Goat Anti-Chicken IgY H&L (Alexa Fluor® 647)

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (AB150171)

ICC/IF image of ab89984 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 5μg/ml) overnight at +4°C. The secondary antibody (red) was ab150171 Alexa Fluor^® 647 goat anti-chicken IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.

The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (AB150171)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Shootin-1 with ab322654 at 1/100 (5.06 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in mouse primary neural/glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 um followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab5392 Anti-MAP2 chicken polyclonal antibody was used to counterstain tubulin at 1/ 500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab322654 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution. -ve control 2 : ab5392 Anti-MAP2 chicken polyclonal antibody at 1/ 500 4ug/ml dilution, followed by ab150171 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) antibody at 1/1000 dilution.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (AB150171)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Shootin-1 with ab322654 at 1/100 (5.06 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in rat primary neural/glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 um followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab5392 Anti-MAP2 chicken polyclonal antibody was used to counterstain tubulin at 1/500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

-ve control 1 : ab322654 at 1/100 dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution. -ve control 2 : ab5392 Anti-MAP2 chicken polyclonal antibody at 1/ 500 4ug/ml dilution, followed by ab150171 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) antibody at 1/1000 dilution.

• Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (AB150171)