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AB173004

Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647)

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(19 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 19 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

3 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647) (AB173004)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647) (AB173004)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (red) was ab173004 Alexa Fluor® 647 goat anti-armenian hamster IgG (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Immunocytochemistry/ Immunofluorescence - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647) (AB173004)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647) (AB173004)

ICC/IF image of ab173004 stained MCF7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab80952, 10μg/ml) overnight at +4°C. The secondary antibody (red) was ab173004 Alexa Fluor® 647 goat anti-armenian hamster IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Alexa Fluor® - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647) (AB173004)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647) (AB173004)

  • Unconjugated

    Goat Anti-Armenian hamster IgG H&L

  • Biotin

    Goat Anti-Armenian hamster IgG H&L (Biotin)

  • 615 Texas Red®

    Goat Anti-Armenian hamster IgG H&L (Texas Red ®)

  • 572 TRITC

    Goat Anti-Armenian hamster IgG H&L (TRITC)

  • 519 Alexa Fluor® 488

    Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488)

  • 421 Alexa Fluor® 405

    Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 405)

  • 775 Alexa Fluor® 750

    Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 750)

  • 519 FITC

    Goat Anti-Armenian hamster IgG H&L (FITC)

  • 603 Alexa Fluor® 568

    Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 568)

Key facts

Host species

Goat

Target species

Armenian hamster

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 647

Excitation/Emission

Ex: 650nm, Em: 665nm

Applications

Flow Cyt, ELISA, IHC-Fr, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Armenian hamster IgG
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Publications (19)

Recent publications for all applications. Explore the full list and refine your search

Biomolecules 13: PubMed37759735

2023

Extracellular Acetylated Histone 3.3 Induces Inflammation and Lung Tissue Damage.

Applications

Unspecified application

Species

Unspecified reactive species

Mario C Rico,Oscar Perez-Leal,Mary F Barbe,Mamta Amin,Dennis J Colussi,Magda L Florez,Victor Olusajo,Dennise S Rios,Carlos A Barrero

Journal of virology 97:e0155722 PubMed37133449

2023

Colocalization of Chikungunya Virus with Its Receptor MXRA8 during Cell Attachment, Internalization, and Membrane Fusion.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Feng,Ellen M Bouma,Gaowei Hu,Yunkai Zhu,Yin Yu,Jolanda M Smit,Michael S Diamond,Rong Zhang

Nature communications 13:5925 PubMed36207325

2022

Nanoparticulate cell-free DNA scavenger for treating inflammatory bone loss in periodontitis.

Applications

Unspecified application

Species

Unspecified reactive species

Hanyao Huang,Weiyi Pan,Yifan Wang,Hye Sung Kim,Dan Shao,Baoding Huang,Tzu-Chieh Ho,Yeh-Hsing Lao,Chai Hoon Quek,Jiayu Shi,Qianming Chen,Bing Shi,Shengmin Zhang,Lei Zhao,Kam W Leong

mBio 13:e0105522 PubMed35699372

2022

Membrane-Tethered Mucin 1 Is Stimulated by Interferon and Virus Infection in Multiple Cell Types and Inhibits Influenza A Virus Infection in Human Airway Epithelium.

Applications

Unspecified application

Species

Unspecified reactive species

Ethan Iverson,Kira Griswold,Daniel Song,Talita B Gagliardi,Kajal Hamidzadeh,Mehmet Kesimer,Sanju Sinha,Melissa Perry,Gregg A Duncan,Margaret A Scull

Nature neuroscience 25:26-38 PubMed34916658

2021

TREM2 interacts with TDP-43 and mediates microglial neuroprotection against TDP-43-related neurodegeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Manling Xie,Yong U Liu,Shunyi Zhao,Lingxin Zhang,Dale B Bosco,Yuan-Ping Pang,Jun Zhong,Udit Sheth,Yuka A Martens,Na Zhao,Chia-Chen Liu,Yongxian Zhuang,Liewei Wang,Dennis W Dickson,Mark P Mattson,Guojun Bu,Long-Jun Wu

Investigative ophthalmology & visual science 62:17 PubMed34673901

2021

Retinal VEGF-A Overexpression Is Not Sufficient to Induce Lymphangiogenesis Regardless of VEGF-C Upregulation and Lyve1+ Macrophage Infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Iori Wada,Shintaro Nakao,Muneo Yamaguchi,Yoshihiro Kaizu,Mitsuru Arima,Shinichiro Sawa,Koh-Hei Sonoda

Cell reports 36:109610 PubMed34433040

2021

Dual role for CXCL12 signaling in semilunar valve development.

Applications

Unspecified application

Species

Unspecified reactive species

Liam A Ridge,Dania Kewbank,Dagmar Schütz,Ralf Stumm,Peter J Scambler,Sarah Ivins

Nature communications 12:3624 PubMed34131132

2021

Lasp1 regulates adherens junction dynamics and fibroblast transformation in destructive arthritis.

Applications

Unspecified application

Species

Unspecified reactive species

Denise Beckmann,Anja Römer-Hillmann,Annika Krause,Uwe Hansen,Corinna Wehmeyer,Johanna Intemann,David J J de Gorter,Berno Dankbar,Jan Hillen,Marianne Heitzmann,Isabell Begemann,Milos Galic,Toni Weinhage,Dirk Foell,Rizi Ai,Joachim Kremerskothen,Hans P Kiener,Sylvia Müller,Thomas Kamradt,Christopher Schröder,Elsa Leitão,Bernhard Horsthemke,Philip Rosenstiel,Karl Nordström,Gilles Gasparoni,Nina Gasparoni,Jörn Walter,Na Li,Xinyi Yang,Ho-Ryun Chung,Hermann Pavenstädt,Nico Lindemann,Hans J Schnittler,Wei Wang,Gary S Firestein,Thomas Pap,Adelheid Korb-Pap

Frontiers in pharmacology 12:695561 PubMed34135763

2021

Cajanolactone A, a Stilbenoid From (L.) Millsp, Prevents High-Fat Diet-Induced Obesity via Suppressing Energy Intake.

Applications

Unspecified application

Species

Unspecified reactive species

Zhuohui Luo,Jiawen Huang,Zhiping Li,Zhiwen Liu,Linchun Fu,Yingjie Hu,Xiaoling Shen

Scientific reports 10:9850 PubMed32555231

2020

Distribution of nerve fibers and nerve-immune cell association in mouse spleen revealed by immunofluorescent staining.

Applications

Unspecified application

Species

Unspecified reactive species

Dailun Hu,Huda A M Al-Shalan,Zhongli Shi,Penghao Wang,Yongkang Wu,Philip K Nicholls,Wayne K Greene,Bin Ma
View all publications
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