Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488)

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) (AB173003)

ICC/IF image of ab243248 stained Neuro2a cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab243248, 1.6μg/ml) overnight at +4°C. The secondary antibody (green) was ab173003 Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

• Flow Cyt

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Flow Cytometry - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) (AB173003)

Flow cytometric analysis of Mouse spleen cells treated with 1ug/ml of immobilized anti-CD3 and 10ug/ml anti-CD28 for 3 days (Green and Black) / Untreated control (Magenta and Grey) cells labelling CD30 with ab324907 at 1/100 dilution (1ug) (Magenta and Green) compared with a Armenian Hamster monoclonal IgG (Black and Grey) isotype control.

Goat anti-Armenian hamster IgG (Alexa Fluor® 488, ab173003) at 1/5000 dilution was used as the secondary antibody.

Gated on viable CD8 positive cells.

• Flow Cyt

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Flow Cytometry - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) (AB173003)

Flow cytometric analysis of Mouse spleen cells treated with 2.5ug/ml Concanavalin A for 72 hours (Green and Black) / Untreated control (Magenta and Grey) cells labelling CD30 with ab324907 at 1/100 dilution (1ug) (Magenta and Green) compared with a Armenian Hamster monoclonal IgG (Black and Grey) isotype control.

Goat anti-Armenian hamster IgG (Alexa Fluor® 488, ab173003) at 1/5000 dilution was used as the secondary antibody.

Gated on viable CD8 positive cells.

• Flow Cyt

Supplier Data

Flow Cytometry - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) (AB173003)

Flow cytometric analysis of NIH/3T3 (mouse embryonic fibroblast, Left) / YAC-1 (mouse Moloney leukemia virus induced lymphoma lymphoblast, Right) cells labelling CD30 with ab324907 at 1/100 dilution (1ug) (Red) compared with a Armenian Hamster monoclonal IgG (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti-Armenian hamster IgG (Alexa Fluor® 488, ab173003) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Negative control : NIH/3T3.

• ICC

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Immunocytochemistry - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) (AB173003)

ICC/IF image of ab80952 stained MCF-7 cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab80952, 1μg/ml) overnight at +4°C. The secondary antibody (green) was ab173003 Alexa Fluor 488 Goat polyclonal Secondary Antibody to Armenian Hamster IgG - (H+L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

• Alexa Fluor®

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Alexa Fluor® - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488) (AB173003)