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AB98741

Goat F(ab')2 Anti-Mouse IgG - Fc (HRP), pre-adsorbed

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Suitable for ICC, IHC-P, WB, ELISA. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Fc region

Minimal cross-reactivity

Rat, Human

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission
Applications

ELISA, ICC, IHC-P, WB

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with mouse IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human and rat IgG was detected. This antibody may cross react with IgG from other species.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/2000", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/2000 - 1/25000", "notes":"<p>Colorimetric: Use 1/2,000-1/20,000 dilution; Chemiluminescent: Use 1/5,000-1/25,000 dilution.</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/100000", "notes":"<p>Primary dilution.</p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Horse Radish Peroxidase.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.05% CMIT/MIT based preservative Constituents: 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Product protocols

Target data

See full target information Ighg1

Additional targets

Ighg1

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