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AB96942

Donkey Anti-Sheep IgG H&L (DyLight® 650)

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(1 Publication)

Suitable for IHC-P, Flow Cyt, ICC/IF. Cited in 1 publication.

Key facts

Host species

Donkey

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

DyLight® 650

Excitation/Emission

Ex: 654nm, Em: 673nm

Applications

IHC-P, ICC/IF, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p>DyLight<sup>®</sup> 650 replaces DyLight<sup>®</sup> 649. The DyLight<sup>®</sup> 650 excitation is 652nm and the emission is 672nm. DyLight<sup>®</sup> 650 hence provides the same far-red fluorescence and photostability as the DyLight<sup>®</sup> 649 dye. Please refer to the vial in order to check whether the product you received is DyLight<sup>®</sup> 649 or DyLight<sup>®</sup> 650. This information however will have no impact on your experiments.</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" } } }

Product details

DyLight® 650 replaces DyLight® 649.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Lab on a chip 15:2269-77 PubMed25857666

2015

Multi-cellular 3D human primary liver cell culture elevates metabolic activity under fluidic flow.

Applications

Unspecified application

Species

Unspecified reactive species

Mandy B Esch,Jean-Matthieu Prot,Ying I Wang,Paula Miller,Jose Ricardo Llamas-Vidales,Brian A Naughton,Dawn R Applegate,Michael L Shuler
View all publications

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