Goat F(ab')2 Anti Mouse (IgG (Fab')2) secondary antibody preadsorbed PE (ab5889)

Suitable for WB, Dot, Flow Cyt, IM, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

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Alternative names=Ig gamma-1 chain C region secreted form, Ighg1, Igh-4

Reactivity data

Application	Reactivity	Dilution info	Notes
Application WB	Reactivity Expected	Dilution info 1/100 - 1/500	Notes -
Application Dot	Reactivity Expected	Dilution info -	Notes -
Application Flow Cyt	Reactivity Reacts	Dilution info 1/100.00000 - 1/250.00000	Notes The maximum amount of reagent required to stain 1 x 10⁶ cells in flow cytometry is approximately 1.0 µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining.
Application IM	Reactivity Reacts	Dilution info -	Notes Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity.
Application ICC/IF	Reactivity Reacts	Dilution info 1/100.00000 - 1/250.00000	Notes -

Suitable for WB, Dot, Flow Cyt, IM, ICC/IF. Preadsorbed to minimise non-specific binding and high background staining. Cited in 2 publications.

Alternative names=Ig gamma-1 chain C region secreted form, Ighg1, Igh-4

Description: Goat F(ab')2 Anti-Mouse IgG - (Fab)'2 (PE), pre-adsorbed
Host species: Goat
Target species: Mouse
Target isotype: IgG
Target specificity: F(ab')2
Minimal cross-reactivity: Rat, Sheep, Rabbit, Horse, Cow, Human
Conjugation: PE
Excitation/Emission: Ex: 480;565nm, Em: 578nm
Applications: WB, Dot, Flow Cyt, IM, ICC/IF
Clonality: Polyclonal
Format: F(ab')2
Pre-adsorbed: Yes
Specificity: No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(c) or Bovine, Horse or Human Serum Proteins.
Isotype: IgG
Concentration

Form: Liquid
Storage buffer: pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 1% BSA, 0.878% Sodium chloride, 0.424% Tripotassium orthophosphate
Purification notes: F(ab')2 fragment PE conjugated Anti-Mouse IgG F(ab')2 was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

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