Suitable for WB, Dot, Flow Cyt, IM, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Expected | Dilution info 1/100 - 1/500 | Notes - |
Application Dot | Reactivity Expected | Dilution info - | Notes - |
Application Flow Cyt | Reactivity Reacts | Dilution info 1/100.00000 - 1/250.00000 | Notes The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. |
Application IM | Reactivity Reacts | Dilution info - | Notes Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity. |
Application ICC/IF | Reactivity Reacts | Dilution info 1/100.00000 - 1/250.00000 | Notes - |
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Igh-4, Ighg1, Ig gamma-1 chain C region secreted form
Suitable for WB, Dot, Flow Cyt, IM, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.
No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse or Human Serum Proteins.
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution
F(ab')2 fragment PE conjugated Anti-Mouse IgG F(ab')2 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.
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