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AB7055

Goat F(ab) Anti-Rabbit IgG H&L (Biotin)

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(2 Publications)

Suitable for WB, Dot, IM, IHC, ICC/IF, ELISA. Cited in 2 publications.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

1 Images
ELISA - Goat F(ab) Anti-Rabbit IgG H&L (Biotin) (AB7055)
  • ELISA

Supplier Data

ELISA - Goat F(ab) Anti-Rabbit IgG H&L (Biotin) (AB7055)

ELISA results of ab7055 Each well was coated in duplicate with 10 µg of Rabbit IgG. The working dilution is 368,000. The starting dilution of antibody was 5 µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% Fish Gel, Streptavidin-HRP conjugated, and TMB substrate.

  • Unconjugated

    Goat F(ab) Anti-Rabbit IgG H&L

  • HRP

    Goat F(ab) Anti-Rabbit IgG H&L (HRP)

  • 519 FITC

    Goat F(ab) Anti-Rabbit IgG H&L (FITC)

  • 572 TRITC

    Goat F(ab) Anti-Rabbit IgG H&L (TRITC)

  • 615 Texas Red®

    Goat F(ab) Anti-Rabbit IgG H&L (Texas Red ®)

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity
Pre-adsorbed

No

Conjugation

Biotin

Excitation/Emission
Applications

ELISA, Dot, IHC, IM, WB, ICC/IF

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against anti-Papain or anti-Goat IgG F(c).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"<p></p>" }, "IHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/1000 - 1/5000", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/90000", "notes":"<p></p>" } } }
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We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
Storage buffer
Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Rabbit IgG
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of periodontal research 59:128-139 PubMed37947055

2023

Interleukin-37 ameliorates periodontitis development by inhibiting NLRP3 inflammasome activation and modulating M1/M2 macrophage polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Liyan Yang,Wei Tao,Chen Xie,Qiuye Chen,Yunshan Zhao,Li Zhang,Xu Xiao,Shilu Wang,Xu Zheng

Oncoimmunology 10:1940675 PubMed34290905

2021

T cells drive negative feedback mechanisms in cancer associated fibroblasts, promoting expression of co-inhibitory ligands, CD73 and IL-27 in non-small cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Richard A O'Connor,Vishwani Chauhan,Layla Mathieson,Helen Titmarsh,Lilian Koppensteiner,Irene Young,Guilia Tagliavini,David A Dorward,Sandrine Prost,Kevin Dhaliwal,William A Wallace,Ahsan R Akram
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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