Goat anti-chicken IgY (Alexa Fluor® 488) is a secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 200 publications
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Goat anti-chicken IgY (Alexa Fluor® 488) is a secondary antibody with a maximum absorption wavelength of 495nm and a maximum emission wavelength of 519nm. Ideal for fluorescent cell and tissue imaging. Suitable for ICC/IF, IHC, flow cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 200 publications
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
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Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse testis tissue labeling DDX4 with Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) - BSA and Azide free ab307373 at 1/100 dilution (10.5 µg/ml) followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL) (Green).
Confocal image showing positive staining on mouse testis.
The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) - BSA and Azide free ab307373 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal donkey serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the primary antibody (ab89984, 5μg/ml) overnight at +4°C. The secondary antibody (green) was ab150169 Alexa Fluor® 488 goat anti-chicken IgY (H+L) used at 1μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse hippocampus (fresh frozen) tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/2000 (0.486 ug/ml) dilution followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).
Panel A: merged staining of anti-MAP2 (Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993, green), anti-NeuN (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487, red) and anti-GFAP (Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596, grey) on mouse hippocampus.
Panel B: anti-MAP2 stained on mouse hippocampus.
Panel C: anti-NeuN stained in neuron of mouse hippocampus.
Panel D: anti-GFAP stained in astrocytes of mouse hippocampus.
The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993, Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 and Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen rat testis tissue labeling DDX4 with Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) - BSA and Azide free ab307373 at 1/100 dilution (10.5 µg/ml) followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL) (Green).
Confocal image showing positive staining on rat testis.
The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) - BSA and Azide free ab307373 for 60 mins at room temperature. The section was then mounted using Fluoromount®. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 dilution (2 µg/mL).
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat skeletal muscle (fresh frozen) tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/2000 (0.486 ug/ml) dilution followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).
Negative control: confocal image showing no staining on rat skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse skeletal muscle (fresh frozen) tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/2000 (0.486 ug/ml) dilution followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).
Negative control: confocal image showing no staining on mouse skeletal muscle. The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.
Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat hippocampus (fresh frozen) tissue labeling MAP2 with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993 at 1/2000 (0.486 ug/ml) dilution followed by ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488) at 1/1000 2 ug/mL dilution (Green).
Panel A: merged staining of anti-MAP2 (Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993, green), anti-NeuN (Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487, red) and anti-GFAP (Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596, grey) on rat hippocampus.
Panel B: anti-MAP2 stained on rat hippocampus.
Panel C: anti-NeuN stained in neuron of rat hippocampus.
Panel D: anti-GFAP stained in astrocytes of rat hippocampus.
The nuclear counterstain was DAPI (Blue). The section was incubated with Anti-MAP2 antibody [EPR19691] - Chicken IgY (Chimeric) ab318993, Anti-NeuN antibody [EPR12763] - Neuronal Marker ab177487 and Alexa Fluor® 647 Anti-GFAP antibody [EPR1034Y] - Mouse IgG1 Chimeric ab313596 for 60 mins at room temperature. The section was then mounted using Fluoromount®.The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Secondary antibody control: Secondary antibody is ab150169 Goat Anti-Chicken IgY H&L (Alexa Fluor® 488)at 1/1000 2 ug/mL dilution.
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