Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed

12 product images

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

  ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 10μg/ml) overnight at +4°C. The secondary antibody (orange) was ab150176 Alexa Fluor® 594 goat anti-chicken IgY (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

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  This image is courtesy of an anonymous customer review

  Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

  Gel Running Conditions: Reduced Denaturing (15% PAGE)
  

  Detection method: Fluorescent Secondary Antibodies

  All lanes: Western blot - Anti-GFP antibody (Anti-GFP antibody ab13970) at 1/2000 dilution

  Lane 1: 3 µg of GFP plasmid overexpressed in mouse cardiomyocytes whole cell lysate at 25 µg with BSA / for 1 hour at room temperature

  Lane 2: 2 µg of GFP plasmid overexpressed in mouse cardiomyocytes whole cell lysate at 25 µg with BSA / for 1 hour at room temperature

  Lane 3: 1 µg of GFP plasmid overexpressed in mouse cardiomyocytes whole cell lysate at 25 µg with BSA / for 1 hour at room temperature

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176) at 1/5000 dilution

  Performed under reducing conditions.

  Predicted band size: 27 kDa

  Observed band size: 25 kDa

  Exposure time: 30s

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

  HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (orange) was ab150176 Alexa Fluor® 594 goat anti-chicken IgY (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

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  Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)
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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

  Immunofluorescence staining of GFAP using Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054 in primary rat hippocampal mixed glia, (prepared from P2 rat hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. SDPHP4m), DIV4. The cells were fixed with 100% MeOH (5 min), permeabilized with 0.1% Triton-X-100 (in PBS) for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054 at 0.1 μg/ml and Anti-GFAP antibody - Astrocyte Marker ab4674, Anti-GFAP antibody, at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150176, Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
  Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown. The antibody Anti-GFAP antibody [RM1003] - Astrocyte Marker ab278054 gave comparable results using 4% formaldehyde fixation (10 min).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

  Immunofluorescence staining of SOX9 using Alexa Fluor® 488 Anti-SOX9 antibody [EPR14335] ab196450 in primary hippocampal mouse neurons/glia (obtained from Transnetyx Tissue by BrainBits LLC cat.no. C57EHP) DIV14. The cells were fixed with 4% formaldehyde (10 min) permeabilized with 0.1% TritonX-100 (in PBS) for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Alexa Fluor® 488 Anti-SOX9 antibody [EPR14335] ab196450 at 1 μg/ml (shown in green), Anti-GFAP antibody - Astrocyte Marker ab4674 (anti-GFAP) at 1/1000 dilution and Anti-NeuN antibody [1B7] - Neuronal Marker ab104224 (anti-NeuN) at 1/1000 dilution. Cells were then incubated with ab150176 Goat Anti-Chicken IgY H&L (Alexa Fluor^® 594) preadsorbed (shown in red) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 647) (shown in purple) all secondary antibodies at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).
  As expected most GFAP positive cells are also SOX9 positive while NeuN positive cells are SOX9 negative.
  Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

  Immunofluorescence staining of Iba-1 using Anti-Iba1 antibody [EPR16588] ab178846 in primary rat hippocampal mixed glia (prepared from P2 rat hippocampal brain area obtained from Transnetyx Tissue by BrainBits LLC cat.no. SDPHP4m) DIV4. The cells were fixed with 4% formaldehyde (10 min) permeabilized with 0.1% Triton-X-100 (in PBS) for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-Iba1 antibody [EPR16588] ab178846 at 0.1 μg/ml and Anti-GFAP antibody - Astrocyte Marker ab4674 Anti-GFAP antibody at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150176 Goat Anti-Chicken IgY H&L (Alexa Fluor^® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown. The antibody Anti-Iba1 antibody [EPR16588] ab178846 gave comparable results using MeOH fixation (100% 5 min).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

