Goat anti-mouse IgG H&L is a F(ab) fragment secondary antibody. Suitable for western blot, dot blot, IHC, ICC/IF and ELISA.
- Cited in over 30 publications
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application WB | Reactivity Reacts | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/15000 | Notes - |
Application IHC | Reactivity Reacts | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Application ICC/IF | Reactivity Reacts | Dilution info 1/1000.00000 - 1/5000.00000 | Notes - |
Application Dot | Reactivity Expected | Dilution info - | Notes - |
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Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Goat anti-mouse IgG H&L is a F(ab) fragment secondary antibody. Suitable for western blot, dot blot, IHC, ICC/IF and ELISA.
- Cited in over 30 publications
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
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Lane 1: Non-reduced Goat Fab Anti-Mouse.
Lane 2: Opal Prestained Ladder.
Lane 3: Reduced Goat Fab Anti-Mouse.
Load: 1.0 μg.
Coomassie stained and imaged.
Dots are Mouse IgG at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng.
Blocked for 30 min at RT.
Primary Antibody: Goat Fab Anti-Mouse IgG at 1 μg/mL for 1 hr at RT.
Secondary Antibody: Donkey Anti-Goat IgG HRP at 1:40,000 for 30 min at RT.
Imaged with BioRad ChemiDoc, Chemi filter.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
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