Suitable for Dot, IM, ICC/IF, WB, ELISA, IHC-P, IHC-Fr. Ideal for western blot. Cited in 24 publications.
Images
Publications
Filter by
- The Journal of biological chemistry 298:1026762022The stem region of α1,6-fucosyltransferase FUT8 is required for multimer formation but not catalytic activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeita Tomida,Masamichi Nagae,Yasuhiko KizukaPubMed 36336076
- Cell reports 40:1114292022Impad1 and Syt11 work in an epistatic pathway that regulates EMT-mediated vesicular trafficking to drive lung cancer invasion and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRakhee Bajaj,B Leticia Rodriguez,William K Russell,Amanda N Warner,Lixia Diao,Jing Wang,Maria G Raso,Wei Lu,Khaja Khan,Luisa S Solis,Harsh Batra,Ximing Tang,Jared F Fradette,Samrat T Kundu,Don L GibbonsPubMed 36170810
- Frontiers in endocrinology 13:9602792022Dexamethasone-associated metabolic effects in male mice are partially caused by depletion of endogenous corticosterone.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLisa L Koorneef,Merel van der Meulen,Sander Kooijman,Elena Sánchez-López,Jari F Scheerstra,Maaike C Voorhoeve,Ajith N Nadamuni Ramesh,Patrick C N Rensen,Martin Giera,Jan Kroon,Onno C MeijerPubMed 36034417
- Hepatology communications 6:2689-27012022Hepatic patatin-like phospholipase domain-containing 3 levels are increased in I148M risk allele carriers and correlate with NAFLD in humans.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElke Ericson,Linnéa Bergenholm,Anne-Christine Andréasson,Carly I Dix,Jane Knöchel,Sara F Hansson,Richard Lee,Jennifer Schumi,Madeleine Antonsson,Ola Fjellström,Patrik Nasr,Mathias Liljeblad,Björn Carlsson,Stergios Kechagias,Daniel Lindén,Mattias EkstedtPubMed 35833455
- Experimental and therapeutic medicine 23:2772022Monomeric C-reactive protein level is associated with osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYulin Liang,Ke Xu,Wenguang Liu,Xiaoling Liu,Ping Yuan,Peng Xu,Haiyun LiPubMed 35317443
- The Biochemical journal 478:3869-38892021AMPK activation by SC4 inhibits noradrenaline-induced lipolysis and insulin-stimulated lipogenesis in white adipose tissue.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSheng-Ju Chuang,Manuel Johanns,Sébastien Pyr Dit Ruys,Gregory R Steinberg,Bruce E Kemp,Benoît Viollet,Mark H RiderPubMed 34668531
- Cancers 13:2021Metallothionein 2A Expression in Cancer-Associated Fibroblasts and Cancer Cells Promotes Esophageal Squamous Cell Carcinoma Progression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasaki Shimizu,Yu-Ichiro Koma,Hiroki Sakamoto,Shuichi Tsukamoto,Yu Kitamura,Satoshi Urakami,Kohei Tanigawa,Takayuki Kodama,Nobuhide Higashino,Mari Nishio,Manabu Shigeoka,Yoshihiro Kakeji,Hiroshi YokozakiPubMed 34572779
- Journal of cellular and molecular medicine 25:9634-96462021Prolyl oligopeptidase inhibition reduces alpha-synuclein aggregation in a cellular model of multiple system atrophy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHengjing Cui,Tommi Kilpeläinen,Lydia Zouzoula,Samuli Auno,Kalevi Trontti,Sampo Kurvonen,Susanna Norrbacka,Iiris Hovatta,Poul Henning Jensen,Timo T MyöhänenPubMed 34486218
- Biomedicine & pharmacotherapy = Biomedecine & phar 131:1107882020Prolyl oligopeptidase inhibition by KYP-2407 increases alpha-synuclein fibril degradation in neuron-like cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinar Rostami et. al.PubMed 33152946
- Nature 585:453-4582020Epigenetic gene silencing by heterochromatin primes fungal resistance.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSito Torres-Garcia et. al.PubMed 32908306
Key facts
- Host species
- Donkey
- Target species
- Sheep
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- Dot, IM, ICC/IF, WB, ELISA, IHC-P, IHC-Fr
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Dot | Reactivity Expected | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/1000 - 1/5000 | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/3000 - 1/15000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/150000 | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/1000 - 1/5000 | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info 1/1000 - 1/5000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for Dot, IM, ICC/IF, WB, ELISA, IHC-P, IHC-Fr. Ideal for western blot. Cited in 24 publications.
Key facts
- Description
- Donkey Anti-Sheep IgG H&L (HRP)
- Host species
- Donkey
- Target species
- Sheep
- Target isotype
- IgG
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- Dot, IM, ICC/IF, WB, ELISA, IHC-P, IHC-Fr
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
ELISA - Donkey Anti-Sheep IgG H&L (HRP) (ab6900)
ELISA results of purified ab6900 tested against purified Sheep IgG.Each well was coated in duplicate with 1.0 μg of Sheep IgG. The starting dilution of antibody was 5 μg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin as blocking buffer and TMB substrate.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com