Goat Anti-Chicken IgY H&L (HRP)

25 product images

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  All lanes: Western blot - Anti-STELLAR antibody (Anti-STELLAR antibody ab136278) at 1 µg/mL

  Lane 1: Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate at 20 µg

  Lane 2: Mouse EG (TMAS Embryonic Germ Cells) Whole Cell Lysate (ab59325) at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 18 kDa

  Observed band size: 20 kDa

  Exposure time: 1min

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  ab73231 has a His tag so shows a high MW.

  All lanes: Western blot - Anti-PDGF BB antibody (Anti-PDGF BB antibody ab139753) at 1 µg/mL

  Lane 1: Recombinant Human PDGF B protein (ab73231) at 0.01 µg

  Lane 2: Western blot - Recombinant human PDGF B protein (Recombinant human PDGF B protein ab9706) at 0.01 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 27 kDa

  Observed band size: 16.7 kDa

  Exposure time: 8min

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with Anti-CNPase antibody ab139744 overnight at 4°C. Antibody binding was detected using an anti-chicken antibody conjugated to HRP, and visualised using ECL development solution. The bands observed at 45- and 48-kDa correspond to the predicted molecular weights of isoforms CNPI and CNPII..

  All lanes: Western blot - Anti-CNPase antibody (Anti-CNPase antibody ab139744) at 1 µg/mL

  Lane 1: Spinal Cord (Mouse) Tissue Lysate at 10 µg

  Lane 2: Brain (Mouse) Tissue Lysate at 10 µg

  Lane 3: Spinal Cord (Rat) Tissue Lysate at 10 µg

  Lane 4: Brain (Rat) Tissue Lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 48 kDa

  Observed band size: 45 kDa, 48 kDa

  Exposure time: 90s

• 

  Lichtenfels et al PLoS One. 2012;7(7):e41345. doi: 10.1371/journal.pone.0041345. Epub 2012 Jul 20. Fig 4. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Representative Western blot analysis for the selected panel of differentially expressed targets in the various T cell subsets.

  Western blot analyses were performed by serially probing sets of 3 membranes per donor. The expression levels of GDIR1, GSTO1, LIMS1 and PROF1 were analyzed blot 1 (upper panel), GDIR2, PRDX2 and THIO on blot 2 (middle panel) and SODM on blot 3 (lower level) of the respective membrane set.

  The relative expression levels were defined by densitometric quantification normalized to the corresponding beta-actin signals, co-detected on each membrane.

  Aliquots of 15 μg total lysate per sample/lane were loaded onto Tris-Tricine gels (16%T/2.5%C) and subsequently separated. Lysates generated form untreated CD45RA⁺ and CD45RO⁺ T cells were throughout the set of membranes loaded onto the lanes 1 and 3 respectively, whereas lysates generated from the corresponding T cell subsets exposure for 3 h to 5 μM H₂O₂ were loaded onto the lanes 2 and 4 as indicated.

  The protein entry names of the targets (left) and their corresponding molecular weight (right) are listed.

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  All lanes: Western blot - Anti-PCNA antibody - Nuclear Loading Control (Anti-PCNA antibody - Nuclear Loading Control ab139696) at 1 µg/mL

  Lane 1: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg

  Lane 2: HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 20 µg

  Lane 3: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate (ab27252) at 20 µg

  Lane 4: Western blot - Recombinant Human PCNA protein (Recombinant Human PCNA protein ab85651) at 0.2 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 29 kDa

  Observed band size: 29 kDa

  Exposure time: 4min

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with Anti-NSE antibody ab139749 overnight at 4°C. Antibody binding was detected using an anti-chicken antibody conjugated to HRP, and visualised using ECL development solution.

  All lanes: Western blot - Anti-NSE antibody (Anti-NSE antibody ab139749) at 1 µg/mL

  Lane 1: Brain (Human) Cytoplasmic Lysate - fetal normal tissue at 10 µg

  Lane 2: SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate at 10 µg

  Lane 3: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 4: Y79 (Human retinoblastoma cell line) Whole Cell Lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Predicted band size: 47 kDa

  Observed band size: 50 kDa

  Exposure time: 30s

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  ab6877 was used at dilution 1/1000 with the primary antibody ab25912 in WB. See the review on ab25912.

