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AB7119

Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed

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Suitable for WB, Dot, IHC-Fr, IHC-P, IM, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining.
1 Images
Western blot - Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed (AB7119)
  • WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed (AB7119)

Western blot analysis of ab7119 used as a secondary antibody at 1/1000 dilution for 60 min at RT.

All lanes:

Chicken IgG/IgY at 50 ng

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed (ab7119) at 1/1000 dilution

false

Key facts

Host species

Goat

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Hamster, Cow, Human,

Pre-adsorbed

Yes

Conjugation

Alkaline Phosphatase

Excitation/Emission
Applications

IM, IHC-P, ELISA, WB, IHC-Fr, Dot, ICC/IF

applications

Clonality

Polyclonal

Specificity

No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2500", "notes":"<p></p>" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-Fr": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/2000 - 1/10000", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer.
Storage buffer
pH: 8 Preservative: 0.01% Sodium azide Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

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