AB97125

Donkey Anti-Sheep IgG H&L (HRP)

Name: Donkey Anti Sheep (IgG) secondary antibody HRP (ab97125) | Abcam
Brand: Abcam Limited
SKU: AB97125
Rating: 3 (1 reviews)

(1 Review)

(10 Publications)

Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 10 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

Key facts

Host species

Donkey

Target species

Sheep

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Pre-adsorbed

Conjugation

HRP

Excitation/Emission

Applications

IHC-P, ELISA, ICC, WB

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/5000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"(primary)" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/5000 - 1/50000", "notes":"Colorimetric: 1/500 - 1/30000, Chemiluminescent: 1/10000 - 1/50000" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Sheep IgG

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Cell reports 37:109888 PubMed34706234

2021

Mitophagy antagonism by ZIKV reveals Ajuba as a regulator of PINK1 signaling, PKR-dependent inflammation, and viral invasion of tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Sanket S Ponia,Shelly J Robertson,Kristin L McNally,Gayatri Subramanian,Gail L Sturdevant,Matthew Lewis,Forrest Jessop,Catherine Kendall,Dylan Gallegos,Arielle Hay,Cindi Schwartz,Rebecca Rosenke,Greg Saturday,Catherine M Bosio,Craig Martens,Sonja M Best

Frontiers in pharmacology 12:701456 PubMed34163368

2021

Engineering the Nitroreductase NfsA to Create a Flexible Enzyme-Prodrug Activation System.

Applications

Unspecified application

Species

Unspecified reactive species

Abigail V Sharrock,Sarah P McManaway,Michelle H Rich,Jeff S Mumm,Ian F Hermans,Moana Tercel,Frederik B Pruijn,David F Ackerley

Cell stem cell 28:846-862.e8 PubMed33784497

2021

3D iPSC modeling of the retinal pigment epithelium-choriocapillaris complex identifies factors involved in the pathology of macular degeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Kannan V Manian,Chad A Galloway,Sonal Dalvi,Anthony A Emanuel,Jared A Mereness,Whitney Black,Lauren Winschel,Celia Soto,Yiming Li,Yuanhui Song,William DeMaria,Akhilesh Kumar,Igor Slukvin,Michael P Schwartz,William L Murphy,Bela Anand-Apte,Mina Chung,Danielle S W Benoit,Ruchira Singh

eLife 8: PubMed31241463

2019

Fetal and trophoblast PI3K p110α have distinct roles in regulating resource supply to the growing fetus in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jorge López-Tello,Vicente Pérez-García,Jaspreet Khaira,Laura C Kusinski,Wendy N Cooper,Adam Andreani,Imogen Grant,Edurne Fernández de Liger,Brian Yh Lam,Myriam Hemberger,Ionel Sandovici,Miguel Constancia,Amanda N Sferruzzi-Perri

eLife 8: PubMed31012847

2019

Endocytic recycling and vesicular transport systems mediate transcytosis of across cell monolayer.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Li,Kai-Xuan Li,Wei-Lin Hu,David M Ojcius,Jia-Qi Fang,Shi-Jun Li,Xu'ai Lin,Jie Yan

Experimental and therapeutic medicine 15:247-253 PubMed29375687

2018

Morphological and molecular comparisons between tibialis anterior muscle and levator veli palatini muscle: A preliminary study on their augmentation potential.

Applications

Unspecified application

Species

Unspecified reactive species

Xu Cheng,Lei Song,Min Lan,Bing Shi,Jingtao Li

The Journal of clinical investigation 127:681-694 PubMed28112682

2017

Nasal neuron PET imaging quantifies neuron generation and degeneration.

Applications

Species

Unspecified reactive species

Genevieve C Van de Bittner,Misha M Riley,Luxiang Cao,Janina Ehses,Scott P Herrick,Emily L Ricq,Hsiao-Ying Wey,Michael J O'Neill,Zeshan Ahmed,Tracey K Murray,Jaclyn E Smith,Changning Wang,Frederick A Schroeder,Mark W Albers,Jacob M Hooker

PloS one 11:e0160912 PubMed27513961

2016

Metabolic Response to Heat Stress in Late-Pregnant and Early Lactation Dairy Cows: Implications to Liver-Muscle Crosstalk.

Applications

Species

Unspecified reactive species

Franziska Koch,Ole Lamp,Mehdi Eslamizad,Joachim Weitzel,Björn Kuhla

Endocrinology 157:346-57 PubMed26465199

2015

Cardiomyocyte and Vascular Smooth Muscle-Independent 11β-Hydroxysteroid Dehydrogenase 1 Amplifies Infarct Expansion, Hypertrophy, and the Development of Heart Failure After Myocardial Infarction in Male Mice.

Applications

Species

Unspecified reactive species

Christopher I White,Maurits A Jansen,Kieran McGregor,Katie J Mylonas,Rachel V Richardson,Adrian Thomson,Carmel M Moran,Jonathan R Seckl,Brian R Walker,Karen E Chapman,Gillian A Gray

The Journal of experimental biology 216:3215-21 PubMed23685967

2013

Activation of systemic, but not local, renin-angiotensin system is associated with upregulation of TNF-α during prolonged fasting in northern elephant seal pups.

Applications

Unspecified application

Species

Unspecified reactive species

Miwa Suzuki,José Pablo Vázquez-Medina,Jose A Viscarra,José G Soñanez-Organis,Daniel E Crocker,Rudy M Ortiz

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Donkey Anti-Sheep IgG H&L (HRP)

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (10)

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