AB97145

Goat Anti-Chicken IgY H&L (Cy3 ®) preadsorbed

Name: Goat Anti Chicken (IgY) secondary antibody preadsorbed Cy3® (ab97145) | Abcam
Brand: Abcam Limited
SKU: AB97145
Price: 295 USD
Availability: InStock

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(12 Publications)

Suitable for IHC-P, Flow Cyt, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 12 publications.

Key facts

Host species

Goat

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Cow, Human, Pig, Mouse, Rat, Rabbit, Horse

Pre-adsorbed

Yes

Conjugation

Cy3®

Excitation/Emission

Ex: 552nm, Em: 565nm

Applications

ICC/IF, IHC-P, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/1000", "notes":"" }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy3 ®.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Chicken IgY

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

eLife 13: PubMed40407164

2025

Commissureless acts as a substrate adapter in a conserved Nedd4 E3 ubiquitin ligase pathway to promote axon growth across the midline.

Applications

Unspecified application

Species

Unspecified reactive species

Kelly G Sullivan,Greg J Bashaw

Science signaling 17:eadk2345 PubMed39353037

2024

A human DCC variant causing mirror movement disorder reveals that the WAVE regulatory complex mediates axon guidance by netrin-1-DCC.

Applications

Unspecified application

Species

Unspecified reactive species

Karina Chaudhari,Kaiyue Zhang,Patricia T Yam,Yixin Zang,Daniel A Kramer,Sarah Gagnon,Sabrina Schlienger,Sara Calabretta,Jean-Francois Michaud,Meagan Collins,Junmei Wang,Myriam Srour,Baoyu Chen,Frédéric Charron,Greg J Bashaw

Development (Cambridge, England) 151: PubMed38958007

2024

From promoter motif to cardiac function: a single DPE motif affects transcription regulation and organ function in vivo.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Sloutskin,Dekel Itzhak,Georg Vogler,Hadar Pozeilov,Diana Ideses,Hadar Alter,Orit Adato,Hadar Shachar,Tirza Doniger,Galit Shohat-Ophir,Manfred Frasch,Rolf Bodmer,Sascha H Duttke,Tamar Juven-Gershon

Science advances 8:eabn7450 PubMed36383665

2022

Clustering of vomeronasal receptor genes is required for transcriptional stability but not for choice.

Applications

Unspecified application

Species

Unspecified reactive species

Quentin Dietschi,Joël Tuberosa,Leon Fodoulian,Madlaina Boillat,Chenda Kan,Julien Codourey,Véronique Pauli,Paul Feinstein,Alan Carleton,Ivan Rodriguez

Frontiers in behavioral neuroscience 16:837523 PubMed35401134

2022

Circadian Rhythms and Sleep Are Dependent Upon Expression Levels of Key Ubiquitin Ligase .

Applications

Unspecified application

Species

Unspecified reactive species

Shu-Qun Shi,Carrie E Mahoney,Pavel Houdek,Wenling Zhao,Matthew P Anderson,Xinming Zhuo,Arthur Beaudet,Alena Sumova,Thomas E Scammell,Carl Hirschie Johnson

ASN neuro 13:17590914211044359 PubMed34618621

2021

Role of EphrinA3 in HIV-1 Neuropathogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Chitra Mohinder Singh Singal,Paritosh Jaiswal,Anuradha Mehta,Kanza Saleem,Pankaj Seth

Neuroendocrinology 112:384-398 PubMed34111876

2021

High Sensitivity of the Circadian Clock in the Hippocampal Dentate Gyrus to Glucocorticoid- and GSK3-Beta-Dependent Signals.

Applications

Unspecified application

Species

Unspecified reactive species

Karolína Liška,Martin Sládek,Pavel Houdek,Norzin Shrestha,Vendula Lužná,Martin R Ralph,Alena Sumová

eLife 10: PubMed33843588

2021

Robo recruitment of the Wave regulatory complex plays an essential and conserved role in midline repulsion.

Applications

Unspecified application

Species

Unspecified reactive species

Karina Chaudhari,Madhavi Gorla,Chao Chang,Artur Kania,Greg J Bashaw

The Journal of neuroscience : the official journal 39:3752-3769 PubMed30796158

2019

Translocator Protein Ligand Protects against Neurodegeneration in the MPTP Mouse Model of Parkinsonism.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Gong,Éva M Szego,Andrei Leonov,Eva Benito,Stefan Becker,Andre Fischer,Markus Zweckstetter,Tiago Outeiro,Anja Schneider

Cell 171:1354-1367.e20 PubMed29103614

2017

Membrane Microdomain Disassembly Inhibits MRSA Antibiotic Resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Esther García-Fernández,Gudrun Koch,Rabea M Wagner,Agnes Fekete,Stephanie T Stengel,Johannes Schneider,Benjamin Mielich-Süss,Sebastian Geibel,Sebastian M Markert,Christian Stigloher,Daniel Lopez

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Chicken IgY H&L (Cy3 ®) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Secondary Antibodies

Secondary Antibodies

Secondary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (12)

Product promise