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  All lanes: Anti-Oct4 antibody (ab134218) at 1 µg/mL

  Lane 1: E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate at 10 µg

  Lane 2: F9 (Mouse embryonic carcinoma cell line) Whole Cell Lysate at 10 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/10000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Exposure time: 1min

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab134211 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

  All lanes: Anti-Histone H2B antibody (ab134211) at 1 µg/mL

  Lane 1: Calf Thymus Histone Preparation Nuclear Lysate at 0.2 µg

  Lane 2: HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 10 µg

  Lane 3: HeLa Histone Preparation Nuclear Lysate at 2.5 µg

  Lane 4: Histone H2B Recombinant Protein at 20 µg

  Lane 5: Histone H3.1 Recombinant Protein at 0.1 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/3000 dilution

  Developed using the ECL technique.

  Performed under reducing conditions.

  Exposure time: 2min

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Exposure time: 5.5 seconds.

  All lanes: Western blot - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) ab302646) at 1/1000 dilution

  Lane 1: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

  Lane 2: SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate at 20 µg

  Lane 3: SK-BR-3 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

  Lane 4: U937 (human histiocytic lymphoma monocyte), whole cell lysate at 20 µg

  Lane 5: A431 (human epidermoid carcinoma epithelial cell), whole cell lysate at 20 µg

  Lane 6: HepG2 (human hepatocellular carcinoma epithelial cell), whole cell lysate at 20 µg

  Lane 7: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

  Lane 8: Neuro-2a (mouse neuroblastoma neuroblast), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/20000 dilution

  Observed band size: 14 kDa, 28 kDa, 46 kDa

  Exposure time: 5.5s

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  Exposure time: 10 seconds.

  All lanes: Western blot - Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) (Anti-Cathepsin D antibody [EPR3057Y] - Chicken IgY (Chimeric) ab302646) at 1/1000 dilution

  Lane 1: Human heart tissue lysate at 20 µg

  Lane 2: Mouse brain tissue lysate at 20 µg

  Lane 3: Rat stomach tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/20000 dilution

  Observed band size: 14 kDa, 28 kDa, 46 kDa

  Exposure time: 10s

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  The identity of higher MW band at approximately 100 kDa (in lane 1) is unknown.

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  All lanes: Western blot - Anti-Vimentin antibody [EPR3776] - Chicken IgY (Chimeric) (Anti-Vimentin antibody [EPR3776] - Chicken IgY (Chimeric) ab316214) at 1/1000 dilution

  Lane 1: Human lung tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 2: Mouse lung tissue lysate at 20 µg with 5% NFDM/TBST

  Lane 3: Rat lung tissue lysate at 20 µg with 5% NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/100000 dilution

  Observed band size: 55 kDa, 36 kDa

  Exposure time: 26s

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Negative control: Daudi (PMID: 17606622).

  In Western blot, Anti-Vimentin antibody [EPR3776] - Chicken IgY (Chimeric) ab316214 was shown to bind specifically to VIM (Vimentin). Target of interest was observed at 55 kDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in VIM (Vimentin) knockout cell line (lane 2, knockout cell line Human VIM (Vimentin) knockout HeLa cell line ab255446/ knockout cell lysate Human VIM (Vimentin) knockout HeLa cell lysate ab263775).

  In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

  Exposure time: Lanes 1-4: 8 seconds, Lanes 5: 3 seconds, Lanes 6: 10 seconds

  All lanes: Western blot - Anti-Vimentin antibody [EPR3776] - Chicken IgY (Chimeric) (Anti-Vimentin antibody [EPR3776] - Chicken IgY (Chimeric) ab316214) at 1/1000 dilution

  Lane 1: parental HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 2: VIM (Vimentin) knockout HeLa whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 3: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 4: Daudi (human Burkitts lymphoma lymphoblast) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 5: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg with 5% NFDM/TBST

  Lane 6: C6 (rat glial tumor glial cell) whole cell lysate at 20 µg with 5% NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/100000 dilution

  Performed under reducing conditions.

