Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 50 publications
Images
Publications
Filter by
- iScience 27:1092882024The RNA binding proteins LARP4A and LARP4B promote sarcoma and carcinoma growth and metastasis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJennifer C Coleman,Luke Tattersall,Val Yianni,Laura Knight,Hongqiang Yu,Sadie R Hallett,Philip Johnson,Ana J Caetano,Charlie Cosstick,Anne J Ridley,Alison Gartland,Maria R Conte,Agamemnon E GrigoriadisPubMed 38532886
- eNeuro 10:2023A Novel Flp Reporter Mouse Shows That TRPA1 Expression Is Largely Limited to Sensory Neuron Subsets.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMayur J Patil,Seol-Hee Kim,Parmvir K Bahia,Sanjay S Nair,Teresa S Darcey,Jailene Fiallo,Xiao Xia Zhu,Robert D Frisina,Stephen H Hadley,Thomas E Taylor-ClarkPubMed 37989590
- Science advances 9:eadg38442023A cellular resolution atlas of Broca's area.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIrene Costantini,Leah Morgan,Jiarui Yang,Yael Balbastre,Divya Varadarajan,Luca Pesce,Marina Scardigli,Giacomo Mazzamuto,Vladislav Gavryusev,Filippo Maria Castelli,Matteo Roffilli,Ludovico Silvestri,Jessie Laffey,Sophia Raia,Merina Varghese,Bridget Wicinski,Shuaibin Chang,Ichun Anderson Chen,Hui Wang,Devani Cordero,Matthew Vera,Jackson Nolan,Kimberly Nestor,Jocelyn Mora,Juan Eugenio Iglesias,Erendira Garcia Pallares,Kathryn Evancic,Jean C Augustinack,Morgan Fogarty,Adrian V Dalca,Matthew P Frosch,Caroline Magnain,Robert Frost,Andre van der Kouwe,Shih-Chi Chen,David A Boas,Francesco Saverio Pavone,Bruce Fischl,Patrick R HofPubMed 37824623
- STAR protocols 4:1025022023Attachment culture of cortical organoids at the microwell air-liquid interface.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJiyuan Tang,Honghui Zheng,Yilin Feng,Junhong Zeng,Shaohua MaPubMed 37715950
- Development (Cambridge, England) 150:2023The RNF220 domain nuclear factor Teyrha-Meyrha (Tey) regulates the migration and differentiation of specific visceral and somatic muscles in Drosophila.Applications:Unspecified applicationReactive species:Unspecified reactive speciesManfred Frasch,Afshan Ismat,Ingolf Reim,Jasmin RauferPubMed 37642089
- Biomaterials research 27:712023Astrocytic scar restricting glioblastoma via glutamate-MAO-B activity in glioblastoma-microglia assembloid.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYen N Diep,Hee Jung Park,Joon-Ho Kwon,Minh Tran,Hae Young Ko,Hanhee Jo,Jisu Kim,Jee-In Chung,Tai Young Kim,Dongwoo Kim,Jong Hee Chang,You Jung Kang,C Justin Lee,Mijin Yun,Hansang ChoPubMed 37468961
- The Journal of clinical investigation 133:2023Postoperative risk of IDH-mutant glioma-associated seizures and their potential management with IDH-mutant inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMichael R Drumm,Wenxia Wang,Thomas K Sears,Kirsten Bell-Burdett,Rodrigo Javier,Kristen Y Cotton,Brynna Webb,Kayla Byrne,Dusten Unruh,Vineeth Thirunavu,Jordain Walshon,Alicia Steffens,Kathleen McCortney,Rimas V Lukas,Joanna J Phillips,Esraa Mohamed,John D Finan,Lucas Santana-Santos,Amy B Heimberger,Colin K Franz,Jonathan Kurz,Jessica W Templer,Geoffrey T Swanson,Craig HorbinskiPubMed 37104042
- Scientific reports 13:76892023A CRISPR-engineered isogenic model of the 22q11.2 A-B syndromic deletion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNeha Paranjape,Yu-Hsiu T Lin,Quetzal Flores-Ramirez,Vishesh Sarin,Amanda Brooke Johnson,Julia Chu,Mercedes Paredes,Arun P WiitaPubMed 37169815
- Frontiers in immunology 14:11306972023USP10 is a potential mediator for vagus nerve stimulation to alleviate neuroinflammation in ischaemic stroke by inhibiting NF-κB signalling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChenchen Xie,Xiang Gao,Gang Liu,Hao Tang,Changqing LiPubMed 37153558
