Goat Anti-Chicken IgY H&L (HRP)

• WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

All lanes:

Western blot - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/cathepsin-d-antibody-epr3057y-chicken-igy-chimeric-ab302646'>ab302646</a>) at 1/1000 dilution

Lane 1:

Human heart tissue lysate at 20 µg

Lane 2:

Mouse brain tissue lysate at 20 µg

Lane 3:

Rat stomach tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/20000 dilution

Observed band size: 14 kDa,28 kDa,46 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Exposure time : 6 seconds

All lanes:

Western blot - Anti-IL-2 antibody [EPR20055] - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/il-2-antibody-epr20055-chicken-igy-chimeric-ab306565'>ab306565</a>) at 1/1000 dilution

All lanes:

Human IL-2 recombinant protein fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/10000 dilution

Observed band size: 17 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Blocking and diluting buffer and concentration : 5% NFDM/TBST. Negative control : spleen, colon (PMID : 10920202). Exposure times : Lanes 1-3 : 180 seconds Lanes 4-6 : 10 seconds Lanes 7-9 : 5 seconds

All lanes:

Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) - BSA and Azide free (<a href='/en-us/products/primary-antibodies/ddx4-mvh-antibody-epr24148-58-chicken-igy-chimeric-bsa-and-azide-free-ab307373'>ab307373</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate 10 µg

Lane 2:

Human spleen tissue lysate 20 µg

Lane 3:

Human colon tissue lysate 20 µg

Lane 4:

Mouse testis tissue lysate 10 µg

Lane 5:

Mouse colon tissue lysate 20 µg

Lane 6:

Mouse spleen tissue lysate 20 µg

Lane 7:

Rat testis tissue lysate 10 µg

Lane 8:

Rat colon tissue lysate 20 µg

Lane 9:

Rat spleen tissue lysate 20 µg

Observed band size: 79 kDa

false

Exposure time: 180s

• WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Exposure time : 5.5 seconds.

All lanes:

Western blot - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/cathepsin-d-antibody-epr3057y-chicken-igy-chimeric-ab302646'>ab302646</a>) at 1/1000 dilution

Lane 1:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

Lane 3:

SK-BR-3 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 4:

U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg

Lane 5:

A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 6:

HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

Lane 7:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 8:

Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/20000 dilution

Observed band size: 14 kDa,28 kDa,46 kDa

false

Exposure time: 5.5s

• WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Negative control : Daudi (PMID : 17606622).

In Western blot, ab316214 was shown to bind specifically to VIM (Vimentin). Target of interest was observed at 55 kDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in VIM (Vimentin) knockout cell line (lane 2, knockout cell line ab255446/ knockout cell lysate ab263775).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time : Lanes 1-4 : 8 seconds, Lanes 5 : 3 seconds, Lanes 6 : 10 seconds

All lanes:

Western blot - Anti-Vimentin antibody [EPR3776] - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/vimentin-antibody-epr3776-chicken-igy-chimeric-ab316214'>ab316214</a>) at 1/1000 dilution

Lane 1:

parental HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

VIM (Vimentin) knockout HeLa whole cell lysate at 20 µg

Lane 3:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 4:

Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

Lane 6:

C6 (rat glial tumor glial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/100000 dilution

Observed band size: 55 kDa,36 kDa

false

• WB

Ap

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139749 overnight at 4°C. Antibody binding was detected using an anti-chicken antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Western blot - Anti-NSE antibody (<a href='/en-us/products/primary-antibodies/nse-antibody-ab139749'>ab139749</a>) at 1 µg/mL

Lane 1:

Brain (Human) Cytoplasmic Lysate - fetal normal tissue at 10 µg

Lane 2:

SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 4:

Y79 (Human retinoblastoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

Predicted band size: 47 kDa

Observed band size: 50 kDa

true

Exposure time: 30s

• WB

Lab

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602).

Negative control : liver.

All lanes:

Western blot - Anti-GFAP antibody [EPR1034Y] - Astrocyte marker - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/gfap-antibody-epr1034y-astrocyte-marker-chicken-igy-chimeric-ab323239'>ab323239</a>) at 1/1000 dilution

Lane 1:

Human cerebellum tissue lysate at 20 µg

Lane 2:

Human liver tissue lysate at 20 µg

Lane 3:

Mouse brain tissue lysate at 20 µg

Lane 4:

Mouse liver tissue lysate at 20 µg

Lane 5:

Rat brain tissue lysate at 20 µg

Lane 6:

Rat liver tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/10000 dilution

Observed band size: 45-50 kDa

false

Exposure time: 3min

• WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Blocking / Diluting buffer : 5% NFDM/TBST Exposure time : 3.25 seconds

All lanes:

Western blot - Anti-Beclin-1 antibody [EPR19662] - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/beclin-1-antibody-epr19662-chicken-igy-chimeric-ab302669'>ab302669</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 2:

HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate 20 at 20 µg

Lane 3:

HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 4:

MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

Lane 5:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 6:

A20 (mouse reticum sarcoma B lymphocyte), whole cell lysate 20 at 20 µg

Lane 7:

C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg

Lane 8:

C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/20000 dilution

Observed band size: 52 kDa

false

Exposure time: 3.25s

• WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Negative control : kidney

In Western blot, Anti-Vinculin antibody [EPR8185] (ab129002) staining at 1/10000 dilution.

Exposure time : Lanes 1-2 : 10 seconds, Lanes 3-8 : 6 seconds

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-NEFH antibody [EPR20020] - Neuronal Marker - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/nefh-antibody-epr20020-neuronal-marker-chicken-igy-chimeric-ab317042'>ab317042</a>) at 1/1000 dilution

Lane 1:

Human Brainstem tissue lysate at 20 µg

Lane 2:

Human kidney tissue lysate at 20 µg

Lane 3:

Mouse cerebellum tissue lysate at 20 µg

Lane 4:

Mouse brain tissue lysate at 20 µg

Lane 5:

Mouse kidney tissue lysate at 20 µg

Lane 6:

Rat cerebellum tissue lysate at 20 µg

Lane 7:

Rat brain tissue lysate at 20 µg

Lane 8:

Rat kidney tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/100000 dilution

Observed band size: 200-210 kDa,124 kDa

false

• WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTIn Western blot, ab307839 was shown to bind specifically to NF-kB p65. A band was observed at 65 kDa in wild-type HAP1 cell lysates with whereas no signal observed at this size in NF-kB p65 knockout cell lysate. The samples were run on a Bis-Tris gel. In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution. Exposure time :

All lanes:

Western blot - Anti-NF-kB p65 antibody [E379] - Chicken IgY (Chimeric) - BSA and Azide free (<a href='/en-us/products/primary-antibodies/nf-kb-p65-antibody-e379-chicken-igy-chimeric-bsa-and-azide-free-ab307839'>ab307839</a>) at 1/1000 dilution

Lane 1:

Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell line) cell lysate at 20 µg

Lane 2:

NF-kB p65 knockout HAP1 cell lysate at 20 µg

Lane 3:

HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/20000 dilution

Observed band size: 65 kDa

false

Exposure time: 10s

• WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Blocking and diluting buffer and concentration : 5% NFDM/TBSTNegative control : spleen, colon (PMID : 10920202).

All lanes:

Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/ddx4-mvh-antibody-epr24148-58-chicken-igy-chimeric-ab314236'>ab314236</a>) at 1/1000 dilution

Lane 1:

Human testis tissue lysate at 10 µg

Lane 2:

Human spleen tissue lysate at 20 µg

Lane 3:

Human colon tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/100000 dilution

Observed band size: 79 kDa

false

Exposure time: 59s

• WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

The identity of higher MW band at approximately 100 kDa (in lane 1) is unknown.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Vimentin antibody [EPR3776] - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/vimentin-antibody-epr3776-chicken-igy-chimeric-ab316214'>ab316214</a>) at 1/1000 dilution

Lane 1:

Human lung tissue lysate at 20 µg

Lane 2:

Mouse lung tissue lysate at 20 µg

Lane 3:

Rat lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/100000 dilution

Observed band size: 55 kDa,36 kDa

false

Exposure time: 26s

• WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Blocking and diluting buffer and concentration : 5% NFDM/TBST Negative control : spleen, colon (PMID : 10920202).

