Suitable for ICC, WB, IHC-P, ELISA. Ideal for western blot. Cited in 37 publications.
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- PloS one 18:e02926812023TAT peptide at treatment-level concentrations crossed brain endothelial cell monolayer independent of receptor-mediated endocytosis or peptide-inflicted barrier disruption.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMeng-Chih Wu,Eric Yuhsiang Wang,Ted Weita LaiPubMed 37819924
- Nature communications 14:45042023Pharmacological perturbation of the phase-separating protein SMNDC1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLennart Enders,Marton Siklos,Jan Borggräfe,Stefan Gaussmann,Anna Koren,Monika Malik,Tatjana Tomek,Michael Schuster,Jiří Reiniš,Elisa Hahn,Andrea Rukavina,Andreas Reicher,Tamara Casteels,Christoph Bock,Georg E Winter,J Thomas Hannich,Michael Sattler,Stefan KubicekPubMed 37587144
- Cells 12:2023Innate Immune Status of Glia Modulates Prion Propagation in Early Stage of Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSang-Gyun Kang,Chiye Kim,Judd Aiken,Debbie McKenziePubMed 37508542
- Frontiers in cellular neuroscience 17:11583882023SARM1 detection in myelinating glia: / is dispensable for PNS and CNS myelination in zebrafish and mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesShaline V Fazal,Clara Mutschler,Civia Z Chen,Mark Turmaine,Chiung-Ya Chen,Yi-Ping Hsueh,Andrea Ibañez-Grau,Andrea Loreto,Angeles Casillas-Bajo,Hugo Cabedo,Robin J M Franklin,Roger A Barker,Kelly R Monk,Benjamin J Steventon,Michael P Coleman,Jose A Gomez-Sanchez,Peter Arthur-FarrajPubMed 37091921
- eNeuro 9:2022Blood-Brain Barrier Disruption in Preclinical Mouse Models of Stroke Can Be an Experimental Artifact Caused by Craniectomy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChe-Wei Liu,Eric Yuhsiang Wang,Hwai-Lee Wang,Kate Hsiurong Liao,Hsiao-Yun Chen,Hank Szuhan Chen,Ted Weita LaiPubMed 36224001
- Free radical biology & medicine 188:175-1842022Extracellular biomolecular free radical formation during injury.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMadeline R Hines,Jessica E Goetz,Piedad C Gomez-Contreras,Samuel N Rodman,Suryamin Liman,Elise L Femino,Paige N Kluz,Brett A Wagner,Garry R Buettner,Eric E Kelley,Mitchell C ColemanPubMed 35724853
- Journal of basic microbiology 62:669-6882022The Escherichia coli DnaK chaperone stimulates the α-complementation of β-galactosidase.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSamuel Berhanu,Takuya Ueda,Jean-Hervé AlixPubMed 35289419
- Cellular and molecular life sciences : CMLS 79:482021Activation of neutral sphingomyelinase 2 through hyperglycemia contributes to endothelial apoptosis via vesicle-bound intercellular transfer of ceramides.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAndreas Zietzer,Alina Lisann Jahnel,Marko Bulic,Katharina Gutbrod,Philip Düsing,Mohammed Rabiul Hosen,Peter Dörmann,Nikos Werner,Georg Nickenig,Felix JansenPubMed 34951654
- mAbs 13:19970722021Hydrogen-deuterium exchange mass spectrometry reveals three unique binding responses of mAbs directed to the catalytic domain of hCAIX.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJoey G Sheff,John F Kelly,Anna Robotham,Traian Sulea,Félix Malenfant,Denis L'Abbé,Mélanie Duchesne,Alex Pelletier,Jean Lefebvre,Andrea Acel,Marie Parat,Mylene Gosselin,Cunle Wu,Yves Fortin,Jason Baardsnes,Henk Van Faassen,Shannon Awrey,Shawn C Chafe,Paul C McDonald,Shoukat Dedhar,Anne E G LenferinkPubMed 34812124
- Human molecular genetics 31:705-7172021α-synuclein inhibits Snx3-retromer retrograde trafficking of the conserved membrane-bound proprotein convertase Kex2 in the secretory pathway of Saccharomyces cerevisiae.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSanthanasabapathy Rajasekaran,Patricia P Peterson,Zhengchang Liu,Lucy C Robinson,Stephan N WittPubMed 34570221
Key facts
- Host species
- Goat
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- ICC, WB, IHC-P, ELISA
- Clonality
- Polyclonal
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info 1/2000.00000 - 1/20000.00000 | Notes Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000 |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/5000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/100000.00000 | Notes (Primary). |
Target data
Alternative names
Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma-2 chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G
Recommended products
Suitable for ICC, WB, IHC-P, ELISA. Ideal for western blot. Cited in 37 publications.
Key facts
- Description
- Goat Anti-Chicken IgY H&L (HRP)
- Host species
- Goat
- Target species
- Chicken
- Target isotype
- IgY
- Target specificity
- Heavy & Light chains
- Conjugation
- HRP
- Applications
- ICC, WB, IHC-P, ELISA
- Clonality
- Polyclonal
- Pre-adsorbed
- No
- Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Chicken IgY and with light chains common to other Chicken immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
Part of the AbExcel range.
Product promise
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Full details and terms and conditions can be found here:
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3 product images
This image is courtesy of an anonymous customer review.Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135)
All lanes: Western blot - Anti-Vimentin antibody (Anti-Vimentin antibody ab24525)
All lanes: Mouse cardiac fibroblasts at 4 µg
SecondaryAll lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135) at 1/2500 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 2s
Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135)
All lanes : Anti-beta Actin antibody - Loading Control (ab13822) at 1 μg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 μg per lane.
Secondary
Lanes 1 - 2 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/2000 dilution
Lanes 3 - 4 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/10000 dilution
Lanes 5 - 6 : Goat polyclonal Secondary Antibody to Chicken IgY - H&L (HRP) (ab97135) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time : 90 secondsSecondaryAll lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135)
Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135)
ab97135 used to detect a chicken anti-beta Galactosidase antibody (Anti-beta Galactosidase antibody ab9361). ab97135 used at a concentration of 1:2000 in 5% milk blocking buffer in 0.1% Tween, 1x PBS. Whole lysates from DH5a (E.coli). Specific band at approx. 110 KDa. Non-specific bands: 70 Kda. NB. Gels run on 10% Acrylamide gels.
This image is courtesy of an anonymous Abreview.
SecondaryAll lanes: Western blot - Goat Anti-Chicken IgY H&L (HRP) (ab97135)
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