- Science advances 9:eadg66182023Primate-conserved carbonic anhydrase IV and murine-restricted LY6C1 enable blood-brain barrier crossing by engineered viral vectors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTimothy F Shay,Erin E Sullivan,Xiaozhe Ding,Xinhong Chen,Sripriya Ravindra Kumar,David Goertsen,David Brown,Anaya Crosby,Jost Vielmetter,Máté Borsos,Damien A Wolfe,Annie W Lam,Viviana GradinaruPubMed 37075114
Key facts
- Host species
- Goat
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) is a secondary antibody with a maximum absorption wavelength of 650nm and a maximum emission wavelength of 665nm. Ideal for fluorescent cell and tissue imaging. Suitable for IHC, ICC/IF, Flow Cytometry and ELISA.
- Intense fluorescence and high photostability allowing more time for image capture especially when detecting low abundance targets
- Minimal spectral overlap with other Alexa Fluor® dyes makes this ideal for use in multi-color analysis
- Cited in over 50 publications
Key facts
- Description
- Goat Anti-Chicken IgY H&L (Alexa Fluor® 647)
- Host species
- Goat
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Conjugation
- Alexa Fluor® 647
- Excitation/Emission
- Ex: 650nm, Em: 665nm
- Applications
- ELISA, IHC-Fr, IHC-P, ICC/IF, Flow Cyt
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
This antibody reacts with the whole molecule chicken IgY
Fluorochrome chart - a complete quick and easy guide to help you select the most appropriate fluorochromes for your next experiment.
When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (ab150171)
ICC/IF image of ab89984 stained HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 5μg/ml) overnight at +4°C. The secondary antibody (red) was ab150171 Alexa Fluor® 647 goat anti-chicken IgG (H+L) used at 2μg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43μM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (ab150171)
Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (ab150171)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized rat primary neural/glia cell cells labelling Shootin-1 with Anti-Shootin-1 antibody [EPR29089-46] ab322654 at 1/100 (5.06 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in rat primary neural/glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 um followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody - Neuronal Marker ab5392 Anti-MAP2 chicken polyclonal antibody was used to counterstain tubulin at 1/Anti-MAP2 antibody - Neuronal Marker ab5392 500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
-ve control 1: Anti-Shootin-1 antibody [EPR29089-46] ab322654 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.
-ve control 2: Anti-MAP2 antibody - Neuronal Marker ab5392 Anti-MAP2 chicken polyclonal antibody at 1/ 500 4ug/ml dilution, followed by ab150171 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) antibody at 1/1000 dilution.
Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (ab150171)
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neural/glia cell cells labelling Shootin-1 with Anti-Shootin-1 antibody [EPR29089-46] ab322654 at 1/100 (5.06 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in mouse primary neural/glia cell (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Confocal scanning Z step was set as 0.3 um followed by image processing with maximum Z projection. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
Anti-MAP2 antibody - Neuronal Marker ab5392 Anti-MAP2 chicken polyclonal antibody was used to counterstain tubulin at 1/ 500 4ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
-ve control 1: Anti-Shootin-1 antibody [EPR29089-46] ab322654 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/ 1000 2ug/ml dilution.
-ve control 2: Anti-MAP2 antibody - Neuronal Marker ab5392 Anti-MAP2 chicken polyclonal antibody at 1/ 500 4ug/ml dilution, followed by ab150171 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) antibody at 1/1000 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com