Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed

7 product images

• 

  This image is courtesy of a customer review submitted by Alex Zagariya.

  Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (ab150175)

  Anti-GFAP antibody ab4674 staining GFAP in rabbit eye tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with formaldehyde and blocked with BSA for 2 hours at 4°C. Samples were incubated with primary antibody (1/1000) for 12 hours at 4°C. An Alexa Fluor® 647-conjugated Goat anti-chicken IgY H&L (ab150175) (1/1000) was used as the secondary antibody.

• 

  Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (ab150175)

  ICC/IF image of ab89984 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab89984, 10µg/ml) overnight at +4°C. The secondary antibody (red) was ab150175 Alexa Fluor® 647 goat anti-chicken IgY (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (ab150175)

  HeLa cells showing negative staining by ICC/IF using only secondary antibody. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The secondary antibody (red) was ab150175 Alexa Fluor® 647 goat anti-chicken IgY (H+L) used at 2µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

• 

  Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (ab150175)
• 

  Flow Cytometry (Intracellular) - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (ab150175)

  Flow Cytometry (Intracellular) analysis of 293T transfected with GFP tagged Firefly Luciferase over expression vector labeling GFP (right) with Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643 at a 1/500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti-Chicken IgY H&L (Alexa Fluor® 647, ab150175) was used as the secondary antibody at a 1/5000 dilution.  Chicken IgY Isotype control. (left)

• 

  Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (ab150175)

  Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized HEK293T (human embryonic kidney epithelial cells) labeling GFP with Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643 at 1/50 dilution followed by ab150175 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (red). Confocal image showing positive staining in HEK-293T cells transfected with a Firefly Luciferase expression vector containing a GFP tag. The nuclear counter stain is DAPI (blue).

• 

  Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed (ab150175)

  Immunofluorescent analysis of 4% Paraformaldehyde fixed, 0.1% Triton X-100 permeabilized 293/GFP (human embryonic kidney epithelial cell) cells labeling GFP with Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643 at 1/50 dilution followed by ab150175 Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) preadsorbed secondary antibody at 1/1000 (2 μg/ml) dilution (red). Confocal image showing positive staining in 293/GFP cells. The nuclear counter stain is DAPI (blue).