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AB150174

Goat Anti-Chicken IgY H&L (Alexa Fluor® 555) preadsorbed

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(9 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Preadsorbed to minimise non-specific binding and high background staining. Cited in 9 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

3 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 555) preadsorbed (AB150174)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 555) preadsorbed (AB150174)

ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 10μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab150174, goat polyclonal secondary antibody to chicken IgY - H&L (Alexa Fluor® 555), pre-adsorbed, used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 555) preadsorbed (AB150174)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 555) preadsorbed (AB150174)

HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (yellow) was ab150174, goat polyclonal secondary antibody to chicken IgY - H&L (Alexa Fluor® 555), pre-adsorbed, used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 555) preadsorbed (AB150174)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 555) preadsorbed (AB150174)

Key facts

Host species

Goat

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 555

Excitation/Emission

Ex: 555nm, Em: 565nm

Applications

ICC/IF, Flow Cyt, IHC-Fr, ELISA, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgG and with light chains common to other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to chicken IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Chicken IgY
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Brain, behavior, and immunity 119:251-260 PubMed38552924

2024

Instability of excitatory synapses in experimental autoimmune encephalomyelitis and the outcome for excitatory circuit inputs to individual cortical neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Rebecca L Gillani,Eseza N Kironde,Sara Whiteman,Theodore J Zwang,Brian J Bacskai

Frontiers in neuroscience 18:1361590 PubMed38406586

2024

Enhancing regeneration and repair of long-distance peripheral nerve defect injuries with continuous microcurrent electrical nerve stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Junjie Kong,Cheng Teng,Fenglan Liu,Xuzhaoyu Wang,Yi Zhou,Ying Zong,Zixin Wan,Jun Qin,Bin Yu,Daguo Mi,Yaxian Wang

Nature aging 2:837-850 PubMed37118504

2022

Complement C1q-dependent excitatory and inhibitory synapse elimination by astrocytes and microglia in Alzheimer's disease mouse models.

Applications

Unspecified application

Species

Unspecified reactive species

Borislav Dejanovic,Tiffany Wu,Ming-Chi Tsai,David Graykowski,Vineela D Gandham,Christopher M Rose,Corey E Bakalarski,Hai Ngu,Yuanyuan Wang,Shristi Pandey,Mitchell G Rezzonico,Brad A Friedman,Rose Edmonds,Ann De Mazière,Raphael Rakosi-Schmidt,Tarjinder Singh,Judith Klumperman,Oded Foreman,Michael C Chang,Luke Xie,Morgan Sheng,Jesse E Hanson

Cell reports 31:107778 PubMed32553154

2020

Combinatorial Developmental Controls on Striatonigral Circuits.

Applications

Unspecified application

Species

Unspecified reactive species

Ayano Matsushima,Ann M Graybiel

eLife 8: PubMed31439126

2019

Two-step regulation of ensures tight coupling of cell fate with morphogenesis in the trachea.

Applications

Unspecified application

Species

Unspecified reactive species

Takefumi Kondo,Shigeo Hayashi

Journal of neurophysiology 121:1591-1608 PubMed30625007

2019

5-HT receptors inhibit the monosynaptic stretch reflex by modulating C-fiber activity.

Applications

Unspecified application

Species

Unspecified reactive species

Ana M Lucas-Osma,Yaqing Li,Katie Murray,Shihao Lin,Sophie Black,Marilee J Stephens,Andrew H Ahn,C J Heckman,Keith K Fenrich,Karim Fouad,David J Bennett

The Journal of physiology 596:4581-4595 PubMed30019338

2018

Sympathoexcitation by hypothalamic paraventricular nucleus neurons projecting to the rostral ventrolateral medulla.

Applications

Unspecified application

Species

Unspecified reactive species

Satoshi Koba,Eri Hanai,Nao Kumada,Naoya Kataoka,Kazuhiro Nakamura,Tatsuo Watanabe

Journal of materials chemistry. B 6:2204-2216 PubMed30294445

2018

The in vivo fates of plant viral nanoparticles camouflaged using self-proteins: overcoming immune recognition.

Applications

Unspecified application

Species

Unspecified reactive species

N M Gulati,A S Pitek,A E Czapar,P L Stewart,N F Steinmetz

Nature medicine 23:733-741 PubMed28459438

2017

Pericytes impair capillary blood flow and motor function after chronic spinal cord injury.

Applications

Unspecified application

Species

Unspecified reactive species

Yaqing Li,Ana M Lucas-Osma,Sophie Black,Mischa V Bandet,Marilee J Stephens,Romana Vavrek,Leo Sanelli,Keith K Fenrich,Antonio F Di Narzo,Stella Dracheva,Ian R Winship,Karim Fouad,David J Bennett
View all publications
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