Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) preadsorbed
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(37 Publications)
- ICC/IF
Lab
Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) preadsorbed (AB175675)
ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab89984, 5μg/ml) overnight at +4°C. The secondary antibody (blue) was ab175675, Alexa Fluor® 405 Goat polyclonal Secondary Antibody to Chicken IgY - H&L pre-adsorbed, used at 1μg/ml for 1h. DRAQ5™ (ab108410) was used to stain the cell nuclei (red) at a concentration of 1.25μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
- Alexa Fluor®
Unknown
Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 405) preadsorbed (AB175675)
Reactivity data
Product details
We recommend storage time at 4°C should be minimal, since this may affect the signal strength.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Publications (37)
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Nature neuroscience 28:2201-2216 PubMed41044342
2025
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Scientific reports 15:25996 PubMed40676062
2025
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Nature communications 15:7909 PubMed39256359
2024
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Nature cardiovascular research 2:1221-1245 PubMed38500966
2024
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The European journal of neuroscience 59:2102-2117 PubMed38279611
2024
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Molecular neurobiology 61:693-706 PubMed37656313
2023
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Biology of sex differences 14:8 PubMed36803626
2023
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The Journal of neuroscience : the official journal of the Society for Neuroscience 43:1475-1491 PubMed36732068
2023
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eLife 12: PubMed36729040
2023
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microPublication biology 2023: PubMed36660421
2023
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com