Goat Anti-Chicken IgY H&L (HRP)

• WB

AbReview34401****

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB97135)

All lanes:

Western blot - Anti-Vimentin antibody (<a href='/en-us/products/primary-antibodies/vimentin-antibody-ab24525'>ab24525</a>)

All lanes:

Mouse cardiac fibroblasts at 4 µg

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135) at 1/2500 dilution

true

Exposure time: 2s

This image is courtesy of an anonymous Abreview.

• WB

Unknown

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB97135)

ab97135 used to detect a chicken anti-beta Galactosidase antibody (ab9361). ab97135 used at a concentration of 1 : 2000 in 5% milk blocking buffer in 0.1% Tween, 1x PBS. Whole lysates from DH5a (E.coli). Specific band at approx. 110 KDa. Non-specific bands : 70 Kda. NB. Gels run on 10% Acrylamide gels.

This image is courtesy of an anonymous Abreview.

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135)

false

• WB

Unknown

Western blot - Goat Anti-Chicken IgY H&L (HRP) (AB97135)

All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 μg/ml

Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 μg per lane.

Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/2000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/10000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/20000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Exposure time : 90 seconds

Secondary

All lanes:

Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135)

false