Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)

• IHC-Fr

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Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (AB150169)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse testis tissue labeling DDX4 with ab307373 at 1/100 dilution (10.5 µg/ml) followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining on mouse testis. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307373 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control : Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

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Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (AB150169)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling MAP2 with ab318993 at 1/2000 (0.486 ug/ml) dilution followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).

Panel A : merged staining of anti-MAP2 (ab318993, green), anti-NeuN (ab177487, red) and anti-GFAP (ab313596, grey) on rat hippocampus.
Panel B : anti-MAP2 stained on rat hippocampus.
Panel C : anti-NeuN stained in neuron of rat hippocampus.
Panel D : anti-GFAP stained in astrocytes of rat hippocampus.
The nuclear counterstain was DAPI (Blue). The section was incubated with ab318993, ab177487 and ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (AB150169)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh frozen) tissue labeling MAP2 with ab318993 at 1/2000 (0.486 ug/ml) dilution followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).

Negative control : confocal image showing no staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab318993 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (AB150169)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat testis tissue labeling DDX4 with ab307373 at 1/100 dilution (10.5 µg/ml) followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL) (Green). Confocal image showing positive staining on rat testis. The nuclear counterstain was DAPI (Blue). The section was incubated with ab307373 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). Secondary antibody control : Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL). Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (AB150169)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh frozen) tissue labeling MAP2 with ab318993 at 1/2000 (0.486 ug/ml) dilution followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).

Negative control : confocal image showing no staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with ab318993 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (AB150169)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling MAP2 with ab318993 at 1/2000 (0.486 ug/ml) dilution followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).

Panel A : merged staining of anti-MAP2 (ab318993, green), anti-NeuN (ab177487, red) and anti-GFAP (ab313596, grey) on mouse hippocampus.
Panel B : anti-MAP2 stained on mouse hippocampus.
Panel C : anti-NeuN stained in neuron of mouse hippocampus.
Panel D : anti-GFAP stained in astrocytes of mouse hippocampus.
The nuclear counterstain was DAPI (Blue). The section was incubated with ab318993, ab177487 and ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

Secondary antibody control : Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (AB150169)

ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab89984, 5μg/ml) overnight at +4°C. The secondary antibody (green) was ab150169 Alexa Fluor® 488 goat anti-chicken IgY (H+L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

• Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (AB150169)