AB6900

Donkey Anti-Sheep IgG H&L (HRP)

Name: Donkey Anti Sheep (IgG) secondary antibody HRP (ab6900) | Abcam
Brand: Abcam Limited
SKU: AB6900

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(28 Publications)

Suitable for Dot, IM, ICC, WB, ELISA, IHC-P, IHC-Fr. Ideal for western blot. Cited in 28 publications.

2 Images

ELISA - Donkey Anti-Sheep IgG H&L (HRP) (AB6900)

ELISA

Supplier Data

ELISA - Donkey Anti-Sheep IgG H&L (HRP) (AB6900)

ELISA results of purified ab6900 tested against purified Sheep IgG.

Each well was coated in duplicate with 1.0 μg of Sheep IgG. The starting dilution of antibody was 5 μg/ml and the X-axis represents the Log₁₀ of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC₅₀ is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.

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Western blot - Donkey Anti-Sheep IgG H&L (HRP) (AB6900)

ab6900 was used at dilution 1/20000 with the primary antibody ab30524 in WB. See the review on ab30524.

Secondary

All lanes:

Western blot - Donkey Anti-Sheep IgG H&L (HRP) (ab6900) at 1/20000 dilution

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Key facts

Host species

Donkey

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

HRP

Excitation/Emission

Applications

IM, ICC, ELISA, IHC-P, WB, IHC-Fr, Dot

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/3000 - 1/15000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/150000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/5000", "notes":"" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities

Storage buffer

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Sheep IgG

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Publications (28)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40705007

2025

Coordinated transcriptional control of the adult ventricular conduction system.

Applications

Unspecified application

Species

Unspecified reactive species

Ozanna Burnicka-Turek,Katy A Trampel,Brigitte Laforest,Michael T Broman,Xinan H Yang,Zoheb Khan,Eric Rytkin,Binjie Li,Ella Schaffer,Margaret Gadek,Kaitlyn M Shen,Igor R Efimov,Ivan P Moskowitz

Cells 13: PubMed39451251

2024

AREG Upregulation in Cancer Cells via Direct Interaction with Cancer-Associated Fibroblasts Promotes Esophageal Squamous Cell Carcinoma Progression Through EGFR-Erk/p38 MAPK Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Nakanishi,Yu-Ichiro Koma,Shoji Miyako,Rikuya Torigoe,Hiroki Yokoo,Masaki Omori,Keitaro Yamanaka,Nobuaki Ishihara,Shuichi Tsukamoto,Takayuki Kodama,Mari Nishio,Manabu Shigeoka,Hiroshi Yokozaki,Yoshihiro Kakeji

Journal of tissue engineering and regenerative medicine 2024: PubMed38571695

2024

Genetically engineered macrophages derived from iPSCs for self-regulating delivery of anti-inflammatory biologic drugs.

Applications

Unspecified application

Species

Unspecified reactive species

Molly Klimak,Farshid Guilak

The American journal of pathology 194:828-848 PubMed38320632

2024

Periostin in Cancer-Associated Fibroblasts Promotes Esophageal Squamous Cell Carcinoma Progression by Enhancing Cancer and Stromal Cell Migration.

Applications

Unspecified application

Species

Unspecified reactive species

Shoji Miyako,Yu-Ichiro Koma,Takashi Nakanishi,Shuichi Tsukamoto,Keitaro Yamanaka,Nobuaki Ishihara,Yuki Azumi,Satoshi Urakami,Masaki Shimizu,Takayuki Kodama,Mari Nishio,Manabu Shigeoka,Yoshihiro Kakeji,Hiroshi Yokozaki

The Journal of biological chemistry 298:102676 PubMed36336076

2022

The stem region of α1,6-fucosyltransferase FUT8 is required for multimer formation but not catalytic activity.

Applications

Unspecified application

Species

Unspecified reactive species

Seita Tomida,Masamichi Nagae,Yasuhiko Kizuka

Cell reports 40:111429 PubMed36170810

2022

Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Rakhee Bajaj,B Leticia Rodriguez,William K Russell,Amanda N Warner,Lixia Diao,Jing Wang,Maria G Raso,Wei Lu,Khaja Khan,Luisa S Solis,Harsh Batra,Ximing Tang,Jared F Fradette,Samrat T Kundu,Don L Gibbons

Frontiers in endocrinology 13:960279 PubMed36034417

2022

Dexamethasone-associated metabolic effects in male mice are partially caused by depletion of endogenous corticosterone.

Applications

Unspecified application

Species

Unspecified reactive species

Lisa L Koorneef,Merel van der Meulen,Sander Kooijman,Elena Sánchez-López,Jari F Scheerstra,Maaike C Voorhoeve,Ajith N Nadamuni Ramesh,Patrick C N Rensen,Martin Giera,Jan Kroon,Onno C Meijer

Hepatology communications 6:2689-2701 PubMed35833455

2022

Hepatic patatin-like phospholipase domain-containing 3 levels are increased in I148M risk allele carriers and correlate with NAFLD in humans.

Applications

Unspecified application

Species

Unspecified reactive species

Elke Ericson,Linnéa Bergenholm,Anne-Christine Andréasson,Carly I Dix,Jane Knöchel,Sara F Hansson,Richard Lee,Jennifer Schumi,Madeleine Antonsson,Ola Fjellström,Patrik Nasr,Mathias Liljeblad,Björn Carlsson,Stergios Kechagias,Daniel Lindén,Mattias Ekstedt

Experimental and therapeutic medicine 23:277 PubMed35317443

2022

Monomeric C-reactive protein level is associated with osteoarthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Yulin Liang,Ke Xu,Wenguang Liu,Xiaoling Liu,Ping Yuan,Peng Xu,Haiyun Li

The Biochemical journal 478:3869-3889 PubMed34668531

2021

AMPK activation by SC4 inhibits noradrenaline-induced lipolysis and insulin-stimulated lipogenesis in white adipose tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng-Ju Chuang,Manuel Johanns,Sébastien Pyr Dit Ruys,Gregory R Steinberg,Bruce E Kemp,Benoît Viollet,Mark H Rider

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Donkey Anti-Sheep IgG H&L (HRP)

ELISA - Donkey Anti-Sheep IgG H&L (HRP) (AB6900)

Western blot - Donkey Anti-Sheep IgG H&L (HRP) (AB6900)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Complementary Products

Secondary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (28)

Product promise