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AB175477

Goat Anti-Chicken IgY H&L (Alexa Fluor® 568)

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(36 Publications)

Suitable for ELISA, IHC-Fr, IHC-P, Flow Cyt, ICC/IF. Ideal for fluorescent cell and tissue imaging. Cited in 36 publications.
2 Images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 568) (AB175477)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 568) (AB175477)

ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 5μg/ml) overnight at +4°C. The secondary antibody (yellow) was ab175477 Alexa Fluor® 568 goat anti-chicken IgY used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 568) (AB175477)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 568) (AB175477)

Key facts

Host species

Goat

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 568

Excitation/Emission

Ex: 578nm, Em: 603nm

Applications

ICC/IF, Flow Cyt, ELISA, IHC-P, IHC-Fr

applications

Clonality

Polyclonal

Isotype

IgG

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" } } }

Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (36)

Recent publications for all applications. Explore the full list and refine your search

Cell reports methods 5:101047 PubMed40359937

2025

Harmonizing TUNEL with multiplexed iterative immunofluorescence enriches spatial contextualization of cell death.

Applications

Unspecified application

Species

Unspecified reactive species

Marc S Sherman,Thomas McMahon-Skates,Lindsey S Gaston,Sonya W Katzen,Joseph A Majzoub,Wolfram Goessling

The European journal of neuroscience 60:7228-7248 PubMed39622242

2024

Evolving alterations of structural organization and functional connectivity in feedforward neural networks after induced P301L tau mutation.

Applications

Unspecified application

Species

Unspecified reactive species

Janelle S Weir,Katrine Sjaastad Hanssen,Nicolai Winter-Hjelm,Axel Sandvig,Ioanna Sandvig

Cell reports 43:114873 PubMed39423130

2024

Heterogeneity in oligodendrocyte precursor cell proliferation is dynamic and driven by passive bioelectrical properties.

Applications

Unspecified application

Species

Unspecified reactive species

Helena Pivoňková,Sergey Sitnikov,Yasmine Kamen,An Vanhaesebrouck,Moritz Matthey,Sonia Olivia Spitzer,Yan Ting Ng,Chenyue Tao,Omar de Faria,Balazs Viktor Varga,Ragnhildur Thóra Káradóttir

Neurospine 21:878-889 PubMed39363468

2024

Modulation of the LIMK Pathway by Myricetin: A Protective Strategy Against Neurological Impairments in Spinal Cord Injury.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Roy,Santimoy Sen,Rudradip Das,Amit Shard,Hemant Kumar

iScience 27:109878 PubMed38799556

2024

Optimization of AAV vectors for transactivator-regulated enhanced gene expression within targeted neuronal populations.

Applications

Unspecified application

Species

Unspecified reactive species

Leo Kojima,Kaoru Seiriki,Hiroki Rokujo,Takanobu Nakazawa,Atsushi Kasai,Hitoshi Hashimoto

The Journal of cell biology 223: PubMed38713825

2024

Actomyosin-II protects axons from degeneration induced by mild mechanical stress.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaorong Pan,Yiqing Hu,Gaowei Lei,Yaxuan Wei,Jie Li,Tongshu Luan,Yunfan Zhang,Yuanyuan Chu,Yu Feng,Wenrong Zhan,Chunxia Zhao,Frédéric A Meunier,Yifan Liu,Yi Li,Tong Wang

ACS pharmacology & translational science 7:667-679 PubMed38481685

2024

Investigating LIM (Lin-11, Isl-1, and Mec-3) Kinases and Their Correlation with Pathological Events and Microtubule Dynamics in an Experimental Model of Spinal Cord Injury in Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Abhishek Roy,Zarna Pathak,Hemant Kumar

PloS one 18:e0291029 PubMed37751459

2023

Functional characterization of a single nucleotide polymorphism associated with Alzheimer's disease in a hiPSC-based neuron model.

Applications

Unspecified application

Species

Unspecified reactive species

Lindsay R Stolzenburg,Sahar Esmaeeli,Ameya S Kulkarni,Erin Murphy,Taekyung Kwon,Christina Preiss,Lamiaa Bahnassawy,Joshua D Stender,Justine D Manos,Peter Reinhardt,Fedik Rahimov,Jeffrey F Waring,Cyril Y Ramathal

Journal of the Endocrine Society 7:bvad113 PubMed37693686

2023

Validation of a new Custom Polyclonal Progesterone Receptor Antibody for Immunohistochemistry in the Female Mouse Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Amy Ruddenklau,Kelly Glendining,Melanie Prescott,Rebecca E Campbell

The Journal of clinical investigation 133: PubMed37104042

2023

Postoperative risk of IDH-mutant glioma-associated seizures and their potential management with IDH-mutant inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Michael R Drumm,Wenxia Wang,Thomas K Sears,Kirsten Bell-Burdett,Rodrigo Javier,Kristen Y Cotton,Brynna Webb,Kayla Byrne,Dusten Unruh,Vineeth Thirunavu,Jordain Walshon,Alicia Steffens,Kathleen McCortney,Rimas V Lukas,Joanna J Phillips,Esraa Mohamed,John D Finan,Lucas Santana-Santos,Amy B Heimberger,Colin K Franz,Jonathan Kurz,Jessica W Templer,Geoffrey T Swanson,Craig Horbinski
View all publications

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