  Immunofluorescence staining of SOX9 using Anti-SOX9 antibody [EPR14335] ab185230 in primary hippocampal mouse neurons/glia (obtained from Transnetyx Tissue by BrainBits LLC cat.no. C57EHP) DIV14. The cells were fixed with 4% formaldehyde (10 min) permeabilized with 0.1% TritonX-100 (in PBS) for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-SOX9 antibody [EPR14335] ab185230 at 5 μg/ml, Anti-GFAP antibody - Astrocyte Marker ab4674 (anti-GFAP) at 1/1000 dilution and Anti-NeuN antibody [1B7] - Neuronal Marker ab104224 (anti-NeuN) at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) ab150176 Goat Anti-Chicken IgY H&L (Alexa Fluor^® 594) preadsorbed (shown in red) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed ab150119 Goat Anti-Mouse IgG H&L (Alexa Fluor^® 647) (shown in purple) all secondary antibodies at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).
  As expected most GFAP positive cells are also SOX9 positive while NeuN positive cells are SOX9 negative.
  Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

  Immunofluorescence staining of SOX9 using Anti-SOX9 antibody [EPR14335-78] ab185966 in primary hippocampal mouse neurons/glia, (obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. C57EHP), DIV14. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% TritonX-100 (in PBS) for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-SOX9 antibody [EPR14335-78] ab185966 at 1 μg/ml, Anti-GFAP antibody - Astrocyte Marker ab4674 (anti-GFAP) at 1/1000 dilution and Anti-NeuN antibody [1B7] - Neuronal Marker ab104224 (anti-NeuN) at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green), ab150176, Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (shown in red) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) preadsorbed ab150119, Goat Anti-Mouse IgG H&L (Alexa Fluor® 647) (shown in purple), all secondary antibodies at 1/1000 dilution. Nuclear DNA was labelled with DAPI (shown in blue).
  As expected, most GFAP positive cells are also SOX9 positive, while NeuN positive cells are SOX9 negative. SOX9 positive cells, which are not GFAP positive (e.g. asterisk) are likely neural stem cells/ oligodendrocyte precursor cells present in the culture.
  Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown.

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

  Immunofluorescence staining of GFAP using Anti-GFAP antibody [EP672Y] - Astrocyte Marker ab33922 in primary rat hippocampal mixed glia, (prepared from P2 rat hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. SDPHP4m), DIV4. The cells were fixed with 100% MeOH (5 min), permeabilized with 0.1% Triton-X-100 (in PBS) for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-GFAP antibody [EP672Y] - Astrocyte Marker ab33922 at 0.2 μg/ml and Anti-GFAP antibody - Astrocyte Marker ab4674, Anti-GFAP antibody, at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150176, Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
  Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown. The antibody Anti-GFAP antibody [EP672Y] - Astrocyte Marker ab33922 gave comparable results using 4% formaldehyde fixation (10 min).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

  Immunofluorescence staining of GFAP using Anti-GFAP antibody [EPR19996] - Astrocyte Marker ab207165 in primary rat hippocampal mixed glia, (prepared from P2 rat hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. SDPHP4m), DIV4. The cells were fixed with 100% MeOH (5 min), permeabilized with 0.1% Triton-X-100 (in PBS) for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-GFAP antibody [EPR19996] - Astrocyte Marker ab207165 at 0.1 μg/ml and Anti-GFAP antibody - Astrocyte Marker ab4674, Anti-GFAP antibody, at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150176, Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
  Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown. The antibody Anti-GFAP antibody [EPR19996] - Astrocyte Marker ab207165 gave comparable results using 4% formaldehyde fixation (10 min).

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  Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (ab150176)

  Immunofluorescence staining of GFAP using Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab68428 in primary rat hippocampal mixed glia, (prepared from P2 rat hippocampal brain area, obtained from Transnetyx Tissue by BrainBits, LLC, cat.no. SDPHP4m), DIV4. The cells were fixed with 100% MeOH (5 min), permeabilized with 0.1% Triton-X-100 (in PBS) for 5 mins and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab68428 at 0.1 μg/ml and Anti-GFAP antibody - Astrocyte Marker ab4674, Anti-GFAP antibody, at 1/1000 dilution. Cells were then incubated with Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150176, Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).
  Images were acquired with the Perkin Elmer Operetta HCA and a maximum intensity projection of confocal sections is shown. The antibody Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker ab68428 gave comparable results using 4% formaldehyde fixation (10 min).