AB6899

Donkey Anti-Sheep IgG H&L (Biotin)

Name: Donkey Anti Sheep (IgG) secondary antibody Biotin (ab6899) | Abcam
Brand: Abcam Limited
SKU: AB6899

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(6 Publications)

Suitable for Dot, IM, IHC-Fr, IHC-P, ELISA, WB, ICC/IF. Cited in 6 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

1 Images

ELISA - Donkey Anti-Sheep IgG H&L (Biotin) (AB6899)

ELISA

Supplier Data

ELISA - Donkey Anti-Sheep IgG H&L (Biotin) (AB6899)

ELISA results of ab6899 against Sheep IgG. Each well was coated in duplicate with 1.0 µg of Sheep IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Streptavidin HRP conjugate and TMB substrate.

Key facts

Host species

Donkey

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Biotin

Excitation/Emission

Applications

IHC-P, Dot, ICC/IF, IHC-Fr, ELISA, WB, IM

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/100000", "notes":"" }, "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/2000 - 1/10000", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

Anti-Sheep IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Sheep IgG

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 23: PubMed36361905

2022

Characterization of a Bioactive Peptide T14 in the Human and Rodent Substantia Nigra: Implications for Neurodegenerative Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Susan Adele Greenfield,Giovanni Ferrati,Clive W Coen,Auguste Vadisiute,Zoltan Molnár,Sara Garcia-Rates,Sally Frautschy,Gregory M Cole

Glia 67:741-758 PubMed30548340

2018

Astrocyte-targeted IL-10 production decreases proliferation and induces a downregulation of activated microglia/macrophages after PPT.

Applications

Unspecified application

Species

Unspecified reactive species

Mireia Recasens,Kalpana Shrivastava,Beatriz Almolda,Berta González,Bernardo Castellano

Experimental biology and medicine (Maywood, N.J.) 242:1499-1507 PubMed28766984

2017

Long-term effect of parasympathetic or sympathetic denervation on intestinal epithelial cell proliferation and apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

Elizabeth A Davis,Martha C Washington,Emily R Yaniz,Heidi Phillips,Ayman I Sayegh,Megan J Dailey

Experimental biology and medicine (Maywood, N.J.) 242:1490-1498 PubMed28766983

2017

LKB1-AMPK modulates nutrient-induced changes in the mode of division of intestinal epithelial crypt cells in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Katherine Blackmore,Weinan Zhou,Megan J Dailey

Glia 64:2104-2119 PubMed27535761

2016

Astrocyte-targeted production of interleukin-6 reduces astroglial and microglial activation in the cuprizone demyelination model: Implications for myelin clearance and oligodendrocyte maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Filip Petković,Iain L Campbell,Berta Gonzalez,Bernardo Castellano

The Journal of neuroscience : the official journal of the Society for Neuroscience 34:11984-2000 PubMed25186745

2014

Spinal cord injury enables aromatic L-amino acid decarboxylase cells to synthesize monoamines.

Applications

Unspecified application

Species

Unspecified reactive species

Jacob Wienecke,Li-Qun Ren,Hans Hultborn,Meng Chen,Morten Møller,Yifan Zhang,Mengliang Zhang

View all publications

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Product promise

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Donkey Anti-Sheep IgG H&L (Biotin)

ELISA - Donkey Anti-Sheep IgG H&L (Biotin) (AB6899)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Product protocols

Target data

Publications (6)

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise