AB5889

Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (PE), pre-adsorbed

Name: Goat F(ab')2 Anti Mouse (IgG (Fab')2) secondary antibody preadsorbed PE (ab5889)
Brand: Abcam Limited
SKU: AB5889
Price: 295 USD
Availability: InStock

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(3 Publications)

Suitable for WB, Dot, Flow Cyt, IM, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 3 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

F(ab')2

Minimal cross-reactivity

Rat, Sheep, Rabbit, Horse, Cow, Human

Pre-adsorbed

Yes

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Applications

Dot, ICC/IF, IM, WB, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse or Human Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/100 - 1/500", "notes":"" }, "Dot": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/250", "notes":"The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining." }, "IM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity." }, "ICC/IF": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/100 - 1/250", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

F(ab')2 fragment PE conjugated Anti-Mouse IgG F(ab')2 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in immunology 15:1282680 PubMed38318189

2024

Immune evasion by proteolytic shedding of natural killer group 2, member D ligands in infection.

Applications

Unspecified application

Species

Unspecified reactive species

Margit Anthofer,Markus Windisch,Rosa Haller,Sandra Ehmann,Sebastian Wrighton,Michael Miller,Lorenz Schernthanner,Iris Kufferath,Silvia Schauer,Barbara Jelušić,Sabine Kienesberger,Ellen L Zechner,Gernot Posselt,Mar Vales-Gomez,Hugh T Reyburn,Gregor Gorkiewicz

Stem cell research & therapy 8:246 PubMed29096715

2017

Differentiation of human umbilical cord Wharton's jelly-derived mesenchymal stem cells into endometrial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qin Shi,JingWei Gao,Yao Jiang,Baolan Sun,Wei Lu,Min Su,Yunzhao Xu,Xiaoqing Yang,Yuquan Zhang

Journal of virology 85:10741-54 PubMed21849462

2011

Encephalomyocarditis virus 2A protein is required for viral pathogenesis and inhibition of apoptosis.

Applications

Unspecified application

Species

Unspecified reactive species

M Carocci,N Cordonnier,H Huet,A Romey,A Relmy,K Gorna,S Blaise-Boisseau,S Zientara,L Bakkali Kassimi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (PE), pre-adsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (3)

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