AB5881

Goat F(ab')2 Anti-Mouse IgG - Fc (PE), pre-adsorbed

Name: Goat F(ab')2 Anti Mouse (IgG Fc) secondary antibody preadsorbed PE (ab5881)
Brand: Abcam Limited
SKU: AB5881

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(4 Publications)

Suitable for IM, ICC/IF, Flow Cyt. Preadsorbed to minimise non-specific binding and high background staining. Cited in 4 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form, Igh-4, Ighg1

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Fc region

Minimal cross-reactivity

Horse, Cow, Human

Pre-adsorbed

Yes

Conjugation

Excitation/Emission

Ex: 480;565nm, Em: 578nm

Applications

ICC/IF, Flow Cyt, IM

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

No reaction was observed against anti-Pepsin, anti-Goat IgG F(c), Mouse IgG F(ab) or Bovine, Horse or Human Serum Proteins.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IM": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"", "notes":"" }, "ICC/IF": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/100 - 1/250", "notes":"" }, "Flow Cyt": { "reactivity":"NO_EXPERIMENTAL_DATA_EXPECTED_TO_REACT", "dilution-info":"1/100 - 1/250", "notes":"The maximum amount of reagent required to stain 1 x 106 cells in Flow Cytometry is approximately 1.0µg of antibody conjugate. Lesser amounts of reagent may be sufficient for staining. As a general guideline dilutions of 1/50 to 1/200 should be suitable for most applications.Suitable for other antibody based fluorescent assays requiring extremely low background levels, absence of F(c) mediated binding, lot-to-lot consistency, high titer and specificity." } } }

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Properties and storage information

Form

Liquid

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Autophagy : PubMed33034545

2020

Raft-like lipid microdomains drive autophagy initiation via AMBRA1-ERLIN1 molecular association within MAMs.

Applications

Unspecified application

Species

Unspecified reactive species

Valeria Manganelli,Paola Matarrese,Manuela Antonioli,Lucrezia Gambardella,Tiziana Vescovo,Christine Gretzmeier,Agostina Longo,Antonella Capozzi,Serena Recalchi,Gloria Riitano,Roberta Misasi,Joern Dengjel,Walter Malorni,Gian Maria Fimia,Maurizio Sorice,Tina Garofalo

Oncology reports 40:3297-3312 PubMed30542711

2018

Cellular communication promotes mammosphere growth and collective invasion through microtubule‑like structures and angiogenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Shangke Huang,Na Yuan,Guanying Wang,Fang Wu,Lu Feng,Minna Luo,Miao Li,Anqi Luo,Xinhan Zhao,Lingxiao Zhang

Experimental and therapeutic medicine 14:4125-4133 PubMed29104629

2017

Association between B7-H1 and cervical cancer: B7-H1 impairs the immune response in human cervical cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jianying Tao,Jianrong Dai,Shunyu Hou

Journal of immunology (Baltimore, Md. : 1950) 194:5320-8 PubMed25911748

2015

Viremic HIV Controllers Exhibit High Plasmacytoid Dendritic Cell-Reactive Opsonophagocytic IgG Antibody Responses against HIV-1 p24 Associated with Greater Antibody Isotype Diversification.

Applications

Unspecified application

Species

Unspecified reactive species

M Christian Tjiam,James P A Taylor,Mazmah A Morshidi,Lucy Sariputra,Sally Burrows,Jeffrey N Martin,Steven G Deeks,Dino B A Tan,Silvia Lee,Sonia Fernandez,Martyn A French

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat F(ab')2 Anti-Mouse IgG - Fc (PE), pre-adsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Specificity

Alternative Products

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Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (4)

Product promise