All lanes:

Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/ddx4-mvh-antibody-epr24148-58-chicken-igy-chimeric-ab314236'>ab314236</a>) at 1/1000 dilution

Lane 1:

Mouse testis tissue lysate at 10 µg

Lane 2:

Mouse colon tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Lane 4:

Rat testis tissue lysate at 10 µg

Lane 5:

Rat colon tissue lysate at 20 µg

Lane 6:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/100000 dilution

Observed band size: 79 kDa

false

Exposure time: 10s

• WB

Ap

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab139744 overnight at 4°C. Antibody binding was detected using an anti-chicken antibody conjugated to HRP, and visualised using ECL development solution. The bands observed at 45- and 48-kDa correspond to the predicted molecular weights of isoforms CNPI and CNPII..

All lanes:

Western blot - Anti-CNPase antibody (<a href='/en-us/products/primary-antibodies/cnpase-antibody-ab139744'>ab139744</a>) at 1 µg/mL

Lane 1:

Spinal Cord (Mouse) Tissue Lysate at 10 µg

Lane 2:

Brain (Mouse) Tissue Lysate at 10 µg

Lane 3:

Spinal Cord (Rat) Tissue Lysate at 10 µg

Lane 4:

Brain (Rat) Tissue Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

Predicted band size: 48 kDa

Observed band size: 45 kDa,48 kDa

true

Exposure time: 90s

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

ab6877 was used at dilution 1/200 with the primary antibody ab17517 in IHC-P. See the review on ab17517.

• I-ELISA

Lab

Indirect ELISA - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Indirect ELISA showing primary antibody ab323001 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105), Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, ab272106) and Recombinant Human coronavirus SARS spike glycoprotein (Tagged, ab270844) at 1000 ng/ml. Binding of ab323001 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Chicken IgY H&L (HRP, ab6877) was used at 1 : 5000.

• I-ELISA

Lab

Indirect ELISA - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Indirect ELISA showing primary antibody ab322999 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, ab272105), Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, ab272106) and Recombinant Human coronavirus SARS spike glycoprotein (Tagged, ab270844) at 1000 ng/ml. Binding of ab322999 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Chicken IgY H&L (HRP, ab6877) was used at 1 : 5000.

• ELISA

Supplier Data

ELISA - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

ELISA results of ab6877 against purified Chicken IgG protein. Each well was coated in duplicate with 1.0 µg of Chicken IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1/97,900. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin and TMB substrate.

• WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Western blot analysis of ab6877 at 1 µg/mL. Secondary Antibody : Goat Anti-Chicken IgG HRP at 1/40000 dilution for 30 mins at RT.

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1 µg/mL

Lane 2:

RFP at 0.5 µg

Lane 3:

RFP at 0.1 µg

Lane 4:

HEK293T whole cell lysate spiked RFP at 10 µg

Secondary

All lanes:

Goat Anti-Chicken IgG HRP at 1/40000 dilution

false

• WB

Ap

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

ab73231 has a His tag so shows a high MW.

All lanes:

Western blot - Anti-PDGF BB antibody (<a href='/en-us/products/primary-antibodies/pdgf-bb-antibody-ab139753'>ab139753</a>) at 1 µg/mL

Lane 1:

Recombinant Human PDGF B protein (<a href='/en-us/products/unavailable/recombinant-human-pdgf-b-protein-ab73231'>ab73231</a>) at 0.01 µg

Lane 2:

Western blot - Recombinant human PDGF B protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pdgf-b-protein-ab9706'>ab9706</a>) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

Predicted band size: 27 kDa

Observed band size: 16.7 kDa

true

Exposure time: 8min

• WB

Ap

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

All lanes:

