Suitable for Dot, IM, IHC-Fr, IHC-P, ELISA, WB, ICC/IF. Cited in 6 publications.
Images
Publications
Filter by
- International journal of molecular sciences 23:2022Characterization of a Bioactive Peptide T14 in the Human and Rodent Substantia Nigra: Implications for Neurodegenerative Disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusan Adele Greenfield,Giovanni Ferrati,Clive W Coen,Auguste Vadisiute,Zoltan Molnár,Sara Garcia-Rates,Sally Frautschy,Gregory M ColePubMed 36361905
- Glia 67:741-7582018Astrocyte-targeted IL-10 production decreases proliferation and induces a downregulation of activated microglia/macrophages after PPT.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMireia Recasens et. al.PubMed 30548340
- Experimental biology and medicine (Maywood, N.J.) 242:1499-15072017Long-term effect of parasympathetic or sympathetic denervation on intestinal epithelial cell proliferation and apoptosis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElizabeth A Davis et. al.PubMed 28766984
- Experimental biology and medicine (Maywood, N.J.) 242:1490-14982017LKB1-AMPK modulates nutrient-induced changes in the mode of division of intestinal epithelial crypt cells in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatherine Blackmore et. al.PubMed 28766983
- Glia 64:2104-21192016Astrocyte-targeted production of interleukin-6 reduces astroglial and microglial activation in the cuprizone demyelination model: Implications for myelin clearance and oligodendrocyte maturation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesFilip Petković,Iain L Campbell,Berta Gonzalez,Bernardo CastellanoPubMed 27535761
- The Journal of neuroscience : the official journal of the Society for Neuroscience 34:11984-20002014Spinal cord injury enables aromatic L-amino acid decarboxylase cells to synthesize monoamines.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJacob Wienecke,Li-Qun Ren,Hans Hultborn,Meng Chen,Morten Møller,Yifan Zhang,Mengliang ZhangPubMed 25186745
Key facts
- Host species
- Donkey
- Target species
- Sheep
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Biotin
- Applications
- Dot, IM, IHC-Fr, IHC-P, ELISA, WB, ICC/IF
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Dot | Reactivity Expected | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info 1/1000 - 1/5000 | Notes - |
Application IHC-P | Reactivity Expected | Dilution info 1/1000 - 1/5000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/20000 - 1/100000 | Notes - |
Application WB | Reactivity Expected | Dilution info 1/2000 - 1/10000 | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/1000 - 1/5000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for Dot, IM, IHC-Fr, IHC-P, ELISA, WB, ICC/IF. Cited in 6 publications.
Key facts
- Description
- Donkey Anti-Sheep IgG H&L (Biotin)
- Host species
- Donkey
- Target species
- Sheep
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- Biotin
- Applications
- Dot, IM, IHC-Fr, IHC-P, ELISA, WB, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
Anti-Sheep IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
ELISA - Donkey Anti-Sheep IgG H&L (Biotin) (ab6899)
ELISA results of ab6899 against Sheep IgG. Each well was coated in duplicate with 1.0 µg of Sheep IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Streptavidin HRP conjugate and TMB substrate.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com