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Suitable for ICC, IHC-P, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining.

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Key facts

Host species

Goat

Target species

Human

Target isotype

IgG

Target specificity

Fc region

Minimal cross-reactivity

Mouse, Rat

Applications

ICC, IHC-P, ELISA, WB

Clonality

Polyclonal

Format

F(ab')2

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Reactivity data

Application

ICC

Reactivity

Reacts

Dilution info

-

Notes

-

Application

IHC-P

Reactivity

Reacts

Dilution info

1/200 - 1/2000

Notes

-

Application

ELISA

Reactivity

Reacts

Dilution info

1/100 - 1/30000

Notes

If used for coating, dilute 1/100 - 1/500.

Application

WB

Reactivity

Reacts

Dilution info

1/1000 - 1/30000

Notes

(chemiluminescece) or 1/1000 - 1/10000 (colorimetric).

Target data

Function

Constant region of immunoglobulin heavy chains. Immunoglobulins, also known as antibodies, are membrane-bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound immunoglobulins serve as receptors which, upon binding of a specific antigen, trigger the clonal expansion and differentiation of B lymphocytes into immunoglobulins-secreting plasma cells. Secreted immunoglobulins mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:22158414, PubMed:20176268). The antigen binding site is formed by the variable domain of one heavy chain, together with that of its associated light chain. Thus, each immunoglobulin has two antigen binding sites with remarkable affinity for a particular antigen. The variable domains are assembled by a process called V-(D)-J rearrangement and can then be subjected to somatic hypermutations which, after exposure to antigen and selection, allow affinity maturation for a particular antigen (PubMed:17576170, PubMed:20176268).

Alternative names

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Suitable for ICC, IHC-P, ELISA, WB. Preadsorbed to minimise non-specific binding and high background staining.

Alternative names

Key facts

Description

Goat F(ab')2 Anti-Human IgG - Fc, pre-adsorbed

Host species

Goat

Target species

Human

Target isotype

IgG

Target specificity

Fc region

Minimal cross-reactivity

Mouse, Rat

Applications

ICC, IHC-P, ELISA, WB

Clonality

Polyclonal

Format

F(ab')2

Pre-adsorbed

Yes

Isotype

IgG

Concentration
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Properties

Form

Liquid

Storage buffer

pH: 6.8 - 7.4
Preservative: 0.09% Sodium azide
Constituents: PBS

Purification technique

Affinity purification Immunogen

Purification notes

This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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    Product protocols

    For this product, it's our understanding that no specific protocols are required. You can:

    Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

    For licensing inquiries, please contact partnerships@abcam.com

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