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Suitable for Dot, IM, IHC, ICC/IF, ELISA, WB. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 7 publications.

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Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

F(ab')2

Minimal cross-reactivity

Rat, Sheep, Rabbit, Horse, Cow, Human

Conjugation

HRP

Applications

Dot, IM, IHC, ICC/IF, ELISA, WB

Clonality

Polyclonal

Format

F(ab')2

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Reactivity data

Application
Dot
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IM
Reactivity
Reacts
Dilution info
-
Notes

-

Application
IHC
Reactivity
Reacts
Dilution info
-
Notes

-

Application
ICC/IF
Reactivity
Reacts
Dilution info
1/500.00000 - 1/2500.00000
Notes

-

Application
ELISA
Reactivity
Reacts
Dilution info
1/130000
Notes

This product has been assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature.

Application
WB
Reactivity
Reacts
Dilution info
1/2000.00000 - 1/20000.00000
Notes

-

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Suitable for Dot, IM, IHC, ICC/IF, ELISA, WB. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 7 publications.

Key facts

Description

Goat F(ab')2 Anti-Mouse IgG - F(ab')2 (HRP), pre-adsorbed

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

F(ab')2

Minimal cross-reactivity

Rat, Sheep, Rabbit, Horse, Cow, Human

Conjugation

HRP

Applications

Dot, IM, IHC, ICC/IF, ELISA, WB

Clonality

Polyclonal

Format

F(ab')2

Pre-adsorbed

Yes

Specificity

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse, Human, Rabbit, Rat and Sheep Serum Proteins.

Isotype

IgG

Concentration
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Properties

Form

Liquid

Storage buffer

pH: 6.5
Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.87% Sodium chloride, 0.42% Tripotassium orthophosphate

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

Downloads

Fluorochrome chart

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

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