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Goat anti-mouse IgG H&L is a F(ab) fragment secondary antibody. Suitable for western blot, dot blot, IHC, ICC/IF and ELISA.

- Cited in over 30 publications

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Images

SDS-PAGE - Goat F(ab) Anti-Mouse IgG H&L (AB6668), expandable thumbnail
  • Dot Blot - Goat F(ab) Anti-Mouse IgG H&L (AB6668), expandable thumbnail

Publications

Key facts

Host species
Goat
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Applications
WB, ELISA, IHC, ICC/IF, Dot
Clonality
Polyclonal
Format
F(ab)

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Reactivity data

Application
WB
Reactivity
Reacts
Dilution info
1/2000.00000 - 1/10000.00000
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/15000
Notes

-

Application
IHC
Reactivity
Reacts
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/1000.00000 - 1/5000.00000
Notes

-

Application
Dot
Reactivity
Expected
Dilution info
-
Notes

-

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Alternative names

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Goat anti-mouse IgG H&L is a F(ab) fragment secondary antibody. Suitable for western blot, dot blot, IHC, ICC/IF and ELISA.

- Cited in over 30 publications

Key facts

Description
Goat F(ab) Anti-Mouse IgG H&L
Host species
Goat
Target species
Mouse
Target isotype
IgG
Target specificity
Heavy & Light chains
Applications
WB, ELISA, IHC, ICC/IF, Dot
Clonality
Polyclonal
Format
F(ab)
Pre-adsorbed
No
Isotype
IgG
Concentration
Loading...

Properties

Form
Liquid
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Purification technique
Affinity purification
Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

When it comes to advancing your immunohistochemistry (IHC) research, Abcam offers comprehensive suite of IHC kits and secondary antibodies , designed to deliver precise and reliable results.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • SDS-PAGE - Goat F(ab) Anti-Mouse IgG H&L (ab6668), expandable thumbnail

    SDS-PAGE - Goat F(ab) Anti-Mouse IgG H&L (ab6668)

    SDS-PAGE results of Goat Fab Anti-Mouse IgG (H&L) ab6668.

    Lane 1: Non-reduced Goat Fab Anti-Mouse.

    Lane 2: Opal Prestained Ladder.

    Lane 3: Reduced Goat Fab Anti-Mouse.

    Load: 1.0 μg.

    Coomassie stained and imaged.

  • Dot Blot - Goat F(ab) Anti-Mouse IgG H&L (ab6668), expandable thumbnail

    Dot Blot - Goat F(ab) Anti-Mouse IgG H&L (ab6668)

    Dot Blot results of Goat Fab Anti-Mouse IgG Antibody ab6668.

    Dots are Mouse IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng.

    Blocked for 30 min at RT.

    Primary Antibody: Goat Fab Anti-Mouse IgG at 1 μg/mL for 1 hr at RT.

    Secondary Antibody: Donkey Anti-Goat IgG HRP at 1:40,000 for 30 min at RT.

    Imaged with BioRad ChemiDoc, Chemi filter.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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