Suitable for Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF. Cited in 11 publications.
Images
Publications
Filter by
- Communications biology 5:9802022Inhibition of the medial amygdala disrupts escalated aggression in lactating female mice after repeated exposure to male intruders.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMaría Abellán-Álvaro,Fernando Martínez-García,Enrique Lanuza,Carmen Agustín-PavónPubMed 36114351
- Particle and fibre toxicology 19:272022Dose-response relationship of pulmonary disorders by inhalation exposure to cross-linked water-soluble acrylic acid polymers in F344 rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomoki Takeda,Shotaro Yamano,Yuko Goto,Shigeyuki Hirai,Yusuke Furukawa,Yoshinori Kikuchi,Kyohei Misumi,Masaaki Suzuki,Kenji Takanobu,Hideki Senoh,Misae Saito,Hitomi Kondo,George Daghlian,Young-Kwon Hong,Yasuhiro Yoshimatsu,Masanori Hirashima,Yoichiro Kobashi,Kenzo Okamoto,Takumi Kishimoto,Yumi UmedaPubMed 35395797
- Scientific reports 11:65512021Hericium erinaceus mycelium and its small bioactive compounds promote oligodendrocyte maturation with an increase in myelin basic protein.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui-Ting Huang,Chia-Hsin Ho,Hsin-Yu Sung,Li-Ya Lee,Wan-Ping Chen,Yu-Wen Chen,Chin-Chu Chen,Chung-Shi Yang,Shun-Fen TzengPubMed 33753806
- Behavioural brain research 405:1132062021Silencing and stimulating the medial amygdala impairs ejaculation but not sexual incentive motivation in male rats.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPatty T Huijgens,Roy Heijkoop,Eelke M S SnoerenPubMed 33639266
- Stem cell research 40:1015322019Xenogen-free isolation and culture of human adipose mesenchymal stem cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesM Doornaert et. al.PubMed 31421383
- Journal of burn care & research : official publication of the American Burn Association 40:550-5572019Liquid Dermal Scaffold With Adipose-Derived Stem Cells Improve Tissue Quality in a Murine Model of Impaired Wound Healing.Applications:Unspecified applicationReactive species:Unspecified reactive speciesDiana Forbes,Breshell Russ,Ruhangiz Kilani,Aziz Ghahary,Reza JaliliPubMed 31188436
- Cell death & disease 9:4402018Seipin deficiency in mice causes loss of dopaminergic neurons via aggregation and phosphorylation of α-synuclein and neuroinflammation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLing Wang,Juan Hong,Yajuan Wu,George Liu,Wenfeng Yu,Ling ChenPubMed 29670081
- Proceedings of the National Academy of Sciences of 113:11883-118882016Dysregulation of Notch and ERα signaling in AhR-/- male mice.
- Proceedings of the National Academy of Sciences of 111:1933-82014Differential expression of estrogen receptor α, β1, and β2 in lobular and ductal breast cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBo Huang et. al.PubMed 24449868
- Proceedings of the National Academy of Sciences of 108:20090-42011Estrogen receptor β and 17β-hydroxysteroid dehydrogenase type 6, a growth regulatory pathway that is lost in prostate cancer.Applications:IHC-PReactive species:Unspecified reactive speciesSelvaraj Muthusamy et. al.PubMed 22114194
Key facts
- Host species
- Goat
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Conjugation
- Biotin
- Applications
- Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Dot | Reactivity Expected | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/98500 | Notes - |
Application IHC-Fr | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info - | Notes - |
Application IM | Reactivity Expected | Dilution info - | Notes - |
Application WB | Reactivity Expected | Dilution info 1/3000 - 1/30000 | Notes - |
Application ICC/IF | Reactivity Expected | Dilution info 1/500 - 1/3000 | Notes - |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF. Cited in 11 publications.
Key facts
- Description
- Goat Anti-Chicken IgY H&L (Biotin)
- Host species
- Goat
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Conjugation
- Biotin
- Applications
- Dot, ELISA, IHC-Fr, IHC-P, IM, WB, ICC/IF
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Purification technique
- Affinity purification
- Purification notes
Anti-Chicken IgG antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
This image is courtesy of a customer review submitted by Carl Hobbs.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)
ab89999 staining GFAP in rat spinal cord tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 10 minutes at 22°C; antigen retrieval was by heat mediation in citric acid. Samples were incubated with primary antibody (1/300 in TBS/BSA/Azide) for 2 hours at 22°C. A biotin-conjugated goat anti-chicken IgY H&L (ab6876) (1/100) was used as the secondary antibody.
Image from Yang Z et al., J Biol Chem. 2011 Apr 1;286(13):11444-55. Epub 2011 Jan 29. Fig 3.; doi: 10.1074/jbc.M110.212480; April 1, 2011, The Journal of Biological Chemistry, 286, 11444-11455.Immunohistochemistry (Frozen sections) - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)
Immunohistochemical analyis of Human lung tissue stained with a chicken primary antibody, followed by ab6876 at 1/500 dilution and horseradish peroxidase-conjugated streptavidin. Staining was detected using DAB.
ELISA - Goat Anti-Chicken IgY H&L (Biotin) (ab6876)
ELISA results of ab6876 against purified Chicken IgG. Each well was coated in duplicate with 1.0 µg of Chicken IgY (blue line). The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using Streptavidin HRP conjugate and TMB substrate.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com