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Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 10 publications.
Alternative names=Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Goat
Human
IgG
F(ab')2
HRP
ICC, IHC-P, ELISA, WB
Polyclonal
F(ab')2
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application ICC | Reactivity Reacts | Dilution info - | Notes - |
Application IHC-P | Reactivity Reacts | Dilution info 1/200.00000 - 1/2000.00000 | Notes - |
Application ELISA | Reactivity Reacts | Dilution info 1/10000.00000 - 1/100000.00000 | Notes (Primary). |
Application WB | Reactivity Reacts | Dilution info 1/2000.00000 - 1/25000.00000 | Notes (Colorimetric: 1/2000 - 1/20000, Chemiluminescent: 1/5000 - 1/25000). |
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).
Suitable for ICC, IHC-P, ELISA, WB. Ideal for western blot. Cited in 10 publications.
Alternative names=Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Goat F(ab')2 Anti-Human IgG (Fab')2 (HRP)
Goat
Human
IgG
F(ab')2
HRP
ICC, IHC-P, ELISA, WB
Polyclonal
F(ab')2
No
IgG
Liquid
pH: 6.8 - 7.4
Preservative: 0.05% CMIT/MIT based preservative
Constituents: 0.2% BSA
Affinity purification Immunogen
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Horse Radish Peroxidase.
Blue Ice
+4°C
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