Goat Anti-Chicken IgY H&L (Alexa Fluor® 568)
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(36 Publications)
- ICC/IF
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 568) (AB175477)
ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 5μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175477 Alexa Fluor® 568 goat anti-chicken IgY used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
- Alexa Fluor®
Unknown
Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 568) (AB175477)
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Publications (36)
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Cell reports methods 5:101047 PubMed40359937
2025
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The European journal of neuroscience 60:7228-7248 PubMed39622242
2024
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Cell reports 43:114873 PubMed39423130
2024
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Neurospine 21:878-889 PubMed39363468
2024
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iScience 27:109878 PubMed38799556
2024
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The Journal of cell biology 223: PubMed38713825
2024
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ACS pharmacology & translational science 7:667-679 PubMed38481685
2024
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PloS one 18:e0291029 PubMed37751459
2023
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Journal of the Endocrine Society 7:bvad113 PubMed37693686
2023
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The Journal of clinical investigation 133: PubMed37104042
2023
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