Goat Anti-Chicken IgY H&L (Alexa Fluor® 405)
5
(1 Review)
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(34 Publications)
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) (AB175674)
ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes, blocked with Image-iT® FX Signal Enhancer at room temperature for 30 min and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 5μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175674 Alexa Fluor® 405 goat anti-chicken IgG (H+L) used at 1μg/ml for 1h. DRAQ5 (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
- Alexa Fluor®
Unknown
Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) (AB175674)
Reactivity data
Product details
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Shipped at conditions
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Target data
Publications (34)
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Addiction biology 30:e70080 PubMed40977005
2025
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Nature 646:1243-1253 PubMed40931078
2025
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Science advances 11:eadw6375 PubMed40929279
2025
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Glia 73:2057-2076 PubMed40631759
2025
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FASEB journal : official publication of the Federation of American Societies for Experimental Biology 39:e70272 PubMed39777689
2025
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Developmental cell 59:2143-2157.e9 PubMed38843836
2024
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iScience 26:108469 PubMed38213788
2024
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Nature communications 14:5804 PubMed37726325
2023
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Journal of neurophysiology 130:799-823 PubMed37609680
2023
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Scientific reports 13:13586 PubMed37605005
2023
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com