Suitable for WB, IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.
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Publications
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- European cells & materials 41:774-7922021A murine Staphylococcus aureus fracture-related infection model characterised by fracture non-union, staphylococcal abscess communities and myeloid-derived suppressor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM I Hofstee,M Riool,F Gieling,V Stenger,C Constant,D Nehrbass,S Zeiter,R G Richards,S Aj Zaat,T F MoriartyPubMed 34151416
Key facts
- Host species
- Goat
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Rabbit, Horse, Cow, Human, Pig
- Conjugation
- Alexa Fluor® 750
- Excitation/Emission
- Ex: 749nm, Em: 775nm
- Applications
- WB, IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/10000 | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes This applicaiton is no longer batch tested |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes This applicaiton is no longer batch tested |
Application ELISA | Reactivity Reacts | Dilution info - | Notes This applicaiton is no longer batch tested |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes This applicaiton is no longer batch tested |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes This applicaiton is no longer batch tested |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for WB, IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.
Key facts
- Description
- Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) preadsorbed
- Host species
- Goat
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Mouse, Rat, Rabbit, Horse, Cow, Human, Pig
- Conjugation
- Alexa Fluor® 750
- Excitation/Emission
- Ex: 749nm, Em: 775nm
- Applications
- WB, IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgG and with light chains common to other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to chicken IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle, Stable for 12 months at -20°C, Store in the dark
Notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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2 product images
Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) preadsorbed (ab175755)
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Anti-GAPDH antibody - Loading Control ab83956 overnight at 4°C. Antibody binding was detected using ab175755 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-GAPDH antibody - Loading Control (Anti-GAPDH antibody - Loading Control ab83956) at 1 µg/mL
All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) preadsorbed (ab175755) at 1/10000 dilution
Observed band size: 37 kDa
Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) preadsorbed (ab175755)
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