AB97147

Goat Anti-Chicken IgY H&L (Cy5 ®) preadsorbed

Name: Goat Anti Chicken (IgY) secondary antibody preadsorbed Cy5® (ab97147) | Abcam
Brand: Abcam Limited
SKU: AB97147
Price: 295 USD
Availability: InStock
Rating: 5 (1 reviews)

(1 Review)

(10 Publications)

Suitable for ICC/IF, Flow Cyt, IHC-P. Preadsorbed to minimise non-specific binding and high background staining. Cited in 10 publications.

Key facts

Host species

Goat

Target species

Chicken

Target isotype

IgY

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Mouse, Rat, Rabbit, Horse, Cow, Human, Pig

Pre-adsorbed

Yes

Conjugation

Cy5®

Excitation/Emission

Ex: 650nm, Em: 667nm

Applications

ICC/IF, Flow Cyt, IHC-P

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgG and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Reduced cross-reactivity to bovine, goat, horse, human, mouse, pig, rabbit and rat was detected.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/1000", "notes":"" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antiserum was cross adsorbed using bovine, horse, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Cy5 ®.

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Chicken IgY

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Communications biology 8:480 PubMed40164758

2025

Protein covariation networks for elucidating ferroptosis inducer mechanisms and potential synergistic drug targets.

Applications

Unspecified application

Species

Unspecified reactive species

Rina Kunishige,Yoshiyuki Noguchi,Naomi Okamoto,Lei Li,Akito Ono,Masayuki Murata,Fumi Kano

Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism 42:104-120 PubMed34427142

2021

Short regulatory DNA sequences to target brain endothelial cells for gene therapy.

Applications

Unspecified application

Species

Unspecified reactive species

Hanna Graßhoff,Helge Müller-Fielitz,Godwin K Dogbevia,Jakob Körbelin,Jacqueline Bannach,Carl Mg Vahldieck,Kristina Kusche-Vihrog,Olaf Jöhren,Oliver J Müller,Ruben Nogueiras,Vincent Prevot,Markus Schwaninger

Biomaterials 194:47-56 PubMed30580195

2018

Contribution of bone marrow-derived cells to in situ engineered tissue capsules in a rat model of chronic kidney disease.

Applications

Unspecified application

Species

Unspecified reactive species

Taisiya Bezhaeva,Wouter J Geelhoed,Dong Wang,Haoyong Yuan,Eric P van der Veer,Carla M A van Alem,Febriyani F R Damanik,Xuefeng Qiu,Anton-Jan van Zonneveld,Lorenzo Moroni,Song Li,Joris I Rotmans

Human molecular genetics 28:1227-1243 PubMed30517687

2018

TUBA1A mutations identified in lissencephaly patients dominantly disrupt neuronal migration and impair dynein activity.

Applications

Unspecified application

Species

Unspecified reactive species

Jayne Aiken,Jeffrey K Moore,Emily A Bates

Nature nanotechnology 13:427-433 PubMed29610530

2018

Nanoparticle-induced neuronal toxicity across placental barriers is mediated by autophagy and dependent on astrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Simon J Hawkins,Lucy A Crompton,Aman Sood,Margaret Saunders,Noreen T Boyle,Amy Buckley,Aedín M Minogue,Sarah F McComish,Natalia Jiménez-Moreno,Oscar Cordero-Llana,Petros Stathakos,Catherine E Gilmore,Stephen Kelly,Jon D Lane,C Patrick Case,Maeve A Caldwell

The Journal of neuroscience : the official journal of the Society for Neuroscience 38:4275-4287 PubMed29610440

2018

Sustained Expression of Negative Regulators of Myelination Protects Schwann Cells from Dysmyelination in a Charcot-Marie-Tooth 1B Mouse Model.

Applications

Unspecified application

Species

Unspecified reactive species

Francesca Florio,Cinzia Ferri,Cristina Scapin,M Laura Feltri,Lawrence Wrabetz,Maurizio D'Antonio

Molecular metabolism 8:13-22 PubMed29254602

2017

The LepR-mediated leptin transport across brain barriers controls food reward.

Applications

IHC-P

Species

Mouse

Alessandro Di Spiezio,Elvira Sonia Sandin,Riccardo Dore,Helge Müller-Fielitz,Steffen E Storck,Mareike Bernau,Walter Mier,Henrik Oster,Olaf Jöhren,Claus U Pietrzik,Hendrik Lehnert,Markus Schwaninger

Nature communications 8:484 PubMed28883467

2017

Tanycytes control the hormonal output of the hypothalamic-pituitary-thyroid axis.

Applications

Unspecified application

Species

Unspecified reactive species

Helge Müller-Fielitz,Marcus Stahr,Mareike Bernau,Marius Richter,Sebastian Abele,Victor Krajka,Anika Benzin,Jan Wenzel,Kathrin Kalies,Jens Mittag,Heike Heuer,Stefan Offermanns,Markus Schwaninger

Developmental biology 424:147-161 PubMed28279709

2017

Vegf signaling promotes vascular endothelial differentiation by modulating etv2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Satish Casie Chetty,Megan S Rost,Jacob Ryan Enriquez,Jennifer A Schumacher,Kristina Baltrunaite,Andrea Rossi,Didier Y R Stainier,Saulius Sumanas

The Journal of endocrinology 226:93-102 PubMed26016747

2015

Effects of GnRH immunization on the reproductive axis and thymulin.

Applications

Unspecified application

Species

Unspecified reactive species

Shiping Su,Xiaoxia Sun,Xiuhong Zhou,Fuigui Fang,Yunsheng Li

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat Anti-Chicken IgY H&L (Cy5 ®) preadsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Complementary Products

Secondary Antibodies

Primary Antibodies

Secondary Antibodies

Secondary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Publications (10)

Product promise