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AB175760

Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 750)

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Suitable for IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

2 Images
Western blot - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 750) (AB175760)
  • WB

Lab

Western blot - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 750) (AB175760)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk/0.1% TBST blocking buffer before being incubated with ab7291 overnight at 4°C. Antibody binding was detected using ab175760 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-alpha Tubulin antibody [DM1A] - Loading Control (<a href='/en-us/products/primary-antibodies/alpha-tubulin-antibody-dm1a-loading-control-ab7291'>ab7291</a>) at 1 µg/mL

All lanes:

HeLa Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 750) (ab175760) at 1/10000 dilution

false

Alexa Fluor® - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 750) (AB175760)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 750) (AB175760)
  • Unconjugated

    Goat Anti-Armenian hamster IgG H&L

  • Biotin

    Goat Anti-Armenian hamster IgG H&L (Biotin)

  • 615 Texas Red®

    Goat Anti-Armenian hamster IgG H&L (Texas Red ®)

  • 572 TRITC

    Goat Anti-Armenian hamster IgG H&L (TRITC)

  • 519 Alexa Fluor® 488

    Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 488)

  • 665 Alexa Fluor® 647

    Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 647)

  • 421 Alexa Fluor® 405

    Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 405)

  • 519 FITC

    Goat Anti-Armenian hamster IgG H&L (FITC)

  • 603 Alexa Fluor® 568

    Goat Anti-Armenian hamster IgG H&L (Alexa Fluor® 568)

Key facts

Host species

Goat

Target species

Armenian hamster

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Applications

IHC-Fr, IHC-P, Flow Cyt, ELISA, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p>This applicaiton is no longer batch tested</p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Product promise

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