AB5887

Goat F(ab')2 Anti-Mouse IgG - F(ab')2 (HRP), pre-adsorbed

Name: Goat F(ab')2 Anti-Mouse IgG - F(ab')2 (HRP), pre-adsorbed secondary antibody (ab5887) | Abcam
Brand: Abcam Limited
SKU: AB5887
Price: 295 USD
Availability: InStock

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(7 Publications)

Suitable for Dot, IM, IHC, ICC, ELISA, WB. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 7 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

F(ab')2

Minimal cross-reactivity

Rat, Sheep, Rabbit, Horse, Cow, Human,

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

ELISA, WB, IHC, IM, Dot, ICC

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2500", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/130000", "notes":"This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature." }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/20000", "notes":"" } } }

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Properties and storage information

Form

Lyophilized

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage buffer

pH: 7.2 Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (7)

Recent publications for all applications. Explore the full list and refine your search

iScience 26:107731 PubMed37701577

2023

Cryptotanshinone is a candidate therapeutic agent for interstitial lung disease associated with a BRICHOS-domain mutation of .

Applications

Unspecified application

Species

Unspecified reactive species

Motoyasu Hosokawa,Ryuta Mikawa,Atsuko Hagiwara,Yukiko Okuno,Tomonari Awaya,Yuki Yamamoto,Senye Takahashi,Haruka Yamaki,Mitsujiro Osawa,Yasuhiro Setoguchi,Megumu K Saito,Shinji Abe,Toyohiro Hirai,Shimpei Gotoh,Masatoshi Hagiwara

International journal of molecular sciences 23: PubMed36430510

2022

Deciphering the Role and Signaling Pathways of PKCα in Luminal A Breast Cancer Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Emilio M Serrano-López,Teresa Coronado-Parra,Consuelo Marín-Vicente,Zoltan Szallasi,Victoria Gómez-Abellán,María José López-Andreo,Marcos Gragera,Juan C Gómez-Fernández,Rubén López-Nicolás,Senena Corbalán-García

Nature communications 12:1792 PubMed33741926

2021

Red blood cell mannoses as phagocytic ligands mediating both sickle cell anaemia and malaria resistance.

Applications

Unspecified application

Species

Unspecified reactive species

Huan Cao,Aristotelis Antonopoulos,Sadie Henderson,Heather Wassall,John Brewin,Alanna Masson,Jenna Shepherd,Gabriela Konieczny,Bhinal Patel,Maria-Louise Williams,Adam Davie,Megan A Forrester,Lindsay Hall,Beverley Minter,Dimitris Tampakis,Michael Moss,Charlotte Lennon,Wendy Pickford,Lars Erwig,Beverley Robertson,Anne Dell,Gordon D Brown,Heather M Wilson,David C Rees,Stuart M Haslam,J Alexandra Rowe,Robert N Barker,Mark A Vickers

The Journal of clinical investigation 130:5817-5832 PubMed32750042

2020

Dominant-negative NFKBIA mutation promotes IL-1β production causing hepatic disease with severe immunodeficiency.

Applications

Unspecified application

Species

Unspecified reactive species

Enrica Ek Tan,Richard A Hopkins,Chrissie K Lim,Saumya S Jamuar,Christina Ong,Koh C Thoon,Mark Ja Koh,Eun Mong Shin,Derrick Wq Lian,Madhushanee Weerasooriya,Christopher Zw Lee,Andreas Alvin Pumomo Soetedjo,Chang Siang Lim,Veonice B Au,Edmond Chua,Hui Yin Lee,Leigh Ann Jones,Sharmy S James,Nivashini Kaliaperumal,Jeffery Kwok,Ee Shien Tan,Biju Thomas,Lynn Xue Wu,Lena Ho,Anna Marie Fairhurst,Florent Ginhoux,Adrian Kk Teo,Yong Liang Zhang,Kok Huar Ong,Weimiao Yu,Byrappa Venkatesh,Vinay Tergaonkar,Bruno Reversade,Keh Chuang Chin,Ah Moy Tan,Woei Kang Liew,John E Connolly

iScience 13:229-242 PubMed30870781

2019

Loss of RNA-Binding Protein Sfpq Causes Long-Gene Transcriptopathy in Skeletal Muscle and Severe Muscle Mass Reduction with Metabolic Myopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Motoyasu Hosokawa,Akihide Takeuchi,Jun Tanihata,Kei Iida,Shin'ichi Takeda,Masatoshi Hagiwara

Nature communications 7:11391 PubMed27102360

2016

Selective inhibition of the kinase DYRK1A by targeting its folding process.

Applications

Unspecified application

Species

Unspecified reactive species

Isao Kii,Yuto Sumida,Toshiyasu Goto,Rie Sonamoto,Yukiko Okuno,Suguru Yoshida,Tomoe Kato-Sumida,Yuka Koike,Minako Abe,Yosuke Nonaka,Teikichi Ikura,Nobutoshi Ito,Hiroshi Shibuya,Takamitsu Hosoya,Masatoshi Hagiwara

PLoS pathogens 8:e1002655 PubMed22536153

2012

CLEC5A regulates Japanese encephalitis virus-induced neuroinflammation and lethality.

Applications

Unspecified application

Species

Unspecified reactive species

Szu-Ting Chen,Ren-Shyan Liu,Ming-Fang Wu,Yi-Ling Lin,Se-Yi Chen,David Tat-Wei Tan,Teh-Ying Chou,I-Shuen Tsai,Lei Li,Shie-Liang Hsieh

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Goat F(ab')2 Anti-Mouse IgG - F(ab')2 (HRP), pre-adsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (7)

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