  Observed band size: 55 kDa, 36 kDa

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Blocking / Diluting buffer: 5% NFDM/TBST
  
  Exposure time: 3.25 seconds

  All lanes: Western blot - Anti-Beclin-1 antibody [EPR19662] - Chicken IgY (Chimeric) (Anti-Beclin-1 antibody [EPR19662] - Chicken IgY (Chimeric) ab302669) at 1/1000 dilution

  Lane 1: HeLa (human cervix adenocarcinoma epithelial cell), whole cell lysate at 20 µg

  Lane 2: HepG2 (human hepatocellar carcinoma epithelial cell), whole cell lysate 20 at 20 µg

  Lane 3: HEK-293 (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

  Lane 4: MCF7 (human breast adenocarcinoma epithelial cell), whole cell lysate at 20 µg

  Lane 5: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

  Lane 6: A20 (mouse reticum sarcoma B lymphocyte), whole cell lysate 20 at 20 µg

  Lane 7: C2C12 (mouse myoblasts myoblast), whole cell lysate at 20 µg

  Lane 8: C6 (rat glial tumor glial cell), whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/20000 dilution

  Observed band size: 52 kDa

  Exposure time: 3.25s

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  Negative control: spleen, colon (PMID: 10920202).

  All lanes: Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) ab314236) at 1/1000 dilution

  Lane 1: Mouse testis tissue lysate at 10 µg

  Lane 2: Mouse colon tissue lysate at 20 µg

  Lane 3: Mouse spleen tissue lysate at 20 µg

  Lane 4: Rat testis tissue lysate at 10 µg

  Lane 5: Rat colon tissue lysate at 20 µg

  Lane 6: Rat spleen tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/100000 dilution

  Observed band size: 79 kDa

  Exposure time: 10s

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Negative control: kidney

  In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.

  Exposure time: Lanes 1-2: 10 seconds, Lanes 3-8: 6 seconds

  All lanes: Western blot - Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) (Anti-NEFH antibody [EPR20020] - Chicken IgY (Chimeric) ab317042) at 1/1000 dilution

  Lane 1: Human Brainstem tissue lysate at 20 µg with NFDM/TBST

  Lane 2: Human kidney tissue lysate at 20 µg with NFDM/TBST

  Lane 3: Mouse cerebellum tissue lysate at 20 µg with NFDM/TBST

  Lane 4: Mouse brain tissue lysate at 20 µg with NFDM/TBST

  Lane 5: Mouse kidney tissue lysate at 20 µg with NFDM/TBST

  Lane 6: Rat cerebellum tissue lysate at 20 µg with NFDM/TBST

  Lane 7: Rat brain tissue lysate at 20 µg with NFDM/TBST

  Lane 8: Rat kidney tissue lysate at 20 µg with NFDM/TBST

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/100000 dilution

  Observed band size: 200-210 kDa, 124 kDa

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Blocking and diluting buffer and concentration: 5% NFDM/TBSTNegative control: spleen, colon (PMID: 10920202).

  All lanes: Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) (Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) ab314236) at 1/1000 dilution

  Lane 1: Human testis tissue lysate at 10 µg

  Lane 2: Human spleen tissue lysate at 20 µg

  Lane 3: Human colon tissue lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/100000 dilution

  Observed band size: 79 kDa

  Exposure time: 59s

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Blocking / Diluting buffer and concentration: 5% NFDM/TBST
  
  Exposure time: 3.25 seconds

  All lanes: Western blot - Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) (Anti-GFP antibody [EPR14104] - Chicken IgY (Chimeric) ab300643) at 1/1000 dilution

  Lane 1: Non-transfected 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

  Lane 2: GFP transfected 293 whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/20000 dilution

  Observed band size: 27 kDa

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST
  
  Exposure time: 6 seconds

  All lanes: Western blot - Anti-IL-2 antibody [EPR20055] - Chicken IgY (Chimeric) (Anti-IL-2 antibody [EPR20055] - Chicken IgY (Chimeric) ab306565) at 1/1000 dilution

  All lanes: Human IL-2 recombinant protein fragment at 10 ng

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/10000 dilution

  Observed band size: 17 kDa

  Exposure time: 6s

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Blocking and diluting buffer and concentration: 5% NFDM/TBSTIn Western blot, Anti-NF-kB p65 antibody [E379] - Chicken IgY (Chimeric) - BSA and Azide free ab307839 was shown to bind specifically to NF-kB p65. A band was observed at 65 kDa in wild-type HAP1 cell lysates with whereas no signal observed at this size in NF-kB p65 knockout cell lysate.
  The samples were run on a Bis-Tris gel.
  In Western blot, anti-GAPDH antibody (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) loading control staining at 1/200000 dilution.
  Exposure time:

