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AB175675

Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) preadsorbed

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(37 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 37 publications.
2 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) preadsorbed (AB175675)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) preadsorbed (AB175675)

ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab89984, 5μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175675, Alexa Fluor® 405 Goat polyclonal Secondary Antibody to Chicken IgY - H&L pre-adsorbed, used at 1μg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) preadsorbed (AB175675)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) preadsorbed (AB175675)

Key facts

Host species

Goat

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 405

Excitation/Emission

Ex: 401nm, Em: 421nm

Applications

ELISA, IHC-Fr, Flow Cyt, IHC-P, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgG and with light chains common to other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p>We recommend the use of a dedicated 405 filter for optimal results not the DAPI filter. The DAPI filter may not excite until the maximum emission peaks of Alexa Fluor<sup>®</sup> 405 dye (see difference below)<br>Ex max: Alexa Fluor<sup>®</sup> 405 = 402nm / DAPI = 359nm<br>Em max: Alexa Fluor<sup>®</sup> 405 = 421nm / DAPI = 461nm</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" } } }

Product details

We recommend storage time at 4°C should be minimal, since this may affect the signal strength.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. The antibody to chicken IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (37)

Recent publications for all applications. Explore the full list and refine your search

Nature neuroscience 28:2201-2216 PubMed41044342

2025

Muscle-derived miR-126 regulates TDP-43 axonal local synthesis and NMJ integrity in ALS models.

Applications

Unspecified application

Species

Unspecified reactive species

Ariel Ionescu,Lior Ankol,Anand Ganapathy Subramaniam,Topaz Altman,Iddo Magen,Yahel Cohen,Yehuda Danino,Tal Gradus-Pery,Yoav Niv,Ori Bar Avi,Danielle Geller,Amjd Ibraheem,Ruilei Cheng,Noam Steinberg,Leenor Alfahel,Pauline Duc,Zeynep Ergul-Ulger,Doruk Arslan,Ersin Tan,Florence Rage,Nilo Riva,Angello Quattrini,Can Ebru Bekircan-Kurt,Adrian Israelson,Amir Dori,Eran Hornstein,Eran Perlson

Scientific reports 15:25996 PubMed40676062

2025

Prenatal exposure to bisphenol A disrupts RNA splicing in the prefrontal cortex and promotes behaviors related to autism in offspring.

Applications

Unspecified application

Species

Unspecified reactive species

Pawinee Panjabud,Songphon Kanlayaprasit,Surangrat Thongkorn,Kwanjira Songsritaya,Pattanachat Lertpeerapan,Kasidit Kasitipradit,Thanawin Jantheang,Suthathip Sarobol,Thanit Saeliw,Valerie W Hu,Takeshi Imai,Tewarit Sarachana

Nature communications 15:7909 PubMed39256359

2024

Variants in LRRC7 lead to intellectual disability, autism, aggression and abnormal eating behaviors.

Applications

Unspecified application

Species

Unspecified reactive species

Jana Willim,Daniel Woike,Daniel Greene,Sarada Das,Kevin Pfeifer,Weimin Yuan,Anika Lindsey,Omar Itani,Amber L Böhme,Debora Tibbe,Hans-Hinrich Hönck,Fatemeh Hassani Nia,Michael Zech,Theresa Brunet,Laurence Faivre,Arthur Sorlin,Antonio Vitobello,Thomas Smol,Cindy Colson,Kristin Baranano,Krista Schatz,Allan Bayat,Kelly Schoch,Rebecca Spillmann,Erica E Davis,Erin Conboy,Francesco Vetrini,Konrad Platzer,Sonja Neuser,Janina Gburek-Augustat,Alexandra Noel Grace,Bailey Mitchell,Alexander Stegmann,Margje Sinnema,Naomi Meeks,Carol Saunders,Maxime Cadieux-Dion,Juliane Hoyer,Julien Van-Gils,Jean-Madeleine de Sainte-Agathe,Michelle L Thompson,E Martina Bebin,Monika Weisz-Hubshman,Anne-Claude Tabet,Alain Verloes,Jonathan Levy,Xenia Latypova,Sönke Harder,Gary A Silverman,Stephen C Pak,Tim Schedl,Kathleen Freson,Andrew Mumford,Ernest Turro,Christian Schlein,Vandana Shashi,Hans-Jürgen Kreienkamp

Nature cardiovascular research 2:1221-1245 PubMed38500966

2024

Disrupted propionate metabolism evokes transcriptional changes in the heart by increasing histone acetylation and propionylation.

Applications

Unspecified application

Species

Unspecified reactive species

Kyung Chan Park,Nicholas T Crump,Niamh Louwman,Steve Krywawych,Yuen Jian Cheong,Iolanda Vendrell,Eleanor K Gill,Mala Gunadasa-Rohling,Kerrie L Ford,David Hauton,Marjorie Fournier,Elisabete Pires,Lydia Watson,Gerald Roseman,James Holder,Andreas Koschinski,Ricardo Carnicer,M Kate Curtis,Manuela Zaccolo,Alzbeta Hulikova,Roman Fischer,Holger B Kramer,James S O McCullagh,Sophie Trefely,Thomas A Milne,Pawel Swietach

The European journal of neuroscience 59:2102-2117 PubMed38279611

2024

Schizophrenia risk proteins ZNF804A and NT5C2 interact in cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Afra Aabdien,Laura Sichlinger,Zoe Borgel,Madeleine R Jones,Iain A Waston,Nicholas J F Gatford,Pooja Raval,Lloyd Tanangonan,Timothy R Powell,Rodrigo R R Duarte,Deepak P Srivastava

Molecular neurobiology 61:693-706 PubMed37656313

2023

The Shank/ProSAP N-Terminal (SPN) Domain of Shank3 Regulates Targeting to Postsynaptic Sites and Postsynaptic Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Daniel Woike,Debora Tibbe,Fatemeh Hassani Nia,Victoria Martens,Emily Wang,Igor Barsukov,Hans-Jürgen Kreienkamp

Biology of sex differences 14:8 PubMed36803626

2023

Investigation of autism-related transcription factors underlying sex differences in the effects of bisphenol A on transcriptome profiles and synaptogenesis in the offspring hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Surangrat Thongkorn,Songphon Kanlayaprasit,Kasidit Kasitipradit,Pattanachat Lertpeerapan,Pawinee Panjabud,Valerie W Hu,Depicha Jindatip,Tewarit Sarachana

The Journal of neuroscience : the official journal of the Society for Neuroscience 43:1475-1491 PubMed36732068

2023

Synaptotagmin 9 Modulates Spontaneous Neurotransmitter Release in Striatal Neurons by Regulating Substance P Secretion.

Applications

Unspecified application

Species

Unspecified reactive species

Michael J Seibert,Chantell S Evans,Kevin S Stanley,Zhenyong Wu,Edwin R Chapman

eLife 12: PubMed36729040

2023

Synaptic vesicle proteins are selectively delivered to axons in mammalian neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Emma T Watson,Michaela M Pauers,Michael J Seibert,Jason D Vevea,Edwin R Chapman

microPublication biology 2023: PubMed36660421

2023

Epitope tag-specific differences in the detection of COSA-1 marked crossover sites in spermatocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Cori K Cahoon,Celja J Uebel,Anne M Villeneuve,Diana E Libuda
View all publications

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