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AB175674

Goat Anti-Chicken IgY H&L (Alexa Fluor® 405)

5

(1 Review)

|

(34 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Cited in 34 publications.
2 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) (AB175674)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) (AB175674)

ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes, blocked with Image-iT® FX Signal Enhancer at room temperature for 30 min and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 5μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175674 Alexa Fluor® 405 goat anti-chicken IgG (H+L) used at 1μg/ml for 1h. DRAQ5 (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) (AB175674)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) (AB175674)

Key facts

Host species

Goat

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

ICC/IF, IHC-Fr, IHC-P, ELISA, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p>We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor<sup>®</sup> 405 dye (see difference below)<br>Ex max: Alexa Fluor<sup>®</sup> 405 = 402nm / DAPI = 359nm<br>Em max: Alexa Fluor<sup>®</sup> 405 = 421nm / DAPI = 461nm</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }

Product details

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (34)

Recent publications for all applications. Explore the full list and refine your search

Addiction biology 30:e70080 PubMed40977005

2025

Chronic Intermittent Ethanol and Withdrawal Suppress Evoked and Spontaneous GABA Release Onto Distinct Populations of Basolateral Amygdala Principal Neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Michaela E Price,Hailey X Egido-Betancourt,Sarah E Sizer,Brian P Parrish,Nancy J Alexander,Kimberly F Raab-Graham,Brian A McCool

Nature 646:1243-1253 PubMed40931078

2025

Functional synapses between neurons and small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Vignesh Sakthivelu,Anna Schmitt,Franka Odenthal,Kristiano Ndoci,Marian Touet,Ali H Shaib,Abdulla Chihab,Gulzar A Wani,Pascal Nieper,Griffin G Hartmann,Isabel Pintelon,Ilmars Kisis,Maike Boecker,Naja M Eckert,Manoela Iannicelli Caiaffa,Olta Ibruli,Julia Weber,Roman Maresch,Christina M Bebber,Ali Chitsaz,Anna Lütz,Mira Kim Alves Carpinteiro,Kaylee M Morris,Camilla A Franchino,Jonas Benz,Laura Pérez-Revuelta,Jorge A Soriano-Campos,Maxim A Huetzen,Jonas Goergens,Milica Jevtic,Hannah M Jahn-Kelleter,Hans Zempel,Aleksandra Placzek,Alexandru A Hennrich,Karl-Klaus Conzelmann,Hannah L Tumbrink,Pascal Hunold,Joerg Isensee,Lisa Werr,Felix Gaedke,Astrid Schauss,Marielle Minère,Marie Müller,Henning Fenselau,Yin Liu,Alena Heimsoeth,Gülce S Gülcüler Balta,Henning Walczak,Christian Frezza,Ron D Jachimowicz,Julie George,Marcel Schmiel,Johannes Brägelmann,Tim Hucho,Silvia von Karstedt,Martin Peifer,Alessandro Annibaldi,Robert Hänsel-Hertsch,Thorsten Persigehl,Holger Grüll,Martin L Sos,Guido Reifenberger,Matthias Fischer,Dirk Adriaensen,Reinhard Büttner,Julien Sage,Inge Brouns,Roland Rad,Roman K Thomas,Max Anstötz,Silvio O Rizzoli,Matteo Bergami,Elisa Motori,Hans Christian Reinhardt,Filippo Beleggia

Science advances 11:eadw6375 PubMed40929279

2025

An early surge of norepinephrine along brainstem pathways drives sensory-evoked awakening.

Applications

Unspecified application

Species

Unspecified reactive species

Noa Matosevich,Noa Regev,Uddi Kimchy,Noam Zelinger,Sina Kabaha,Noam Gabay,Amit Marmelshtein,Yuval Nir

Glia 73:2057-2076 PubMed40631759

2025

SUR1-TRPM4 Regulates Aquaporin-4 Subcellular Localization by Astrocytic Endfeet Calcium Signals Following Status Epilepticus.

Applications

Unspecified application

Species

Unspecified reactive species

Tingting Yang,Zhenzhou Lin,Mingjia Yu,Yongchuan Li,Jiancong Chen,Yuanchi Liu,Kaibin Huang,Suyue Pan

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70272 PubMed39777689

2025

The C3/C3aR pathway exacerbates acetaminophen-induced mouse liver injury via upregulating podoplanin on the macrophage.

Applications

Unspecified application

Species

Unspecified reactive species

Zhanli Xie,Jiang Jiang,Fei Yang,Jingjing Han,Zhenni Ma,Tao Wen,Xia Bai

Developmental cell 59:2143-2157.e9 PubMed38843836

2024

Neuronal activity-driven O-GlcNAcylation promotes mitochondrial plasticity.

Applications

Unspecified application

Species

Unspecified reactive species

Seungyoon B Yu,Haoming Wang,Richard G Sanchez,Natasha M Carlson,Khanh Nguyen,Andrew Zhang,Zachary D Papich,Ahmed A Abushawish,Zachary Whiddon,Weronika Matysik,Jie Zhang,Thomas C Whisenant,Majid Ghassemian,John N Koberstein,Melissa L Stewart,Samuel A Myers,Gulcin Pekkurnaz

iScience 26:108469 PubMed38213788

2024

β-adrenergic receptor regulates embryonic epithelial extensibility through actomyosin inhibition.

Applications

Unspecified application

Species

Unspecified reactive species

Yohei Mizoguchi,Kaoru Nakashima,Ayato Sato,Asako Shindo

Nature communications 14:5804 PubMed37726325

2023

Prosaposin maintains lipid homeostasis in dopamine neurons and counteracts experimental parkinsonism in rodents.

Applications

Unspecified application

Species

Unspecified reactive species

Yachao He,Ibrahim Kaya,Reza Shariatgorji,Johan Lundkvist,Lars U Wahlberg,Anna Nilsson,Dejan Mamula,Jan Kehr,Justyna Zareba-Paslawska,Henrik Biverstål,Karima Chergui,Xiaoqun Zhang,Per E Andren,Per Svenningsson

Journal of neurophysiology 130:799-823 PubMed37609680

2023

Locomotor-related propriospinal V3 neurons produce primary afferent depolarization and modulate sensory transmission to motoneurons.

Applications

Unspecified application

Species

Unspecified reactive species

Shihao Lin,Krishnapriya Hari,Sophie Black,Aysan Khatmi,Karim Fouad,Monica A Gorassini,Yaqing Li,Ana M Lucas-Osma,Keith K Fenrich,David J Bennett

Scientific reports 13:13586 PubMed37605005

2023

Amyloid-β slows cilia movement along the ventricle, impairs fluid flow, and exacerbates its neurotoxicity in explant culture.

Applications

Unspecified application

Species

Unspecified reactive species

Ryota Makibatake,Sora Oda,Yoshiki Yagi,Hitoshi Tatsumi
View all publications

Product promise

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