Goat anti-chicken IgY (Alexa Fluor® 488) is a secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 200 publications
Images
Publications
Filter by
- Nature communications 14:84202023Mutant GGGGCC RNA prevents YY1 from binding to Fuzzy promoter which stimulates Wnt/β-catenin pathway in C9ALS/FTD.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhefan Stephen Chen,Mingxi Ou,Stephanie Taylor,Ruxandra Dafinca,Shaohong Isaac Peng,Kevin Talbot,Ho Yin Edwin ChanPubMed 38110419
- Frontiers in immunology 14:12254822023LncRNA xist regulates sepsis associated neuroinflammation in the periventricular white matter of CLP rats by miR-122-5p/PKCη Axis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHuifang Wang,Yichen Li,Shuqi Jiang,Nan Liu,Qiuping Zhou,Qian Li,Zhuo Chen,Yiyan Lin,Chunbo Chen,Yiyu DengPubMed 38115999
- Cells 12:2023Wnt/β-Catenin-Signaling Modulates Megakaryopoiesis at the Megakaryocyte-Erythrocyte Progenitor Stage in the Hematopoietic System.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBurak H Yalcin,Jadranka Macas,Eliza Wiercinska,Patrick N Harter,Malak Fawaz,Tessa Schmachtel,Ilaria Ghiro,Ewa Bieniek,Djuro Kosanovic,Sonja Thom,Marcus Fruttiger,Makoto M Taketo,Ralph T Schermuly,Michael A Rieger,Karl H Plate,Halvard Bonig,Stefan LiebnerPubMed 38067194
- Nature methods 21:680-6912023Improved green and red GRAB sensors for monitoring dopaminergic activity in vivo.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYizhou Zhuo,Bin Luo,Xinyang Yi,Hui Dong,Xiaolei Miao,Jinxia Wan,John T Williams,Malcolm G Campbell,Ruyi Cai,Tongrui Qian,Fengling Li,Sophia J Weber,Lei Wang,Bozhi Li,Yu Wei,Guochuan Li,Huan Wang,Yu Zheng,Yulin Zhao,Marina E Wolf,Yingjie Zhu,Mitsuko Watabe-Uchida,Yulong LiPubMed 38036855
- Acta neuropathologica communications 11:1822023Neuronal dysfunction caused by FUSR521G promotes ALS-associated phenotypes that are attenuated by NF-κB inhibition.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMari Carmen Pelaez,Antoine Desmeules,Pauline A Gelon,Bastien Glasson,Laetitia Marcadet,Alicia Rodgers,Daniel Phaneuf,Silvia Pozzi,Paul A Dutchak,Jean-Pierre Julien,Chantelle F SephtonPubMed 37974279
- Nature communications 14:73462023CHEX-seq detects single-cell genomic single-stranded DNA with catalytical potential.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYoutao Lu,Jaehee Lee,Jifen Li,Srinivasa Rao Allu,Jinhui Wang,HyunBum Kim,Kevin L Bullaughey,Stephen A Fisher,C Erik Nordgren,Jean G Rosario,Stewart A Anderson,Alexandra V Ulyanova,Steven Brem,H Isaac Chen,John A Wolf,M Sean Grady,Sergei A Vinogradov,Junhyong Kim,James EberwinePubMed 37963886
- Science advances 9:eadg38442023A cellular resolution atlas of Broca's area.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrene Costantini,Leah Morgan,Jiarui Yang,Yael Balbastre,Divya Varadarajan,Luca Pesce,Marina Scardigli,Giacomo Mazzamuto,Vladislav Gavryusev,Filippo Maria Castelli,Matteo Roffilli,Ludovico Silvestri,Jessie Laffey,Sophia Raia,Merina Varghese,Bridget Wicinski,Shuaibin Chang,Ichun Anderson Chen,Hui Wang,Devani Cordero,Matthew Vera,Jackson Nolan,Kimberly Nestor,Jocelyn Mora,Juan Eugenio Iglesias,Erendira Garcia Pallares,Kathryn Evancic,Jean C Augustinack,Morgan Fogarty,Adrian V Dalca,Matthew P Frosch,Caroline Magnain,Robert Frost,Andre van der Kouwe,Shih-Chi Chen,David A Boas,Francesco Saverio Pavone,Bruce Fischl,Patrick R HofPubMed 37824623
- International journal of molecular sciences 24:2023Sex Differences in Brain Region-Specific Activation of c-Fos following Kappa Opioid Receptor Stimulation or Acute Stress in Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianhan Ma,Susan Wonnacott,Sarah J Bailey,Christopher P BaileyPubMed 37894779
- Molecular therapy. Methods & clinical development 31:1011292023Non-canonical amino acid incorporation into AAV5 capsid enhances lung transduction in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHao Chang,Ailing Du,Jun Jiang,Lingzhi Ren,Nan Liu,Xuntao Zhou,Jialing Liang,Guangping Gao,Dan WangPubMed 37886602
- PloS one 18:e02910292023Functional characterization of a single nucleotide polymorphism associated with Alzheimer's disease in a hiPSC-based neuron model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLindsay R Stolzenburg,Sahar Esmaeeli,Ameya S Kulkarni,Erin Murphy,Taekyung Kwon,Christina Preiss,Lamiaa Bahnassawy,Joshua D Stender,Justine D Manos,Peter Reinhardt,Fedik Rahimov,Jeffrey F Waring,Cyril Y RamathalPubMed 37751459
Key facts
- Host species
- Goat
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Goat anti-chicken IgY (Alexa Fluor® 488) is a secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 200 publications
Key facts
- Description
- Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)
- Host species
- Goat
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 488
- Excitation/Emission
- Ex: 495nm, Em: 519nm
- Applications
- ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse testis tissue labeling DDX4 with Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) - BSA and Azide free ab307373 at 1/100 dilution (10.5 µg/ml) followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL) (Green).
Confocal image showing positive staining on mouse testis.
The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) - BSA and Azide free ab307373 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab89984, 5μg/ml) overnight at +4°C. The secondary antibody (green) was ab150169 Alexa Fluor® 488 goat anti-chicken IgY (H+L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/2000 (0.486 ug/ml) dilution followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).
Panel A: merged staining of anti-MAP2 (Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993, green), anti-NeuN (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487, red) and anti-GFAP (Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596, grey) on mouse hippocampus.
Panel B: anti-MAP2 stained on mouse hippocampus.
Panel C: anti-NeuN stained in neuron of mouse hippocampus.
Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993, Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 and Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody control: Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat testis tissue labeling DDX4 with Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) - BSA and Azide free ab307373 at 1/100 dilution (10.5 µg/ml) followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL) (Green).
Confocal image showing positive staining on rat testis.
The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) - BSA and Azide free ab307373 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh frozen) tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/2000 (0.486 ug/ml) dilution followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).
Negative control: confocal image showing no staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh frozen) tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/2000 (0.486 ug/ml) dilution followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).
Negative control: confocal image showing no staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.
Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) (ab150169)
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/2000 (0.486 ug/ml) dilution followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).
Panel A: merged staining of anti-MAP2 (Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993, green), anti-NeuN (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487, red) and anti-GFAP (Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596, grey) on rat hippocampus.
Panel B: anti-MAP2 stained on rat hippocampus.
Panel C: anti-NeuN stained in neuron of rat hippocampus.
Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993, Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 and Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Secondary antibody control: Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com