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AB175779

Goat Anti-Chicken IgY H&L (Alexa Fluor® 680)

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(5 Publications)

Suitable for WB. Cited in 5 publications.
1 Images
Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 680) (AB175779)
  • WB

Lab

Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 680) (AB175779)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab83956 overnight at 4°C. Antibody binding was detected using ab175779 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (<a href='/en-us/products/primary-antibodies/gapdh-antibody-loading-control-ab83956'>ab83956</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 680) (ab175779) at 1/10000 dilution

Observed band size: 37 kDa

false

Key facts

Host species

Goat

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Alexa Fluor® 680

Excitation/Emission

Ex: 679nm, Em: 702nm

Applications

WB

applications

Clonality

Polyclonal

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" } } }

Product details

We batch test Goat Anti-Chicken IgY H&L (Alexa Fluor® 680), ab175779 in fluorescent WB. Although we don't batch test for ICC, ELISA, IHC-Fr or Flow cytometry customers have had success usingGoat Anti-Chicken IgY H&L (Alexa Fluor® 680), ab175779 in these applications.

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
The antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark

Product protocols

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40298577

2025

A toolbox for ablating excitatory and inhibitory synapses.

Applications

Unspecified application

Species

Unspecified reactive species

Aida Bareghamyan,Changfeng Deng,Sarah Daoudi,Shubhash C Yadav,Xiaocen Lu,Wei Zhang,Robert E Campbell,Richard H Kramer,David M Chenoweth,Don B Arnold

Nature metabolism 7:714-729 PubMed40200126

2025

TMEM65 regulates and is required for NCLX-dependent mitochondrial calcium efflux.

Applications

Unspecified application

Species

Unspecified reactive species

Joanne F Garbincius,Oniel Salik,Henry M Cohen,Carmen Choya-Foces,Adam S Mangold,Angelina D Makhoul,Anna E Schmidt,Dima Y Khalil,Joshua J Doolittle,Anya S Wilkinson,Emma K Murray,Michael P Lazaropoulos,Alycia N Hildebrand,Dhanendra Tomar,John W Elrod

Frontiers in neuroscience 18:1328815 PubMed38601090

2024

An MR-based brain template and atlas for optical projection tomography and light sheet fluorescence microscopy in neuroscience.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie M A Willekens,Federico Morini,Tomas Mediavilla,Emma Nilsson,Greger Orädd,Max Hahn,Nunya Chotiwan,Montse Visa,Per-Olof Berggren,Erwin Ilegems,Anna K Överby,Ulf Ahlgren,Daniel Marcellino

Cell 185:379-396.e38 PubMed35021063

2022

Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches.

Applications

Unspecified application

Species

Unspecified reactive species

Martin Guilliams,Johnny Bonnardel,Birthe Haest,Bart Vanderborght,Camille Wagner,Anneleen Remmerie,Anna Bujko,Liesbet Martens,Tinne Thoné,Robin Browaeys,Federico F De Ponti,Bavo Vanneste,Christian Zwicker,Freya R Svedberg,Tineke Vanhalewyn,Amanda Gonçalves,Saskia Lippens,Bert Devriendt,Eric Cox,Giuliano Ferrero,Valerie Wittamer,Andy Willaert,Suzanne J F Kaptein,Johan Neyts,Kai Dallmeier,Peter Geldhof,Stijn Casaert,Bart Deplancke,Peter Ten Dijke,Anne Hoorens,Aude Vanlander,Frederik Berrevoet,Yves Van Nieuwenhove,Yvan Saeys,Wouter Saelens,Hans Van Vlierberghe,Lindsey Devisscher,Charlotte L Scott

Immunity 51:638-654.e9 PubMed31561945

2019

Stellate Cells, Hepatocytes, and Endothelial Cells Imprint the Kupffer Cell Identity on Monocytes Colonizing the Liver Macrophage Niche.

Applications

Unspecified application

Species

Unspecified reactive species

Johnny Bonnardel,Wouter T'Jonck,Djoere Gaublomme,Robin Browaeys,Charlotte L Scott,Liesbet Martens,Bavo Vanneste,Sofie De Prijck,Sergei A Nedospasov,Anna Kremer,Evelien Van Hamme,Peter Borghgraef,Wendy Toussaint,Pieter De Bleser,Inge Mannaerts,Alain Beschin,Leo A van Grunsven,Bart N Lambrecht,Tom Taghon,Saskia Lippens,Dirk Elewaut,Yvan Saeys,Martin Guilliams
View all publications

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