  All lanes: Western blot - Anti-NF-kB p65 antibody [E379] - Chicken IgY (Chimeric) - BSA and Azide free (Anti-NF-kB p65 antibody [E379] - Chicken IgY (Chimeric) - BSA and Azide free ab307839) at 1/1000 dilution

  Lane 1: Wild-type HAP1 (human chronic myelogenous leukemia near-haploid cell line) cell lysate at 20 µg

  Lane 2: NF-kB p65 knockout HAP1 cell lysate at 20 µg

  Lane 3: HeLa (human epithelial cell line from cervical adenocarcinoma) whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1/20000 dilution

  Observed band size: 65 kDa

  Exposure time: 10s

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.
  
  Negative control: spleen, colon (PMID: 10920202).
  
  Exposure times:
  
  Lanes 1-3: 180 seconds
  
  Lanes 4-6: 10 seconds
  
  Lanes 7-9: 5 seconds

  All lanes: Western blot - Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) - BSA and Azide free (Anti-DDX4 / MVH antibody [EPR24148-58] - Chicken IgY (Chimeric) - BSA and Azide free ab307373) at 1/1000 dilution

  Lane 1: Human testis tissue lysate 10 µg

  Lane 2: Human spleen tissue lysate 20 µg

  Lane 3: Human colon tissue lysate 20 µg

  Lane 4: Mouse testis tissue lysate 10 µg

  Lane 5: Mouse colon tissue lysate 20 µg

  Lane 6: Mouse spleen tissue lysate 20 µg

  Lane 7: Rat testis tissue lysate 10 µg

  Lane 8: Rat colon tissue lysate 20 µg

  Lane 9: Rat spleen tissue lysate 20 µg

  Observed band size: 79 kDa

  Exposure time: 180s

• 

  Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Western blot analysis of ab6877 at 1 µg/mL. Secondary Antibody: Goat Anti-Chicken IgG HRP at 1/40000 dilution for 30 mins at RT.

  All lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab6877) at 1 µg/mL

  Lane 2: RFP at 0.5 µg

  Lane 3: RFP at 0.1 µg

  Lane 4: HEK293T whole cell lysate spiked RFP at 10 µg

  Secondary

  All lanes: Goat Anti-Chicken IgG HRP at 1/40000 dilution

• 

  Indirect ELISA - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Indirect ELISA showing primary antibody Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Chicken IgY (Chimeric) ab323001 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105), Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) ab272106) and Recombinant Human coronavirus SARS spike glycoprotein (Tagged, Recombinant Human coronavirus SARS spike glycoprotein (Tagged) ab270844) at 1000 ng/ml. Binding of Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [EPR24852-116] - Chicken IgY (Chimeric) ab323001 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Chicken IgY H&L (HRP, ab6877) was used at 1:5000.

• 

  Indirect ELISA - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  Indirect ELISA showing primary antibody Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - Chicken IgY (Chimeric) ab322999 binding to Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105). Plates were coated with Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S1 (Fc Chimera) ab272105), Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera, Recombinant Human coronavirus SARS-CoV-2 Spike Glycoprotein S2 (Fc Chimera) ab272106) and Recombinant Human coronavirus SARS spike glycoprotein (Tagged, Recombinant Human coronavirus SARS spike glycoprotein (Tagged) ab270844) at 1000 ng/ml. Binding of Anti-SARS-CoV-2 Spike Glycoprotein S1 antibody [8B12-C2] - Chicken IgY (Chimeric) ab322999 was assessed in a serial dilution range 0.016- 1000 ng/mL (a 3-fold serial dilution). Secondary antibody, Goat Anti-Chicken IgY H&L (HRP, ab6877) was used at 1:5000.

• 

  ELISA - Goat Anti-Chicken IgY H&L (HRP) (ab6877)

  ELISA results of ab6877 against purified Chicken IgG protein. Each well was coated in duplicate with 1.0 µg of Chicken IgG. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. The titer is 1/97,900. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gelatin and TMB substrate.