JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB175680

Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 405)

Be the first to review this product! Submit a review

|

(2 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 2 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

2 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 405) (AB175680)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 405) (AB175680)

ICC/IF image of ab80952 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab80952, 10μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175680 Alexa Fluor® 405 goat anti-Armenian Hamster IgG (H+L) used at 2μg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 405) (AB175680)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 405) (AB175680)
  • Unconjugated

    Goat Anti-Armenian hamster IgG H&L

  • Biotin

    Goat Anti-Armenian hamster IgG H&L (Biotin)

  • 615 Texas Red®

    Goat Anti-Armenian hamster IgG H&L (Texas Red ®)

  • 572 TRITC

    Goat Anti-Armenian hamster IgG H&L (TRITC)

  • 519 Alexa Fluor® 488

    Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488)

  • 665 Alexa Fluor® 647

    Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647)

  • 775 Alexa Fluor® 750

    Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 750)

  • 519 FITC

    Goat Anti-Armenian hamster IgG H&L (FITC)

  • 603 Alexa Fluor® 568

    Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 568)

Key facts

Host species

Goat

Target species

Armenian hamster

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

ICC/IF, Flow Cyt, IHC-P, IHC-Fr, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p>We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor<sup>®</sup> 405 dye (see difference below)<br>Ex max: Alexa Fluor<sup>®</sup> 405 = 402nm / DAPI = 359nm<br>Em max: Alexa Fluor<sup>®</sup> 405 = 421nm / DAPI = 461nm</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }

Product details

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 5:624-635 PubMed32405577

2020

High-affinity mutant Interleukin-13 targeted CAR T cells enhance delivery of clickable biodegradable fluorescent nanoparticles to glioblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Gloria B Kim,Virginia Aragon-Sanabria,Lauren Randolph,Hali Jiang,Joshua A Reynolds,Becky S Webb,Achuthamangalam Madhankumar,Xiaojun Lian,James R Connor,Jian Yang,Cheng Dong

Nature medicine 23:938-944 PubMed28714991

2017

Resolution of inflammation by interleukin-9-producing type 2 innate lymphoid cells.

Applications

IF

Species

Unspecified reactive species

Simon Rauber,Markus Luber,Stefanie Weber,Lisa Maul,Alina Soare,Thomas Wohlfahrt,Neng-Yu Lin,Katharina Dietel,Aline Bozec,Martin Herrmann,Mark H Kaplan,Benno Weigmann,Mario M Zaiss,Ursula Fearon,Douglas J Veale,Juan D Cañete,Oliver Distler,Felice Rivellese,Costantino Pitzalis,Markus F Neurath,Andrew N J McKenzie,Stefan Wirtz,Georg Schett,Jörg H W Distler,Andreas Ramming
View all publications

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com