Suitable for WB, IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/10000 | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes This applicaiton is no longer batch tested |
Application ICC/IF | Reactivity Reacts | Dilution info 1/200.00000 - 1/1000.00000 | Notes This applicaiton is no longer batch tested |
Application ELISA | Reactivity Reacts | Dilution info - | Notes This applicaiton is no longer batch tested |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes This applicaiton is no longer batch tested |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/2000 | Notes This applicaiton is no longer batch tested |
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Suitable for WB, IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.
By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgG and with light chains common to other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.
Preservative: 0.02% Sodium azide
Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to chicken IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
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This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with Anti-GAPDH antibody - Loading Control ab83956 overnight at 4°C. Antibody binding was detected using ab175755 at a 1:10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.
All lanes: Western blot - Anti-GAPDH antibody - Loading Control (Anti-GAPDH antibody - Loading Control ab83956) at 1 µg/mL
All lanes: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg
All lanes: Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) preadsorbed (ab175755) at 1/10000 dilution
Observed band size: 37 kDa
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