Goat F(ab')2 Anti-Human IgG - Fc (PE) is a pre-adsorbed secondary antibody with a maximum absorption wavelength of 480;565nm and a maximum emission wavelength of 578nm. Suitable for Flow Cytometry.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- F(ab) fragment secondary antibodies eliminate non-specific binding between Fc regions of antibodies and Fc receptors on immune cells
- Cited in over 10 publications
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- Phytomedicine : international journal of phytotherapy and phytopharmacology 118:1549422023Kaempferol inhibits SARS-CoV-2 invasion by impairing heptad repeats-mediated viral fusion.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJunwei Gao,Can Cao,Mingfei Shi,Shihao Hong,Shijie Guo,Jing Li,Tengxiao Liang,Ping Song,Ruodan Xu,Ning LiPubMed 37421767
- Journal for immunotherapy of cancer 10:2022Antibody-based redirection of universal Fabrack-CAR T cells selectively kill antigen bearing tumor cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYi-Chiu Kuo,Cheng-Fu Kuo,Kurt Jenkins,Alfur Fu-Hsin Hung,Wen-Chung Chang,Miso Park,Brenda Aguilar,Renate Starr,Jonathan Hibbard,Christine Brown,John C WilliamsPubMed 35728874
- iScience 25:1037202022ACE2 can act as the secondary receptor in the FcγR-dependent ADE of SARS-CoV-2 infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZai Wang,Tingting Deng,Yulian Zhang,Wenquan Niu,Qiangqiang Nie,Shengnan Yang,Peipei Liu,Pengfei Pei,Long Chen,Haibo Li,Bin CaoPubMed 35005526
- Cell reports 35:1091422021Inhibitory affinity modulation of FcγRIIA ligand binding by glycosphingolipids by inside-out signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKoshu Okubo,Michael D Brenner,Xavier Cullere,Gurpanna Saggu,Myra L Patchen,Nandita Bose,Saki Mihori,Zhou Yuan,Clifford A Lowell,Cheng Zhu,Tanya N MayadasPubMed 34010642
- Nature communications 11:55972020A universal dual mechanism immunotherapy for the treatment of influenza virus infections.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Liu,Boning Zhang,Yingcai Wang,Hanan S Haymour,Fenghua Zhang,Le-Cun Xu,Madduri Srinivasarao,Philip S LowPubMed 33154358
- Cell reports 32:1079182020Human-IgG-Neutralizing Monoclonal Antibodies Block the SARS-CoV-2 Infection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJinkai Wan et. al.PubMed 32668215
- Cancer cell 37:850-866.e72020Isotype Switching Converts Anti-CD40 Antagonism to Agonism to Elicit Potent Antitumor Activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojie Yu,H T Claude Chan,Hayden Fisher,Christine A Penfold,Jinny Kim,Tatyana Inzhelevskaya,C Ian Mockridge,Ruth R French,Patrick J Duriez,Leon R Douglas,Vikki English,J Sjef Verbeek,Ann L White,Ivo Tews,Martin J Glennie,Mark S CraggPubMed 32442402
- Nature communications 11:12532020Identification of two major autoantigens negatively regulating endothelial activation in Takayasu arteritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTomoyuki Mutoh et. al.PubMed 32152303
- Gut microbes 11:405-4202019Dynamic immunoglobulin responses to gut bacteria during inflammatory bowel disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSunaina Rengarajan,Emily E Vivio,Miles Parkes,Daniel A Peterson,Elisha D O Roberson,Rodney D Newberry,Matthew A Ciorba,Chyi-Song HsiehPubMed 31203722
- Clinical cancer research : an official journal of the American Association for Cancer Research 25:3630-36422019A Novel mTORC1/2 Inhibitor (MTI-31) Inhibits Tumor Growth, Epithelial-Mesenchymal Transition, Metastases, and Improves Antitumor Immunity in Preclinical Models of Lung Cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianwen Zhang,Yan Zhang,Yaqing Chen,Jianchang Qian,Xuesai Zhang,Ker YuPubMed 30796032
Key facts
- Host species
- Goat
- Target species
- Human
- Target isotype
- IgG
- Target specificity
- Fc region
- Minimal cross-reactivity
- Mouse, Rat
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Applications
- Flow Cyt
- Clonality
- Polyclonal
- Format
- F(ab')2
Abcam Recommends
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application Flow Cyt | Reactivity Reacts | Dilution info 1/50.00000 - 1/200.00000 | Notes - |
Target data
Function
Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268). Mediates IgG effector functions on monocytes triggering ADCC of virus-infected cells.
Additional Targets
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Alternative names
Immunoglobulin heavy constant gamma 1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE, IGHG1
Recommended products
Goat F(ab')2 Anti-Human IgG - Fc (PE) is a pre-adsorbed secondary antibody with a maximum absorption wavelength of 480;565nm and a maximum emission wavelength of 578nm. Suitable for Flow Cytometry.
- Preadsorbed to prevent cross-reactivity and reduce background staining
- F(ab) fragment secondary antibodies eliminate non-specific binding between Fc regions of antibodies and Fc receptors on immune cells
- Cited in over 10 publications
Key facts
- Description
- Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed
- Host species
- Goat
- Target species
- Human
- Target isotype
- IgG
- Target specificity
- Fc region
- Minimal cross-reactivity
- Mouse, Rat
- Conjugation
- PE
- Excitation/Emission
- Ex: 480;565nm, Em: 578nm
- Applications
- Flow Cyt
- Clonality
- Polyclonal
- Format
- F(ab')2
- Pre-adsorbed
- Yes
- Isotype
- IgG
- Concentration
Properties
- Form
- Liquid
- Storage buffer
pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA- Purification technique
- Affinity purification Immunogen
- Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Phycoerythrin.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
By immunoelectrophoresis and ELISA this antibody reacts specifically with human IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to mouse and rat IgG was detected. This antibody may cross react with IgG from other species.
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1 product image
Flow Cytometry - Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)
Flow Cytometry - Goat F(ab')2 Anti-Human IgG - Fc (PE), pre-adsorbed (ab98596)CHO cell line expressing membrane bound human TNFα (stable transfectants) was incubated with 10 μg/ml Remicade (anti-human TNFa monoclonal antibody) for 1 h in 4°C. The unbound antibody was washed off by centrifugation (300x g for 5 min) and binding of remicade was detected with PE Goat F(ab)2 anti-hIgG Fc (ab98596) – 1:100 (5 μg/ml), 30 min incubation in 4°C. The cells were washed twice in FACS buffer (2.5% BSA, 0.1% sodium azide in dPBS), before flow cytometric analysis.
PE goat F(ab)2 anti-hIgG detected binding of remicade to TNFα CHO cell line giving strong positive signal, however there was some non-specific binding to the cells alone. Further optimisation of the reagent concentration and washing procedure should improve the background signal.
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