Western blot - Anti-PCNA antibody - Nuclear Loading Control (<a href='/en-us/products/primary-antibodies/pcna-antibody-nuclear-loading-control-ab139696'>ab139696</a>) at 1 µg/mL

Lane 1:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

Lane 3:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg

Lane 4:

Western blot - Recombinant Human PCNA protein (<a href='/en-us/products/proteins-peptides/recombinant-human-pcna-protein-ab85651'>ab85651</a>) at 0.2 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

Predicted band size: 29 kDa

Observed band size: 29 kDa

true

Exposure time: 4min

• WB

Project

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab134211 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

All lanes:

Anti-Histone H2B antibody (<a href='/en-us/products/unavailable/histone-h2b-antibody-ab134211'>ab134211</a>) at 1 µg/mL

Lane 1:

Calf Thymus Histone Preparation Nuclear Lysate at 0.2 µg

Lane 2:

HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

Lane 3:

HeLa Histone Preparation Nuclear Lysate at 2.5 µg

Lane 4:

Histone H2B Recombinant Protein at 20 µg

Lane 5:

Histone H3.1 Recombinant Protein at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

true

Exposure time: 2min

• WB

Lab

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

All lanes:

Anti-Oct4 antibody (ab134218) at 1 µg/mL

Lane 1:

E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg

Lane 2:

F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/10000 dilution

true

Exposure time: 1min

• WB

PubMed

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Representative Western blot analysis for the selected panel of differentially expressed targets in the various T cell subsets.

Western blot analyses were performed by serially probing sets of 3 membranes per donor. The expression levels of GDIR1, GSTO1, LIMS1 and PROF1 were analyzed blot 1 (upper panel), GDIR2, PRDX2 and THIO on blot 2 (middle panel) and SODM on blot 3 (lower level) of the respective membrane set.

The relative expression levels were defined by densitometric quantification normalized to the corresponding beta-actin signals, co-detected on each membrane.

Aliquots of 15 μg total lysate per sample/lane were loaded onto Tris-Tricine gels (16%T/2.5%C) and subsequently separated. Lysates generated form untreated CD45RA⁺ and CD45RO⁺ T cells were throughout the set of membranes loaded onto the lanes 1 and 3 respectively, whereas lysates generated from the corresponding T cell subsets exposure for 3 h to 5 μM H₂O₂ were loaded onto the lanes 2 and 4 as indicated.

The protein entry names of the targets (left) and their corresponding molecular weight (right) are listed.

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

false

Lichtenfels et al PLoS One. 2012;7(7):e41345. doi: 10.1371/journal.pone.0041345. Epub 2012 Jul 20. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

• WB

Lab

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

All lanes:

Western blot - Anti-STELLAR antibody (<a href='/en-us/products/primary-antibodies/stellar-antibody-ab136278'>ab136278</a>) at 1 µg/mL

Lane 1:

Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate at 20 µg

Lane 2:

Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate (ab59325) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

Predicted band size: 18 kDa

Observed band size: 20 kDa

true

Exposure time: 1min

• WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

Blocking / Diluting buffer and concentration : 5% NFDM/TBST Exposure time : 3.25 seconds

All lanes:

Western blot - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/gfp-antibody-epr14104-chicken-igy-chimeric-ab300643'>ab300643</a>) at 1/1000 dilution

Lane 1:

Non-transfected 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

GFP transfected 293 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/20000 dilution

Observed band size: 27 kDa

false

• WB

Supplier Data

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

In Western blot, anti-GAPDH antibody (ab181602) loading control staining.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) (<a href='/en-us/products/primary-antibodies/gfp-antibody-epr14104-chicken-igy-chimeric-ab300643'>ab300643</a>) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

293T cells transfected with an empty vector whole cell lysate at 20 µg

Lane 3:

293T cells transfected with GFP tagged IFNA2 protein whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/20000 dilution

Observed band size: 50 kDa

false

Exposure time: 40s

• WB

Unknown

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB6877)

ab6877 was used at dilution 1/1000 with the primary antibody ab25912 in WB. See the review on ab25912.

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/1000 dilution

false