgG H&L (Alkaline Phosphatase) (AB6901)

ELISA results of ab6901 against Sheep IgG. Each well was coated in 1.0 µg of antigen. The starting dilution of antibody was 5 µg/mL and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody.

Key facts

Host species

Donkey

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Alkaline Phosphatase

Excitation/Emission

Applications

ELISA, ICC/IF, WB, Dot, IHC-Fr, IHC-P, IM

applications

Clonality

Polyclonal

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/8000", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/200 - 1/1000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Anti-Sheep IgG (H&L) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

pH: 8 Preservative: 0.1% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Sheep IgG

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in bioengineering and biotechnology 10:917746 PubMed35845394

2022

Intensifying Continuous Production of Gag-HA VLPs at High Cell Density Using Stable Insect Cells Adapted to Low Culture Temperature.

Applications

Unspecified application

Species

Unspecified reactive species

Bárbara Fernandes,Ricardo Correia,Paula M Alves,António Roldão

International journal of molecular sciences 22: PubMed34205342

2021

Long-Term Effects of Repetitive Mild Traumatic Injury on the Visual System in Wild-Type and TDP-43 Transgenic Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Kristina Pilipović,Jelena Rajič Bumber,Petra Dolenec,Nika Gržeta,Tamara Janković,Jasna Križ,Gordana Župan

Biotechnology and bioengineering 118:2536-2547 PubMed33764532

2021

Integrating high cell density cultures with adapted laboratory evolution for improved Gag-HA virus-like particles production in stable insect cell lines.

Applications

Unspecified application

Species

Unspecified reactive species

Bárbara Fernandes,Ricardo Correia,Marcos Sousa,Manuel J T Carrondo,Paula M Alves,António Roldão

International journal of molecular sciences 20: PubMed30577572

2018

Mast Cell-Specific Expression of Human Siglec-8 in Conditional Knock-in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Yadong Wei,Krishan D Chhiba,Fengrui Zhang,Xujun Ye,Lihui Wang,Li Zhang,Piper A Robida,Liliana Moreno-Vinasco,Ronald L Schnaar,Axel Roers,Karin Hartmann,Chang-Min Lee,Delia Demers,Tao Zheng,Bruce S Bochner,Zhou Zhu

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Donkey Anti-Sheep IgG H&L (Alkaline Phosphatase)

ELISA - Donkey Anti-Sheep IgG H&L (Alkaline Phosphatase) (AB6901)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Complementary Products

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (4)

Product promise