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AB97133

Goat Anti-Chicken IgY H&L (Biotin)

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(1 Publication)

Suitable for ICC/IF, ELISA, IHC-P, WB. Cited in 1 publication.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G

1 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (AB97133)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (Biotin) (AB97133)

ab97133 was used at dilution 1/200 with the primary antibody ab81755 in IHC-P. See the review on ab81755.

Key facts

Host species

Goat

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Biotin

Excitation/Emission
Applications

ELISA, WB, ICC/IF, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/400000", "notes":"<p>(Primary).</p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/5000", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/20000 - 1/400000", "notes":"<p>(Chemiluminescent).</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Biotin. Biotinylated antibody was demonstrated with avidin/peroxidase when coated on microtiter wells.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

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Target data

See full target information Chicken IgY
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastroenterology 49:1453-66 PubMed24217965

2013

A gene expression profile related to immune dampening in the tumor microenvironment is associated with poor prognosis in gastric adenocarcinoma.

Applications

IHC

Species

Unspecified reactive species

Fatima Solange Pasini,Bruno Zilberstein,Igor Snitcovsky,Rosimeire Aparecida Roela,Flavia R Rotea Mangone,Ulysses Ribeiro,Suely Nonogaki,Glauber Costa Brito,Giovanna D Callegari,Ivan Cecconello,Venancio Avancini Ferreira Alves,José Eluf-Neto,Roger Chammas,Miriam Hatsue Honda Federico
View all publications
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