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AB102290

Goat F(ab')2 Anti-Rabbit IgG - (Fab')2 (Biotin), pre-adsorbed

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Suitable for WB, ELISA, IHC-P, ICC. Preadsorbed to minimise non-specific binding and high background staining.

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

F(ab')2

Minimal cross-reactivity

Mouse, Rat, Human

Pre-adsorbed

Yes

Conjugation

Biotin

Excitation/Emission
Applications

IHC-P, ICC, WB, ELISA

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"<p>(Chemiluminescent).</p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/10000 - 1/200000", "notes":"<p>(Primary).</p>" }, "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/500 - 1/5000", "notes":"<p></p>" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/200 - 1/1000", "notes":"<p></p>" } } }

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies.The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to Biotin.
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Product protocols

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