AB5870

Goat F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed

Name: Goat F(ab')2 Anti Mouse (IgG) secondary antibody preadsorbed HRP (ab5870)
Brand: Abcam Limited
SKU: AB5870

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(12 Publications)

Suitable for Dot, EM, IHC, WB, ELISA, ICC. Ideal for western blot. Preadsorbed to minimise non-specific binding and high background staining. Cited in 12 publications.

View Alternative Names

Igh-4, Ighg1, Ig gamma-1 chain C region secreted form

Key facts

Host species

Goat

Target species

Mouse

Target isotype

IgG

Target specificity

Heavy & Light chains

Minimal cross-reactivity

Rat, Sheep, Rabbit, Horse, Cow, Human

Pre-adsorbed

Yes

Conjugation

HRP

Excitation/Emission

Applications

EM, Dot, WB, IHC, ICC, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "Dot": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "EM": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "IHC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/1000 - 1/10000", "notes":"" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/60000", "notes":"" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/2500", "notes":"" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Purification notes

This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation.

Storage buffer

Preservative: 0.01% Gentamicin sulphate Constituents: 1% BSA, 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Product protocols

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information Ighg1

Additional targets

Ighg1

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 32:995-1005 PubMed39934431

2025

Promoter-proximal RNA polymerase II termination regulates transcription during human cell type transition.

Applications

Unspecified application

Species

Unspecified reactive species

Kseniia Lysakovskaia,Arjun Devadas,Björn Schwalb,Michael Lidschreiber,Patrick Cramer

Vaccines 11: PubMed38006010

2023

Pre-Clinical Development of an Adenovirus Vector Based RSV and Shingles Vaccine Candidate.

Applications

Unspecified application

Species

Unspecified reactive species

Lawrence Petherbridge,Charlotte Davis,Angela Robinson,Thomas Evans,Sarah Sebastian

The Journal of experimental biology 226: PubMed37497728

2023

Intergenerational plasticity to cycling high temperature and hypoxia affects offspring stress responsiveness and tolerance in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Y-T Lim,Nicholas J Bernier

The Journal of experimental biology 225: PubMed36326068

2022

Zebrafish parental progeny investment in response to cycling thermal stress and hypoxia: deposition of heat shock proteins but not cortisol.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Y-T Lim,Nicholas J Bernier

G3 (Bethesda, Md.) 12: PubMed35253846

2022

RNA transcription and degradation of Alu retrotransposons depends on sequence features and evolutionary history.

Applications

Unspecified application

Species

Unspecified reactive species

Till Baar,Sebastian Dümcke,Saskia Gressel,Björn Schwalb,Alexander Dilthey,Patrick Cramer,Achim Tresch

Nature 598:368-372 PubMed34526721

2021

Structural basis of human transcription-DNA repair coupling.

Applications

Unspecified application

Species

Unspecified reactive species

Goran Kokic,Felix R Wagner,Aleksandar Chernev,Henning Urlaub,Patrick Cramer

The EMBO journal 40:e107015 PubMed33555055

2021

Transcription activation depends on the length of the RNA polymerase II C-terminal domain.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Sawicka,Gabriel Villamil,Michael Lidschreiber,Xavier Darzacq,Claire Dugast-Darzacq,Björn Schwalb,Patrick Cramer

Science (New York, N.Y.) 371:305-309 PubMed33446560

2021

Structure of a transcribing RNA polymerase II-U1 snRNP complex.

Applications

Unspecified application

Species

Unspecified reactive species

Suyang Zhang,Shintaro Aibara,Seychelle M Vos,Dmitry E Agafonov,Reinhard Lührmann,Patrick Cramer

Nature 560:607-612 PubMed30135578

2018

Structure of activated transcription complex Pol II-DSIF-PAF-SPT6.

Applications

Unspecified application

Species

Unspecified reactive species

Seychelle M Vos,Lucas Farnung,Marc Boehning,Christoph Wigge,Andreas Linden,Henning Urlaub,Patrick Cramer

Conservation physiology 4:cow036 PubMed27757235

2016

Pass the salt: physiological consequences of ecologically relevant hyposmotic exposure in juvenile gummy sharks () and school sharks ().

Applications

Unspecified application

Species

Unspecified reactive species

Andrea J Morash,Sara R C Mackellar,Louise Tunnah,David A Barnett,Kilian M Stehfest,Jayson M Semmens,Suzanne Currie

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Goat F(ab')2 Anti-Mouse IgG - H&L (HRP), pre-adsorbed

Key facts

Host species

Target species

Target isotype

Target specificity

Minimal cross-reactivity

Pre-adsorbed

Conjugation

Excitation/Emission

Applications

Clonality

Isotype

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Product protocols

Target data

Additional targets

Publications (12)

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