Suitable for WB, Dot, IHC-Fr, IHC-P, IM, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining.
Images
Key facts
- Host species
- Goat
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Hamster, Cow, Human
- Conjugation
- Alkaline Phosphatase
- Applications
- WB, Dot, IHC-Fr, IHC-P, IM, ELISA, ICC/IF
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/500 - 1/2500 | Notes - |
Application Dot | Reactivity Expected | Dilution info - | Notes - |
Application IHC-Fr | Reactivity Expected | Dilution info - | Notes - |
Application IHC-P | Reactivity Expected | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application ELISA | Reactivity Expected | Dilution info 1/2000 - 1/10000 | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/200 - 1/1000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for WB, Dot, IHC-Fr, IHC-P, IM, ELISA, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining.
Key facts
- Description
- Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed
- Host species
- Goat
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Minimal cross-reactivity
- Rat, Sheep, Rabbit, Goat, Horse, Guinea pig, Hamster, Cow, Human
- Conjugation
- Alkaline Phosphatase
- Applications
- WB, Dot, IHC-Fr, IHC-P, IM, ELISA, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- Yes
- Specificity
No reaction was observed against Bovine, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rabbit, Rat and Sheep Serum Proteins. This antibody will react with heavy chains of Chicken IgG and with light chains of most Chicken immunoglobulins.
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 8
Preservative: 0.01% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA, 0.88% Sodium chloride, 0.788% Tris HCl, 0.00952% Magnesium chloride, 0.00136% Zinc chloride- Purification technique
- Affinity purification
- Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads followed by conjugation to fluorochrome and extensive dialysis against the buffer.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Product promise
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Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed (ab7119)
Western blot analysis of ab7119 used as a secondary antibody at 1/1000 dilution for 60 min at RT.
All lanes: Chicken IgG/IgY at 50 ng
SecondaryAll lanes: Western blot - Goat Anti-Chicken IgY H&L (Alkaline Phosphatase) preadsorbed (ab7119) at 1/1000 dilution
Downloads
Product protocols
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