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AB175755

Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) preadsorbed

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(3 Publications)

Suitable for WB, IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cyt. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.
2 Images
Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) preadsorbed (AB175755)
  • WB

Lab

Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) preadsorbed (AB175755)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Milk before being incubated with ab83956 overnight at 4°C. Antibody binding was detected using ab175755 at a 1 : 10,000 dilution for 1hr at room temperature and then imaged using the Licor Odyssey CLx.

All lanes:

Western blot - Anti-GAPDH antibody - Loading Control (<a href='/en-us/products/primary-antibodies/gapdh-antibody-loading-control-ab83956'>ab83956</a>) at 1 µg/mL

All lanes:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) preadsorbed (ab175755) at 1/10000 dilution

Observed band size: 37 kDa

false

Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) preadsorbed (AB175755)
  • Alexa Fluor®

Unknown

Alexa Fluor® - Goat Anti-Chicken IgY H&L (Alexa Fluor® 750) preadsorbed (AB175755)

Key facts

Host species

Goat

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Alexa Fluor® 750

Excitation/Emission

Ex: 749nm, Em: 775nm

Applications

ELISA, IHC-P, WB, ICC/IF, IHC-Fr, Flow Cyt

applications

Clonality

Polyclonal

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with chicken IgG and with light chains common to other chicken immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat IgG was detected. This antibody may cross react with IgG from other species.

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Secondary Antibodies

AB150171

Goat Anti-Chicken IgY H&L (Alexa Fluor® 647)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 647) (AB150171)

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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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Secondary Antibodies

AB150176

Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 594) preadsorbed (AB150176)

  • 5
  • 3
Secondary Antibodies

AB150170

Goat Anti-Chicken IgY H&L (Alexa Fluor® 555)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Chicken IgY H&L (Alexa Fluor® 555) (AB150170)

  • 0
  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>This applicaiton is no longer batch tested</p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000", "notes":"<p>This applicaiton is no longer batch tested</p>" } } }
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Product details

Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive Antibodies. The antibody to chicken IgG was isolated by affinity chromatography using antigen coupled to agarose beads.
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS, 23% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle|Stable for 12 months at -20°C|Store in the dark
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Product protocols

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Target data

See full target information Chicken IgY
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:26814 PubMed40702232

2025

Cell-specific α and β subunit expression patterns of Na/K-ATPases in the common marmoset cochlea.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Hosoya,Masafumi Ueno,Marie N Shimanuki,Takanori Nishiyama,Naoki Oishi,Hiroyuki Ozawa

Scientific reports 14:25166 PubMed39448766

2024

A primate model animal revealed the inter-species differences and similarities in the subtype specifications of the spiral ganglion neurons.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Hosoya,Masafumi Ueno,Marie N Shimanuki,Takanori Nishiyama,Naoki Oishi,Hiroyuki Ozawa

European cells & materials 41:774-792 PubMed34151416

2021

A murine Staphylococcus aureus fracture-related infection model characterised by fracture non-union, staphylococcal abscess communities and myeloid-derived suppressor cells.

Applications

Unspecified application

Species

Unspecified reactive species

M I Hofstee,M Riool,F Gieling,V Stenger,C Constant,D Nehrbass,S Zeiter,R G Richards,S Aj Zaat,T F Moriarty
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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