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AB102294

Goat F(ab')2 Anti-Rabbit IgG - (Fab')2 (DyLight® 650), pre-adsorbed

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(1 Publication)

Suitable for IHC-P, Flow Cyt, ICC. Preadsorbed to minimise non-specific binding and high background staining. Cited in 1 publication.

View Alternative Names

Ig gamma 1 chain C region, Ig gamma 3 chain C region, Ig gamma 4 chain C region, Ig gamma chain C region, Ig gamma-2 chain C region, Ig kappa-b4 chain C region, Ig kappa-b5 chain C region, Ig kappa-b9 chain C region, Ig lambda chain C region, Immunoglobin heavy constant gamma 1, Immunoglobulin G, K-BAS

Key facts

Host species

Goat

Target species

Rabbit

Target isotype

IgG

Target specificity

F(ab')2

Minimal cross-reactivity

Mouse, Rat, Human

Pre-adsorbed

Yes

Conjugation

DyLight® 650

Excitation/Emission

Ex: 654nm, Em: 673nm

Applications

ICC, IHC-P, Flow Cyt

applications

Clonality

Polyclonal

Isotype

IgG

Specificity

<p>By immunoelectrophoresis and ELISA this antibody reacts specifically with rabbit IgG. Cross reactivity with IgA and IgM is negligible. No antibody was detected against non-immunoglobulin serum proteins. Less than 1% cross reactivity to human, mouse and rat IgG was detected. This antibody may cross react with IgG from other species.</p>

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Alternative Products

Secondary Antibodies

AB6717

Goat Anti-Rabbit IgG H&L (FITC)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (FITC) (AB6717)

  • 5
  • 2
Secondary Antibodies

AB175471

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) (AB175471)

  • 5
  • 1
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Secondary Antibodies

AB150079

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (AB150079)

  • 3
  • 1
Secondary Antibodies

AB150080

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) (AB150080)

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  • 3
Secondary Antibodies

AB205718

Goat Anti-Rabbit IgG H&L (HRP)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Rabbit IgG H&L (HRP) (AB205718)

  • 5
  • 8

Complementary Products

Secondary Antibodies

AB6108

Goat F(ab')2 Anti-Rabbit IgG - (Fab)'2 (FITC), pre-adsorbed

  • 0
  • 0
Secondary Antibodies

AB98757

Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (DyLight® 488), pre-adsorbed

  • 0
  • 0
Secondary Antibodies

AB98759

Goat F(ab')2 Anti-Mouse IgG - (Fab')2 (DyLight® 594), pre-adsorbed

  • 0
  • 0
Reagents, Controls & Accessories

AB7481

Normal Goat Serum

Immunohistochemistry (Frozen sections) - Normal Goat Serum (AB7481)

  • 5
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "IHC-P": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" }, "Flow Cyt": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/200", "notes":"<p></p>" }, "ICC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"1/50 - 1/500", "notes":"<p></p>" } } }
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Product details

DyLight® 650 replaces DyLight® 649.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Antiserum was solid phase adsorbed to ensure specificity. Antiserum was cross adsorbed using human, mouse and rat immunosorbents to remove cross reactive antibodies. The antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragments were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 650.
Storage buffer
pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: PBS, 0.2% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

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Target data

See full target information Rabbit IgG
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 26:1121-1133 PubMed32881314

2020

Dopamine neurons in the ventral periaqueductal gray modulate isoflurane anesthesia in rats.

Applications

Unspecified application

Species

Unspecified reactive species

Chengxi Liu,Xiao Zhou,Qiuyu Zhu,Bao Fu,Song Cao,Yu Zhang,Lin Zhang,Yi Zhang,Tian Yu
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Product promise

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